A RFLP linkage map of Sorghum bicolor (L.) Moench

1994 ◽  
Vol 89-89 (2-3) ◽  
pp. 139-145 ◽  
Author(s):  
G. -W. Xu ◽  
C. W. Magill ◽  
K. F. Schertz ◽  
G. E. Hart
Keyword(s):  
Sorghum Bicolor ◽  
Linkage Map ◽  
Sorghum Bicolor L

An integrated SSR and RFLP linkage map of Sorghum bicolor (L.) Moench

Genome ◽  
2000 ◽  
Vol 43 (6) ◽  
pp. 988-1002 ◽  
Author(s):  
Dinakar Bhattramakki ◽  
Jianmin Dong ◽  
Ashok K Chhabra ◽  
Gary E Hart
Keyword(s):  
Sorghum Bicolor ◽  
Linkage Map ◽  
Dna Sequences ◽  
Artificial Chromosome ◽  
Polymorphic Loci ◽  
Dna Libraries ◽  
Ssr Loci ◽  
Bac Libraries ◽  
Primer Sets ◽  
Sorghum Bicolor L

We report the development, testing, and use (for genetic mapping) of a large number of polymerase chain reaction (PCR) primer sets that amplify DNA simple sequence repeat (SSR) loci of Sorghum bicolor (L.) Moench. Most of the primer sets were developed from clones isolated from two sorghum bacterial artificial chromosome (BAC) libraries and three enriched sorghum genomic-DNA (gDNA) libraries. A few were developed from sorghum DNA sequences present in public databases. The libraries were probed with radiolabeled di- and trinucleotide oligomers, the BAC libraries with four and six oligomers, respectively, and the enriched gDNA libraries with four and three oligomers, respectively. Both types of libraries were markedly enriched for SSRs relative to a size-fractionated gDNA library studied earlier. However, only 2% of the sequenced clones obtained from the size-fractionated gDNA library lacked a SSR, whereas 13% and 17% of the sequenced clones obtained from the BAC and enriched gDNA libraries, respectively, lacked a SSR. Primer sets were produced for 313 SSR loci. Two-hundred sixty-six (85%) of the loci were amplified and 165 (53%) of the loci were found to be polymorphic in a population composed of 18 diverse sorghum lines. (AG/TC)n and (AC/TG)n repeats comprised 91% of the dinucleotide SSRs and 52% of all of the SSRs at the polymorphic loci, whereas four types of repeats comprised 66% of the trinucleotide SSRs at the loci. Primer sequences are reported for the 165 polymorphic loci and for eight monomorphic loci that have a high degree of homology to genes. Also reported are the genetic map locations of 113 novel SSR loci (including four SSR-containing gene loci) and a linkage map composed of 147 SSR loci and 323 RFLP (restriction fragment length polymorphism) loci. The number of SSR loci per linkage group ranges from 8 to 30. The SSR loci are distributed relatively evenly throughout approximately 75% of the 1406-cM linkage map, but segments of five linkage groups comprising about 25% of the map either lack or contain few SSR loci. Mapping of SSR loci isolated from BAC clones located to these segments is likely to be the most efficient method for placing SSR loci in the segments.Key words: DNA libraries, linkage mapping, Sorghum bicolor, SSRs.

scholarly journals SATURATION OF STRIGA RESISTANCE QTLS IN SORGHUM (Sorghum bicolor (L.) MOENCH)

2016 ◽  
Vol 1 (1) ◽  
pp. 43
Author(s):  
Rasha Ali ◽  
Abdalla H. Mohamed ◽  
Adil Elhussein ◽  
Charles T. Hash
Keyword(s):  
Sorghum Bicolor ◽  
Linkage Map ◽  
Marker Assisted Selection ◽  
Genetic Linkage ◽  
Control Measures ◽  
Striga Hermonthica ◽  
Resistance Qtls ◽  
Striga Resistance ◽  
Genomic Regions ◽  
Sorghum Bicolor L

Sorghum bicolor (L.) Moench is the 5th most important cereal crop worldwide. The main biotic constraint to sorghum production is the parasitic weed Striga hermonthica (Del.) Benth. Striga is controlled using a combination of cultural, chemical and bio-control measures which cannot be afforded by farmers. A cost-effective alternative is to breed varieties resistant to Striga. Previous studies involving development of a sorghum genetic linkage map and mapping genomic regions contributing to Striga resistance have shown that resistance is a complex trait controlled by at least five QTLs. Genetic linkage maps provide an important genomic resource for understanding genome organization and evolution, and genetic basis of quantitative traits, and provide useful information for identifying and isolating genes responsible for a given phenotype. Therefore there is need for identification of molecular markers closely linked to these resistance QTLs to improve efficiency of marker-assisted selection (MAS) to accelerate development of Striga-resistant varieties. The aim of this study was to saturate genomic regions of Striga resistance QTLs using SSRs and DArT markers. QTL regions associated with Striga resistance were well saturated and confidence intervals for these QTLs were reduced: 27 SSR markers, two morphological markers, along with 175 DArTs markers were added to the previously mapped skeleton linkage map on linkage groups SBI-01, SBI-02, SBI-05a, SBI-05b and SBI-06 at intervals of 3-5 cM. Identified markers would be useful in marker-assisted selection for Introgression of this trait into susceptible sorghum cultivars. Addition of markers tightly linked to Striga resistance QTLs are not only advantageous for MAS application, but also assisted in saturating the sorghum linkage map.

