Interrelationship between oxygen consumption, superoxide anion and hydrogen peroxide formation in phagocytosing guinea pig polymorphonuclear leucocytes

1979 ◽  
Vol 23 (2) ◽  
Author(s):  
DRI Pietro ◽  
Bellavite Paolo ◽  
Berton Giorgio ◽  
Rossi Filippo
Keyword(s):  
Hydrogen Peroxide ◽  
Oxygen Consumption ◽  
Guinea Pig ◽  
Superoxide Anion ◽  
Polymorphonuclear Leucocytes ◽  
Peroxide Formation ◽  
Hydrogen Peroxide Formation

β-lapachone enhancement of lipid peroxidation and superoxide anion and hydrogen peroxide formation by Sarcoma 180 ascites tumor cells

1979 ◽  
Vol 28 (6) ◽  
pp. 723-728 ◽  
Author(s):  
Roberto Docampo ◽  
Fernando S. Cruz ◽  
Alberto Boveris ◽  
Ramiro P.A. Muniz ◽  
Darci M.S. Esquivel
Keyword(s):  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Tumor Cells ◽  
Superoxide Anion ◽  
Sarcoma 180 ◽  
Ascites Tumor ◽  
Peroxide Formation ◽  
Hydrogen Peroxide Formation

Peroxidase associated with the root surface of Phaseolus vulgaris

1986 ◽  
Vol 64 (3) ◽  
pp. 573-578 ◽  
Author(s):  
F. G. Albert ◽  
L. W. Bennett ◽  
A. J. Anderson
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Anion ◽  
Indoleacetic Acid ◽  
Root Surface ◽  
Acid Oxidase ◽  
Peroxide Formation ◽  
Optimal Activity ◽  
Indoleacetic Acid Oxidase ◽  
Hydrogen Peroxide Formation ◽  
Intact Roots

The surface of bean roots demonstrates an intense peroxidase activity which was detected by hydrogen peroxide dependent formation of chromogen from chloronaphthol or dianisidine. Other peroxidase functions, oxidation of indoleacetic acid and NADPH, were catalysed by intact roots and were stimulated by Mn2+ and p-coumarate. Oxidation of NADPH involved superoxide anion [Formula: see text] and hydrogen peroxide formation. Molecular sizing chromatography of root washes demonstrated NADPH oxidase and peroxidase to be associated with higher weight components than indoleacetic acid oxidase. Root surface and root wash peroxidase displayed optimal activity between pH 7 and 8, whereas both sources of indoleacetic acid oxidase were more active at acidic pH. Native poly aery lamide gel electrophoresis of sterile root washes displayed two fast-moving anodic bands, whereas homogenates of the plant roots had several slower moving bands in addition.

Inhibition of the superoxide anion release and hydrogen peroxide formation in PMNLs by flavonolignans

2001 ◽  
Vol 15 (7) ◽  
pp. 608-612 ◽  
Author(s):  
Zsuzsa Varga ◽  
Andrea Czompa ◽  
Gy�rgy Kakuk ◽  
S�ndor Antus
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Anion ◽  
Peroxide Formation ◽  
Hydrogen Peroxide Formation

A New and Rapid Method for the Determination of Glucose by Measurement of Rate of Oxygen Consumption

1968 ◽  
Vol 14 (2) ◽  
pp. 116-131 ◽  
Author(s):  
Arnold Henry Kadish ◽  
Robert L Litle ◽  
James C Sternberg
Keyword(s):  
Hydrogen Peroxide ◽  
Oxygen Consumption ◽  
Glucose Oxidase ◽  
Oxygen Sensor ◽  
Direct Measure ◽  
Peroxide Formation ◽  
Glucose Levels ◽  
Rate Of Oxygen Consumption ◽  
Hydrogen Peroxide Formation

Abstract Glucose levels in serum, plasma, and urine are determined rapidly and conveniently by a new glucose oxidase method employing a polarographic oxygen sensor with a circuit modified to record the rate of oxygen consumption. The maximum apparent rate of oxygen consumption relative to the rate obtained with a glucose standard provides a direct measure of the glucose level in the sample; results are obtainable within 20 sec. after sample (100 µl.) addition and within 3 min. after a blood sample is withdrawn from a patient. Interferences associated with prior colorimetric glucose oxidase methods are avoided by measuring oxygen consumption instead of hydrogen peroxide formation. The method is described and results are presented showing a standard deviation of less than 1.5% on replicate determinations and a bias of 1% with respect to data obtained on the same samples by the automated ferricyanide method.

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