Electron microscopic and enzyme cytochemical studies on the guinea pig metaphysis with special reference to the lysosomal system of different cell types

1975 ◽  
Vol 156 (3) ◽  
Author(s):  
Johan Thyberg ◽  
Stefan Nilsson ◽  
Ulf Friberg
1985 ◽  
Vol 101 (4) ◽  
pp. 1442-1454 ◽  
Author(s):  
P Cowin ◽  
H P Kapprell ◽  
W W Franke

Desmosomal plaque proteins have been identified in immunoblotting and immunolocalization experiments on a wide range of cell types from several species, using a panel of monoclonal murine antibodies to desmoplakins I and II and a guinea pig antiserum to desmosomal band 5 protein. Specifically, we have taken advantage of the fact that certain antibodies react with both desmoplakins I and II, whereas others react only with desmoplakin I, indicating that desmoplakin I contains unique regions not present on the closely related desmoplakin II. While some of these antibodies recognize epitopes conserved between chick and man, others display a narrow species specificity. The results show that proteins whose size, charge, and biochemical behavior are very similar to those of desmoplakin I and band 5 protein of cow snout epidermis are present in all desmosomes examined. These include examples of simple and pseudostratified epithelia and myocardial tissue, in addition to those of stratified epithelia. In contrast, in immunoblotting experiments, we have detected desmoplakin II only among cells of stratified and pseudostratified epithelial tissues. This suggests that the desmosomal plaque structure varies in its complement of polypeptides in a cell-type specific manner. We conclude that the obligatory desmosomal plaque proteins, desmoplakin I and band 5 protein, are expressed in a coordinate fashion but independently from other differentiation programs of expression such as those specific for either epithelial or cardiac cells.


1975 ◽  
Vol 21 (2) ◽  
pp. 121-129 ◽  
Author(s):  
J. G. Zeikus ◽  
V. G. Bowen

Electron-microscopic studies using thin sections revealed that methane-producing bacteria were an ultrastructurally diverse group. Fine structure and morphological characteristics separated these bacteria into four discrete cell types. Methanogenic bacteria displayed a gram-positive cell wall that varied considerably among different cell types. Differences in granular inclusions, reserve materials, and intracytoplasmic membranes were observed. Unique ultrastructural features were not shared by all methanogenic species studied.


Author(s):  
Toshiyuki Ishiwata ◽  
Fumio Hasegawa ◽  
Masaki Michishita ◽  
Norihiko Sasaki ◽  
Naoshi Ishikawa ◽  
...  

1968 ◽  
Vol 5 (5) ◽  
pp. 451-470 ◽  
Author(s):  
Per H. J. Nafstad ◽  
Inger Nafstad

The ultrastructure of blood and bone marrow cells in the normal pig was studied; the process of maturation of the different cell types was found to be essentially in accordance with that in other animal species. The eosinophilic granules had a pattern which differed from that in other mammals, being characterized by specific internal structure in the immature stages. During maturation, however, a homogenous appearance supervened. Moreover, the lymphocytic nuclei were found to have irregular shapes as compared with the light microscopic picture. The results are compared to some reported studies of other animal species.


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