Pollination in vitro: effects on the growth of pollen tubes, seed set and gametophytic self-incompatibility in Trifolium pratense L. and T. repens L.

1990 ◽  
Vol 80 (5) ◽  
pp. 657-664 ◽  
Author(s):  
N. Leduc ◽  
G. C. Douglas ◽  
M. Monnier ◽  
V. Connolly
Keyword(s):  
Trifolium Pratense ◽  
Seed Set ◽  
Pollen Tubes ◽  
Self Incompatibility ◽  
Trifolium Pratense L ◽  
In Vitro Effects

scholarly journals Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes.

1991 ◽  
pp. 271-283 ◽  
Author(s):  
J. E. Gray ◽  
B. A. McClure ◽  
I. Bonig ◽  
M. A. Anderson ◽  
A. E. Clarke
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Nicotiana Alata ◽  
Self Incompatibility

Breeding Systems of Disjunct Populations of Christmas Bells (Blandfordia grandiflora R.Br., Liliaceae): Variation in Self-Fertility and an Ovular Mechanism Regulating Self-Fertilisation

1993 ◽  
Vol 41 (1) ◽  
pp. 35 ◽  
Author(s):  
M Ramsey ◽  
N Prakash ◽  
S Cairns
Keyword(s):  
Seed Set ◽  
Pollen Tubes ◽  
Breeding Systems ◽  
Seed Abortion ◽  
Self Incompatibility ◽  
Coastal Plants ◽  
Hand Pollination ◽  
Disjunct Populations ◽  
Pollen Vectors ◽  
Blandfordia Grandiflora

The breeding systems of disjunct tableland and coastal populations of Christmas bells were determined using hand-pollination experiments. In both populations, 90% or more of self-pollinated plants produced seeds. Tableland plants were significantly more self-fertile than coastal plants (ratio of self seed set to cross seed set: tableland, 0.55 ± 0.03; coast, 0.08 ± 0.02). Significant variation among plants for self-fertility was found in both populations. Autofertility was 1.6% or less in both populations indicating that pollen vectors are necessary for seed set. Seed set by agamospermy in both populations was less than 0.1%. Percentage seed abortion was greater in self-pollinated plants than cross-pollinated plants in both populations. In both self- and cross-pollinated plants, seed abortion was twice as great in the coastal population than in the tableland population. No evidence was found for stigmatic or stylar self-incompatibility. Self and cross pollen adhered to and germinated equally well on stigmas in both populations (72 - 77% germination). Similarly, there were no differences between pollination treatments or populations in the percentage of ovules penetrated by pollen tubes (82 - 89% penetration). When self-pollination preceded cross-pollination by 24 h or longer seed set was significantly reduced compared to flowers that were cross-pollinated only, suggesting ovules were pre-empted by self pollen tubes. Collectively these results strongly suggest that self seed set was reduced by a mechanism operating at the ovule level, such as early-acting inbreeding depression due to recessive seed-aborting genes, although incomplete late-acting self-incompatibility cannot be ruled out. For coastal plants, this ovular mechanism largely prevents selfing, indicating plants were predominantly outcrossing although most produced some self seed. For tableland plants, substantial seed set by selfing may occur under natural conditions.

scholarly journals The Role of Amino Acids for the In Vitro Culture of Some Species of Forage Legumes I. Trifolium pratense L

2013 ◽  
Vol 70 (1) ◽  
pp. 87-92
Author(s):  
Gabriela Maria VICAȘ ◽  
Mircea SAVATTI
Keyword(s):  
Amino Acids ◽  
In Vitro Culture ◽  
Culture Medium ◽  
Trifolium Pratense ◽  
Basal Medium ◽  
Red Clover ◽  
Forage Legumes ◽  
Trifolium Pratense L

