Effect of Escherichia coli lipopolysaccharide on the glucagon and insulin binding to isolated rat hepatocytes

1984 ◽  
Vol 65 (1) ◽  
Author(s):  
R. Pagani ◽  
M.T. Portol�s ◽  
A.M. Municio
Keyword(s):  
Escherichia Coli ◽  
Rat Hepatocytes ◽  
Insulin Binding ◽  
Isolated Rat Hepatocytes ◽  
Glucagon And Insulin ◽  
Escherichia Coli Lipopolysaccharide ◽  
Isolated Rat

The effects of acute and chronic dexamethasone administration on insulin binding to isolated rat hepatocytes and adipocytes

Metabolism ◽  
1975 ◽  
Vol 24 (4) ◽  
pp. 517-527 ◽  
Author(s):  
Jerrold M. Olefsky ◽  
Jennifer Johnson ◽  
Francis Liu ◽  
Phyllis Jen ◽  
Gerald M. Reaven
Keyword(s):  
Rat Hepatocytes ◽  
Insulin Binding ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

Mechanisms of the fasting-induced dissociation of insulin binding from its action in isolated rat hepatocytes

1984 ◽  
Vol 62 (1) ◽  
Author(s):  
Michael Trowbridge ◽  
Ann Sussman ◽  
Linda Ferguson ◽  
Boris Draznin ◽  
Naomi Neufeld ◽  
...  
Keyword(s):  
Rat Hepatocytes ◽  
Insulin Binding ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

Effects of epidermal growth factor (urogastrone) on gluconeogenesis, glucose oxidation, and glycogen synthesis in isolated rat hepatocytes

1989 ◽  
Vol 67 (10) ◽  
pp. 724-729 ◽  
Author(s):  
Francesc Moreno ◽  
Marcal Pastor-Anglada ◽  
Morley D. Hollenberg ◽  
Maria Soley
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Glycogen Synthesis ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Maximal Stimulation ◽  
Epidermal Growth ◽  
Glucagon And Insulin ◽  
Oxidation Of Glucose ◽  
Isolated Rat

Using isolated rat hepatocytes, we studied the effect of epidermal growth factor (urogastrone) (EGF-URO) on the incorporation of [3-14C]pyruvate into glucose and glycogen, on the incorporation of [U-14C]glucose into glycogen, and on the oxidation of [U-14C]glucose to 14CO2. The effects of EGF-URO were compared with those of glucagon and insulin. EGF-URO, with an EC50 of 0.2 nM, enhanced by 34% (maximal stimulation) the conversion of [3-14C]pyruvate into glucose; no effect was observed on the oxidation of glucose to CO2 and on the incorporation of either pyruvate or glucose into glycogen. The effect of EGF-URO on pyruvate conversion to glucose was observed only when hepatocytes were preincubated with EGF-URO for 40 min prior to the addition of substrate. Glucagon (10 nM) increased the incorporation of [3-14C]pyruvate into glucose (44% above control); however, unlike EGF-URO, glucagon stimulated gluconeogenesis better without than with a preincubation period. Neither insulin nor EGF-URO (both 10 nM) affected the incorporation of [U-14C]glucose into glycogen during a 20-min incubation period. However, at longer time periods of incubation with the substrate (60 instead 20 min), insulin (but not EGF-URO) increased the incorporation of [14C]glucose into glycogen; EGF-URO counteracted this stimulatory effect of insulin. In contrast with previous data, our work indicates that EGF-URO can, under certain conditions, counteract the effects of insulin and, like glucagon, promote gluconeogenesis in isolated rat hepatocytes.Key words: rat hepatocytes, EGF-URO, gluconeogenesis, glycogen synthesis.

Effects of in vivo and in vitro Alcohol Administration on Insulin Binding and Glycogenesis in Isolated Rat Hepatocytes

1983 ◽  
Vol 27 (4) ◽  
pp. 313-319 ◽  
Author(s):  
R.M. Rifkin ◽  
W.W. Todd ◽  
D.R. Toothaker ◽  
A. Sussman ◽  
M. Trowbridge ◽  
...  
Keyword(s):  
Rat Hepatocytes ◽  
Insulin Binding ◽  
Isolated Rat Hepatocytes ◽  
Alcohol Administration ◽  
Isolated Rat

Comparison of 125-I-insulin binding and degradation to isolated rat hepatocytes and liver membranes

Diabetes ◽  
1975 ◽  
Vol 24 (9) ◽  
pp. 801-810 ◽  
Author(s):  
J. Olefsky ◽  
J. Johnson ◽  
F. Liu ◽  
P. Edwards ◽  
S. Baur
Keyword(s):  
Rat Hepatocytes ◽  
Insulin Binding ◽  
Isolated Rat Hepatocytes ◽  
Liver Membranes ◽  
Isolated Rat

The effects of colchicine on insulin binding to isolated rat hepatocytes

1981 ◽  
Vol 9 (1) ◽  
pp. 88-89
Author(s):  
JONATHAN WHITTAKER ◽  
VANESSA A. HAMMOND ◽  
K. GEORGE ◽  
M. M. ALBERTI
Keyword(s):  
Rat Hepatocytes ◽  
Insulin Binding ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat
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