Quantitative morphological study of smooth muscle cells of the guinea-pig taenia coli

1976 ◽  
Vol 170 (2) ◽  
Author(s):  
Giorgio Gabella
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Morphological Study ◽  
Muscle Cells ◽  
Taenia Coli

scholarly journals Effect of DFP on loading of fura-2/AM into single smooth muscle cells prepared from guinea pig taenia coli

1987 ◽  
Vol 43 ◽  
pp. 240
Author(s):  
Hideto Oyamada ◽  
Ikuo Maruyama ◽  
Tomohiko Hasegawa ◽  
Kazuko Otsuka ◽  
Kazutaka Momose
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Taenia Coli ◽  
Fura 2

Preparation of single smooth muscle cells from guinea pig taenia coli by combinations of purified collagenase and papain

1987 ◽  
Vol 18 (2) ◽  
pp. 151-161 ◽  
Author(s):  
Maruyama Ikuo ◽  
Yoshida Chieko ◽  
Kobayashi Mamoru ◽  
Oyamada Hideto ◽  
Momose Kazutaka
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Taenia Coli

scholarly journals The Ca2+-activated K+ channel and its functional roles in smooth muscle cells of guinea pig taenia coli.

1989 ◽  
Vol 94 (5) ◽  
pp. 833-847 ◽  
Author(s):  
S L Hu ◽  
Y Yamamoto ◽  
C Y Kao
Keyword(s):  
Smooth Muscle ◽  
Membrane Potential ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Resting Potential ◽  
K Channel ◽  
Taenia Coli ◽  
Open Probability ◽  
Open Time

Currents through single potassium channels were studied in cell-attached or inside-out patches from collagenase-dispersed smooth muscle cells of the guinea pig taenia coli. Under conditions mimicking the physiological state with [K+]i = 135 mM: [K+]o = 5.4 mM, three distinct types of K+ channel were identified with conductances around 0 mV of 147, 94, and 63 pS. The activities of the 94- and 63-pS channel were observed infrequently. The 147-pS channel was most abundant. It has a reversal potential of approximately -75 mV. It is sensitive to [Ca2+]i and to membrane potential. At -30 mV, the probability of a channel being open is at a minimum. At more positive voltages, the probability follows Boltzman distribution. A 10-fold change in [Ca2+]i causes a 25-mV negative shift of the voltage where half of the channels are open; an 11.3-mV change in membrane potential produces an e-fold increase in the probability of the channel being open when P is low. At voltages between -30 and -50 mV, the open probability increases in an anomalous manner because of a large decrease of the channel closed time without much change in the channel open time. This anomalous activity may play a regulatory role in maintaining the resting potential. The histograms of channel open and closed time fit well, respectively, with single and double exponential distributions. Upon step depolarizations by 100-ms pulses, the 147-pS channel opens with a brief delay. The delay shortens and both the number of open channels and the open time increase with increasing positivity of the potential. The averaged currents during the step depolarizations closely resemble the delayed rectifying outward K+ currents in whole-cell recordings.

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