Expression of mitochondrial genes in fertile and sterile sugar beet cytoplasms with different nuclear fertility restorer genes

1991 ◽  
Vol 83 (2) ◽  
pp. 217-224 ◽  
Author(s):  
N. A. Dudareva ◽  
A. V. Popovsky ◽  
U. V. Kasjanova ◽  
S. G. Veprev ◽  
A. V. Mglinets ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Mitochondrial Genes ◽  
Fertility Restorer ◽  
Restorer Genes ◽  
Fertility Restorer Genes

scholarly journals Homologous recombination changes the context of Cytochrome b transcription in the mitochondrial genome of Silene vulgaris KRA

2018 ◽  
Author(s):  
Helena Štorchová ◽  
James D. Stone ◽  
Daniel B. Sloan ◽  
Oushadee Abeyawardana ◽  
Karel Müller ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Mitochondrial Genome ◽  
Rna Editing ◽  
Cytochrome B ◽  
Mitochondrial Genes ◽  
Mitochondrial Genomes ◽  
Silene Vulgaris ◽  
Fertility Restorer ◽  
Restorer Genes ◽  
Fertility Restorer Genes

AbstractBackgroundSilene vulgaris (bladder campion) is a gynodioecious species existing as two genders – male-sterile females and hermaphrodites. Cytoplasmic male sterility (CMS) is generally encoded by mitochondrial genes, which interact with nuclear fertility restorer genes. Mitochondrial genomes of this species vary in DNA sequence, gene order and gene content. Multiple CMS genes are expected to exist in S. vulgaris, but little is known about their molecular identity.ResultsWe assembled the complete mitochondrial genome from the haplotype KRA of S. vulgaris. It consists of five chromosomes, two of which recombine with each other. Two small non-recombining chromosomes exist in linear, supercoiled and relaxed circle forms. We compared the mitochondrial transcriptomes from females and hermaphrodites and confirmed the differentially expressed chimeric gene bobt as the strongest CMS candidate gene in S. vulgaris KRA. The chimeric gene bobt is co-transcribed with the Cytochrome b (cob) gene in some genomic configurations. The co-transcription of a CMS factor with an essential gene may constrain transcription inhibition as a mechanism for fertility restoration because of the need to maintain appropriate production of the necessary protein. Homologous recombination places the gene cob outside the control of bobt, which allows for the suppression the CMS gene by the fertility restorer genes. In addition, by analyzing RNA editing, we found the loss of three editing sites in the KRA mitochondrial genome and identified four sites with highly distinct editing rates between KRA and another S. vulgaris haplotypes (KOV). Three of these highly differentially edited sites were located in the transport membrane protein B (mttB) gene. They resulted in differences in MttB protein sequences between haplotypes despite completely identical gene sequences.ConclusionsFrequent homologous recombination events that are widespread in plant mitochondrial genomes may change chromosomal configurations and also the control of gene transcription including CMS gene expression. Posttranscriptional processes, e.g RNA editing shall be evaluated in evolutionary and co-evolutionary studies of mitochondrial genes, because they may change protein composition despite the sequence identity of the respective genes. The investigation of natural populations of wild species such as S. vulgaris are necessary to reveal important aspects of CMS missed in domesticated crops, the traditional focus of the CMS studies.

scholarly journals Mutator-Induced Mutations of the rf1 Nuclear Fertility Restorer of T-Cytoplasm Maize Alter the Accumulation of T-urfl3 Mitochondrial Transcripts

Genetics ◽  
1996 ◽  
Vol 143 (3) ◽  
pp. 1383-1394
Author(s):  
Roger P Wise ◽  
Carren L Dill ◽  
Patrick S Schnable
Keyword(s):  
Pollen Fertility ◽  
Fertility Restoration ◽  
Mitochondrial Rna ◽  
Induced Mutations ◽  
Male Sterile ◽  
Fertility Restorer ◽  
Restorer Genes ◽  
Mitochondrial Transcripts ◽  
Ecori Restriction ◽  
Fertility Restorer Genes

Abstract Dominant alleles of the rf1 and rf2 nuclear-encoded fertility restorer genes are necessary for restoration of pollen fertility in T-cytoplasm maize. To further characterize fertility restoration mediated by the Rf1 allele, 123,500 gametes derived from plants carrying the Mutator transposable element family were screened for rf1-mutant alleles (rf1-m) Four heritable rf1-m alleles were recovered from these populations. Three rf1-m alleles were derived from the progenitor allele Rf1-IAl53 and one was derived from Rf1-Ky21. Cosegregation analysis revealed 5.5- and 2.4kb Mu1-hybridizing EcoRI restriction fragments in all of the male-sterile and none of the male-fertile plants in families segregating for rf1-m3207 and rf1-m3310, respectively. Mitochondrial RNA gel blot analyses indicated that all four rf1-m alleles in male-sterile plants cosegregated with the altered steady-state accumulation of 1.6 and O.6-kb T-urf13 transcripts, demonstrating that these transcripts are Rf1 dependent. Plants carrying a leaky mutant, rf1-m7323, revealed variable levels of Rf1-associated, T-urf13 transcripts and the degree of pollen fertility. The ability to obtain rf1-m derivatives from Rf1 indicates that Rf1 alleles produce a functional gene product necessary for the accumulation of specific T-urf13 transcripts in T-cytoplasm maize.

