Construction of Beta procumbens-specific DNA probes and their application for the screening of B. vulgaris x B. procumbens (2n = 19) addition lines

1990 ◽  
Vol 79 (2) ◽  
pp. 177-181 ◽  
Author(s):  
T. Schmidt ◽  
H. Junghans ◽  
M. Metzlaff
Keyword(s):  
Dna Probes ◽  
Addition Lines ◽  
Beta Procumbens

Assignment of the genomic affinities of chromosomes from polyploid Elymus species added to wheat

Genome ◽  
1988 ◽  
Vol 30 (1) ◽  
pp. 70-82 ◽  
Author(s):  
Bikram S. Gill ◽  
Kay L. D. Morris ◽  
Rudi Appels
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Single Gene ◽  
Dna Probes ◽  
Addition Lines ◽  
Cross Hybridization ◽  
Elymus Trachycaulus ◽  
Chinese Spring Wheat ◽  
5S Dna ◽  
Elymus Species

Genomic DNAs from 10 disomic addition, substitution, or translocation chromosomes from tetraploid Elymus trachycaulus (SSHH) and 13 tetraploid Elymus ciliaris (SSYY) in Chinese Spring wheat were assayed with 18S–28S rDNA (Nor), 5S DNA, Adh, α-amylase, β-glucanase gene clones, and S-genome and H-genome repetitive DNA sequences. The rDNA from the S genome and 5S DNA from the S and H genomes, under high stringency Southern blot analysis, distinguished S-genome and H-genome loci on individual Elymus chromosomes. The single gene, or low copy gene probes (Adh and others), allowed identification of different addition lines and provided information on the gene synteny relationships of Elymus chromosomes with wheat chromosomes. The S-genome specific and H-genome repetitive DNA probes were not useful in assigning genomic affinities, due to some cross-hybridization between these genomes using these probes and (or) the occurrence of large numbers of translocations in polyploid genomes of Elymus species. However, the repetitive DNA probes provided diagnostic phenotypic markers for identification of individual Elymus addition lines. Moreover, since S- and H-genome DNA sequences were virtually absent from wheat, they were excellent markers for the detection of Elymus chromatin in wheat. The array of DNA probes should prove useful in chromosome manipulation in resistance transfer from Elymus into wheat.Key words: Triticeae, Elymus, aneuploid, DNA probe.

Registration of Six Wheat-Rye Addition Lines Resistant to the Russian Wheat Aphid

2011 ◽  
Vol 5 (3) ◽  
pp. 426-429 ◽  
Author(s):  
Kabwe K. Nkongolo ◽  
Scott D. Haley ◽  
James S. Quick ◽  
Frank B. Peairs
Keyword(s):  
Russian Wheat Aphid ◽  
Addition Lines

Development of pattern recognition based on the nanosheet-DNA probes and an extendible DNA library

The Analyst ◽  
2021 ◽  
Author(s):  
Jiawei Qi ◽  
Pinhua Rao ◽  
Lele Wang ◽  
Li Xu ◽  
Yanli Wen ◽  
...  
Keyword(s):  
Pattern Recognition ◽  
High Throughput ◽  
Analysis Data ◽  
Dna Probes ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Dna Library ◽  
Array Sensing

Pattern recognition, also called “array sensing” is a recognition strategy with a wide and expandable analysis range, based on the high-throughput analysis data. In this work, we constructed a sensor...

scholarly journals Production and Characterization of Maize Chromosome 9 Radiation Hybrids Derived From an Oat-Maize Addition Line

Genetics ◽  
2000 ◽  
Vol 156 (1) ◽  
pp. 327-339 ◽  
Author(s):  
O Riera-Lizarazu ◽  
M I Vales ◽  
E V Ananiev ◽  
H W Rines ◽  
R L Phillips
Keyword(s):  
Radiation Hybrid ◽  
Chromosome Region ◽  
Chromosome 9 ◽  
Addition Line ◽  
Addition Lines ◽  
Organization Studies ◽  
Maize Chromosome ◽  
Chromosome Addition ◽  
Radiation Hybrids ◽  
Chromosome Addition Lines

Abstract In maize (Zea mays L., 2n = 2x = 20), map-based cloning and genome organization studies are often complicated because of the complexity of the genome. Maize chromosome addition lines of hexaploid cultivated oat (Avena sativa L., 2n = 6x = 42), where maize chromosomes can be individually manipulated, represent unique materials for maize genome analysis. Maize chromosome addition lines are particularly suitable for the dissection of a single maize chromosome using radiation because cultivated oat is an allohexaploid in which multiple copies of the oat basic genome provide buffering to chromosomal aberrations and other mutations. Irradiation (gamma rays at 30, 40, and 50 krad) of a monosomic maize chromosome 9 addition line produced maize chromosome 9 radiation hybrids (M9RHs)—oat lines possessing different fragments of maize chromosome 9 including intergenomic translocations and modified maize addition chromosomes with internal and terminal deletions. M9RHs with 1 to 10 radiation-induced breaks per chromosome were identified. We estimated that a panel of 100 informative M9RHs (with an average of 3 breaks per chromosome) would allow mapping at the 0.5- to 1.0-Mb level of resolution. Because mapping with maize chromosome addition lines and radiation hybrid derivatives involves assays for the presence or absence of a given marker, monomorphic markers can be quickly and efficiently mapped to a chromosome region. Radiation hybrid derivatives also represent sources of region-specific DNA for cloning of genes or DNA markers.

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