Epidermal growth factor (EGF)-induced morphological changes in the basement membrane of chick embryonic skin

1988 ◽  
Vol 254 (3) ◽  
Author(s):  
Yoshihiro Akimoto ◽  
Akiko Obinata ◽  
Hiroyoshi Endo ◽  
Hiroshi Hirano
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Basement Membrane ◽  
Morphological Changes ◽  
Embryonic Skin ◽  
Epidermal Growth ◽  
Chick Embryonic Skin

scholarly journals Coactivation of Rac1 and Cdc42 at Lamellipodia and Membrane Ruffles Induced by Epidermal Growth Factor

2004 ◽  
Vol 15 (3) ◽  
pp. 1003-1010 ◽  
Author(s):  
Kazuo Kurokawa ◽  
Reina E. Itoh ◽  
Hisayoshi Yoshizaki ◽  
Yusuke Ohba Takeshi Nakamura ◽  
Michiyuki Matsuda
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Morphological Changes ◽  
Critical Role ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
A431 Cells ◽  
Major Function ◽  
Membrane Ruffling ◽  
Epidermal Growth

A major function of Rho-family GTPases is to regulate the organization of the actin cytoskeleton; filopodia, lamellipodia, and stress fiber are regarded as typical phenotypes of the activated Cdc42, Rac, and Rho, respectively. Using probes based on fluorescent resonance energy transfer, we report on the spatiotemporal regulation of Rac1 and Cdc42 at lamellipodia and membrane ruffles. In epidermal growth factor (EGF)-stimulated Cos1 and A431 cells, both Rac1 and Cdc42 were activated diffusely at the plasma membrane, followed by lamellipodial protrusion and membrane ruffling. Although Rac1 activity subsided rapidly, Cdc42 activity was sustained at lamellipodia. A critical role of Cdc42 in these EGF-induced morphological changes was demonstrated as follows. First, phorbol 12-myristate 13-acetate, which activated Rac1 but not Cdc42, could not induce full-grown lamellipodia in Cos1 cells. Second, a GTPase-activating protein for Cdc42, KIAA1204/CdGAP, inhibited lamellipodial protrusion and membrane ruffling without interfering with Rac1 activation. Third, expression of the Cdc42-binding domain of N-WASP inhibited the EGF-induced morphological changes. Therefore, Rac1 and Cdc42 seem to synergistically induce lamellipodia and membrane ruffles in EGF-stimulated Cos1 cells and A431 cells.

scholarly journals Morphological Changes in the Skin and Wool Fibres of Merino Sheep Infused with Mouse Epidermal Growth Factor

1983 ◽  
Vol 36 (4) ◽  
pp. 419 ◽  
Author(s):  
DE Hollis ◽  
RE Chapman ◽  
BA Panaretto ◽  
GPM Moore
Keyword(s):  
Electron Microscope ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Scanning Electron Microscope ◽  
Intravenous Infusion ◽  
Morphological Changes ◽  
Merino Sheep ◽  
Epidermal Growth ◽  
Skin Samples ◽  
Scanning Electron

Intravenous infusion of 4�5-4�7 mg of mouse epidermal growth factor (mEGF) into nine castrated male Merino sheep for 26 h resulted in complete casting of the fleeces 6-8 days later. The morphological changes which occurred in the skin were studied in skin samples taken before infusion and at intervals between 1 hand 42 days after the infusion had begun. Wool fibres from the shed fleeces were examined with the scanning electron microscope.

scholarly journals Transgenic expression of epidermal growth factor and keratinocyte growth factor in beta-cells results in substantial morphological changes

1999 ◽  
Vol 162 (2) ◽  
pp. 167-175 ◽  
Author(s):  
ML Krakowski ◽  
MR Kritzik ◽  
EM Jones ◽  
T Krahl ◽  
J Lee ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Transgenic Mice ◽  
Beta Cells ◽  
Morphological Changes ◽  
Keratinocyte Growth Factor ◽  
Substantial Impact ◽  
Insulin Promoter ◽  
Epidermal Growth

The upregulation of a limited number of growth factors in our interferon-gamma transgenic model for regeneration within the pancreas lead us to propose that these factors are important during pancreatic regeneration. In this study, we have assessed the influence of two growth factors within the pancreas, epidermal growth factor (EGF) and keratinocyte growth factor (KGF), by ectopically expressing these proteins under the control of the human insulin promoter in transgenic mice. This beta-cell-targeted expression of either EGF or KGF resulted in significant morphological changes, including cellular proliferation and disorganized islet growth. Intercrossing the individual Ins-EGF and Ins-KGF transgenic mice resulted in more profound changes in pancreatic morphology including proliferation of pancreatic cells and extensive intra-islet fibrosis. Insulin-producing beta-cells were found in some of the ducts of older Ins-EGF and Ins-EGFxKGF transgenic mice, and amylase-producing cells were observed within the islet structures of the double transgenic mice. These data suggest that both EGF and KGF are capable of affecting pancreatic differentiation and growth, and that co-expression of these molecules in islets has a more substantial impact on the pancreas than does expression of either growth factor alone.

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