Consórcio palma-sorgo sob lâminas de irrigação: balanço de água no solo e coeficientes da cultura

Agrometeoros ◽  
2020 ◽  
Vol 27 (2) ◽  
Author(s):  
Cleber Pereira Alves ◽  
Thieres George Freire Silva ◽  
Hygor Kristoph Muniz Nunes Alves ◽  
Alexandre Maniçoba da Rosa Ferraz Jardim ◽  
Luciana Sandra Bastos de Souza ◽  
...  
Keyword(s):  
Sorghum Bicolor ◽  
Sorghum Bicolor L

Objetivou-se neste estudo quantificar a evapotranspiração real (ETr) e máxima da cultura (ETc) e os coeficientes da cultura (Kc) do consórcio palma-sorgo. O experimento foi conduzido no município de Serra Talhada, PE. O delineamento usado foi em blocos ao acaso, envolvendo cinco lâminas de irrigação (0, 25, 50, 75 e 100% da evapotranspiração de referência - ETo), sob sistema de cultivo consorciado palma-sorgo. O clone de palma forrageira utilizado foi a Orelha de Elefante Mexicana (Opuntia stricta (Haw.) Haw.) e o cultivar de sorgo, Sorghum bicolor (L.) Moench, a SF 15. O sorgo foi conduzido durante dois ciclos (planta e rebrota) compreendidos em um ciclo anual da palma. A quantificação da ETr e da ETc foi realizada através do resíduo do balanço de água no solo (BAS) a cada 14 dias, com a mensuração dos componentes hidrodinâmicos. As determinações da ETc e do Kc foram realizadas com base na lâmina de 75% da ETo. Os componentes do BAS foram submetidos à análise de regressão, sendo testados modelos polinomiais. Com exceção da variação do armazenamento de água no solo, os demais componentes hidrodinâmicos do solo cultivado sob sistema consorciado palma-sorgo respondem linearmente ao aumento de lâminas de irrigação. A evapotranspiração média diária do consórcio palma-sorgo é igual a 3,0 mm dia-1, independentemente da lâmina de irrigação. Os coeficientes do consórcio palma-sorgo são iguais a 0,40, 0,68, 0,90 e 0,52 durante as fases I, II, III e IV de emissão de cladódios.

Effet antioxydant et antihyperglycémiant du seigle (Secale cereale L.) et du sorgho (Sorghum bicolor L.) chez le rat Wistar rendu diabétique

2018 ◽  
Vol 16 (S1) ◽  
pp. S273-S283
Author(s):  
Z. Mami-Soualem ◽  
N. Brixi ◽  
C. Beghdad ◽  
M. Belarbi
Keyword(s):  
Sorghum Bicolor ◽  
Secale Cereale ◽  
Stress Oxydant ◽  
Habitudes Alimentaires ◽  
Diabète Sucré ◽  
Secale Cereale L ◽  
Vitamine C ◽  
Fibres Alimentaires ◽  
Peroxydation Lipidique ◽  
Sorghum Bicolor L

Le changement des habitudes alimentaires au cours de ces dernières années a abouti à l’apparition des maladies dites de civilisation, parmi elles le diabète sucré. Des approches diétothérapeutiques privilégient la consommation des produits céréaliers sous la forme la plus complète possible. Ce travail a pour but de tester l’efficacité des régimes expérimentaux préparés à base des grains complets des deux céréales, le sorgho (Sorghum bicolor L.) et le seigle (Secale cereale L.), sur la correction de l’hyperglycémie et des marqueurs du stress oxydant associés au diabète chez le rat mâle Wistar. Les résultats montrent que les rats diabétiques, soumis au régime seigle pendant quatre semaines, présentent une diminution significative de la glycémie qui atteint les 53,95 % à la fin de l’expérimentation, ce qui fait que leur taux en glycémie se rapproche de celui des normoglycémiques. Le sorgho blanc présente, d’une part, une diminution significative de la peroxydation lipidique intracellulaire et, d’autre part, une augmentation de l’activité de la glutathion peroxydase, la glutathion réductase ainsi que la vitamine C. Ces approches méthodologiques peuvent, en améliorant la connaissance de l’importance des céréales (fibres alimentaires solubles et des polyphénols) dans l’évolution du diabète, aboutir à des recommandations et à une éducation nutritionnelle du diabétique.

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