Establishing the effect of the amino acids as additional additives to the culture medium is and will be in the future one of our concerns of interest for the in vitro culture of some plants. The present study examines the effect of the glicocol added to the LS basal medium over the embryos of the Trifolium pratense L specie cultivated in vitro. There were followed: the percentage of plant regeneration of the red clover, its multiplication capacity and the formation of the root system, and also the evolution of the callus obtained on mediums with 2,4D, BA and amino acid.

scholarly journals Self-incompatibility in Cornus florida

HortScience ◽  
2004 ◽  
Vol 39 (2) ◽  
pp. 335-338 ◽  
Author(s):  
Sandra M. Reed
Keyword(s):  
Growth Rate ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Seed Set ◽  
Pollen Tubes ◽  
Cornus Florida ◽  
Self Incompatibility ◽  
Flowering Dogwood ◽  
Pollen Tube Growth Rate ◽  
Upper Third

Low seed set has been reported following self-pollinations of flowering dogwood (Cornus florida L.). The objective of this study was to verify the presence of self-incompatibility in C. florida. `Cherokee Princess' stigmas and styles were collected 1, 2, 4, 8, 12, 24, 48, and 72 hours after cross- and self-pollinations, stained with aniline blue and observed using a fluorescence microscope. Pollen germinated freely following self-pollinations, but self-pollen tubes grew slower than those resulting from cross-pollinations. By 48 hours after cross-pollination, pollen tubes had reached the bottom of the style while pollen tubes in self-pollinated flowers had only penetrated the upper third of the style. Evidence of reduced pollen tube growth rate in self-pollinations of `Cherokee Chief' and `Cherokee Brave' was also obtained. This study provides evidence of a gametophytic self-incompatibity system in C. florida. It was also determined that stigmas of C. florida `Cherokee Princess' are receptive to pollen from 1 day prior to anthesis to 1 day after anthesis.

scholarly journals Seed Production of Red Clover (Trifolium pratense L.) under Danish Field Conditions

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1289
Author(s):  
Shuxuan Jing ◽  
Birte Boelt
Keyword(s):  
Seed Production ◽  
Seed Yield ◽  
Seed Weight ◽  
Trifolium Pratense ◽  
Seed Set ◽  
Red Clover ◽  
Flower Head ◽  
Trifolium Pratense L ◽  
Thousand Seed Weight ◽  
High Seed Yield

High and stable seed yield is critical for red clover (Trifolium pratense L.) seed production and the commercial exploitation of the crop. A three-year experiment was conducted from 2013 to 2015 under Danish field conditions to explore the influence of precipitation during peak flowering on the seed yield of three red clover cultivars. We investigated the flowering duration and intensity based on a visual scale assessment, seed yield, and thousand seed weight in all three experimental years. In 2014 and 2015 we measured the seed yield components of floret number per flower head, seed number per flower head, and seed set. During the experimental period, high seed yields of more than 1000 kg ha−1 were obtained for the diploid cultivars ‘Rajah’ and ‘Suez’. Although a relatively high seed yield of 500 kg ha−1 was obtained in the tetraploid cultivar ‘Amos’, this was only around half of the seed yield and seed set of the diploid cultivars. Precipitation during peak flowering positively influenced the seed yield and thousand seed weight for the three cultivars. We conclude that observations of flowering phenology are required to determine the impact of environmental conditions on seed yield in red clover cultivars. Further, adequate water supply during peak flowering is important to obtain the high seed yield of red clover.

Isoflavone content and estrogenic activity of different batches of red clover (Trifolium pratense L.) extracts: An in vitro study in MCF-7 cells

Fitoterapia ◽  
2014 ◽  
Vol 94 ◽  
pp. 62-69 ◽  
Author(s):  
Paola Spagnuolo ◽  
Emanuela Rasini ◽  
Alessandra Luini ◽  
Massimiliano Legnaro ◽  
Marcello Luzzani ◽  
...  
Keyword(s):  
Trifolium Pratense ◽  
Estrogenic Activity ◽  
Red Clover ◽  
In Vitro Study ◽  
Vitro Study ◽  
Isoflavone Content ◽  
Trifolium Pratense L ◽  
Mcf 7
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