Development and utilization of an InDel marker linked to the fertility restorer genes of CMS-D8 and CMS-D2 in cotton

2020 ◽  
Vol 47 (2) ◽  
pp. 1275-1282
Author(s):  
Juanjuan Feng ◽  
Haiyong Zhu ◽  
Meng Zhang ◽  
Xuexian Zhang ◽  
Liping Guo ◽  
...  
Keyword(s):  
Indel Marker ◽  
Fertility Restorer ◽  
Restorer Genes ◽  
Development And Utilization ◽  
Fertility Restorer Genes

Correlated effects of exotic pollen-fertility restorer genes on agronomic and quality traits of hybrid rye

2017 ◽  
Vol 136 (2) ◽  
pp. 224-229 ◽  
Author(s):  
Thomas Miedaner ◽  
Cathérine Pauline Herter ◽  
Heike Goßlau ◽  
Peer Wilde ◽  
Bernd Hackauf
Keyword(s):  
Pollen Fertility ◽  
Quality Traits ◽  
Fertility Restorer ◽  
Restorer Genes ◽  
Hybrid Rye ◽  
Pollen Fertility Restorer ◽  
Fertility Restorer Genes

Molecular markers in identification of sunflower pollen fertility restorer genes

2009 ◽  
Vol 35 (6) ◽  
pp. 367-370 ◽  
Author(s):  
I. N. Anisimova ◽  
V. A. Gavrilova ◽  
V. T. Rozhkova ◽  
G. I. Timofeeva ◽  
M. A. Tikhonova
Keyword(s):  
Molecular Markers ◽  
Pollen Fertility ◽  
Fertility Restorer ◽  
Restorer Genes ◽  
Pollen Fertility Restorer ◽  
Fertility Restorer Genes

Male sterility in Indian mustard (Brassica juncea (L.) Coss). IV. Genetics of MS-4

1985 ◽  
Vol 27 (5) ◽  
pp. 487-490 ◽  
Author(s):  
Surinder S. Banga ◽  
K. S. Labana
Keyword(s):  
Male Sterility ◽  
Brassica Juncea ◽  
Indian Mustard ◽  
Field Trials ◽  
Genetic Constitution ◽  
Male Sterile ◽  
Fertility Restorer ◽  
Male Sterile Plant ◽  
Restorer Genes ◽  
Fertility Restorer Genes

Male sterile plants of Indian mustard (Brassica juncea (L.) Coss.) were observed in the F2 generation of the cross RLM-198 × EJ-33. The genetic analysis revealed that male sterility occurred when the cytoplasm of RLM-198 interacted with recessive nuclear genes of EJ-33. The genetic constitution of RLM-198 was postulated to be (S) RF RF, EJ-33 as (F) rf rf, and the male sterile plants as (S) rf rf. Varieties of Indian mustard from India mostly contained dominant fertility restorer genes, while European varieties had a greater frequency of the recessive maintainer genes. None of these varieties, however, was capable of complete maintenance of male sterility. Heterosis for yield up to 56% over the national check was observed in field trials. The use of this cytoplasmic male sterile plant in hybrid mustard production will not be economical, until a complete maintainer for male sterility is identified.Key words: Brassica juncea, Indian mustard, male sterility, hybrids.

Development and use of a two-gene marker-aided selection system for fertility restorer genes in rice

Euphytica ◽  
2006 ◽  
Vol 153 (1-2) ◽  
pp. 35-42 ◽  
Author(s):  
Majid Sattari ◽  
Arumugam Kathiresan ◽  
Glenn B. Gregorio ◽  
Jose E. Hernandez ◽  
Tamerlane M. Nas ◽  
...  
Keyword(s):  
Gene Marker ◽  
Selection System ◽  
Fertility Restorer ◽  
Restorer Genes ◽  
Fertility Restorer Genes

scholarly journals Identification of Suitable Restorers for WA-CMS Lines of Rice (Oryza sativa L.) through Conventional and Molecular Breeding Methods

2020 ◽  
Author(s):  
Anirban Nath ◽  
Disharee Nath ◽  
Chand Kumar Santra ◽  
Tapash Dasgupta
Keyword(s):  
Genetic Background ◽  
Molecular Breeding ◽  
Oryza Sativa L ◽  
Fertility Restoration ◽  
Male Sterile ◽  
Maintainer Line ◽  
Fertility Restorer ◽  
Restorer Genes ◽  
Restoration Potential ◽  
Fertility Restorer Genes

The current study extensively evaluates 51 genotypes for their fertility restoration potential using test crosses with five WA (wild abortive) Cytoplasmic Male Sterile lines namely IR58025A, IR6897A, IR79156A, IR80559A and APMS6A. Also the genotypes were screened using SSR markers RM6100 and RM10313, tightly linked with the fertility restorer genes Rf4 and Rf3 respectively. The two way approach helped in identifying potential restorers for five WA-CMS lines and also detected the presence of dominant Rf genes in their genetic background. The R-lines identified can be safely presumed to be strong restorers for consecutive A-lines they were crossed with. The study also identified a potential maintainer CN1039-9 for the A-line IR58025A. The maintainer line identified can be later exploited for developing new CMS lines.

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