The identification of the kappa-casein genotype in Holstein dairy cattle using the polymerase chain reaction

1990 ◽  
Vol 80 (5) ◽  
pp. 631-634 ◽  
Author(s):  
D. Zadworny ◽  
U. Kuhnlein
Keyword(s):  
Polymerase Chain Reaction ◽  
Dairy Cattle ◽  
Chain Reaction ◽  
Polymerase Chain

An evaluation of melarsomine hydrochloride efficacy for parasitological cure in experimental infection of dairy cattle with Trypanosoma evansi in Thailand

Parasitology ◽  
2011 ◽  
Vol 138 (9) ◽  
pp. 1134-1142 ◽  
Author(s):  
MARC DESQUESNES ◽  
KETSARIN KAMYINGKIRD ◽  
TIMOTHÉE VERGNE ◽  
NACHAI SARATAPHAN ◽  
RODTIAN PRANEE ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Dairy Cattle ◽  
Experimental Infection ◽  
Intramuscular Injection ◽  
Trypanosoma Evansi ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chain Reaction ◽  
Polymerase Chain

SUMMARYMelarsomine hydrochloride can cure Trypanosoma evansi infection in camels at a dose of 0·25 mg/kg, but at that dose relapses occur in cattle. In our study, the efficacy of an intramuscular injection of melarsomine hydrochloride at 0·5 mg/kg was assessed in 3 normal and 3 splenectomized dairy cattle experimentally infected with a stock of T. evansi from Thailand. The animals were monitored for 5 months by haematocrit centrifugation, blood- or cerebrospinal fluid-mouse inoculation, polymerase chain reaction, the card agglutination test (CATT) for T. evansi, and the enzyme-linked immunosorbent assay‑T. evansi. Parasitological and DNA tests became and remained negative just after treatment. By the end of the experiment, CATT was negative and ELISA scores were below or very close to the cut-off value. One of the splenectomized cattle died from anaplasmosis during the experiment, but tested negative for surra. It was concluded that the parasites had been cleared from the cattle, and melarsomine hydrochloride at 0·5 mg/kg can be recommended for treatment against T. evansi infection in dairy cattle in Thailand. Further work is necessary to validate the efficacy of the treatment in the event of confirmed CSF-infection.

Diagnosis of dermatophilosis in dairy cattle in Kerala, India

2015 ◽  
Author(s):  
P. V. Tresamol ◽  
M. R. Saseendranath
Keyword(s):  
Polymerase Chain Reaction ◽  
16S Rrna ◽  
Dairy Cattle ◽  
Anaerobic Condition ◽  
Microscopical Examination ◽  
Chain Reaction ◽  
Molecular Studies ◽  
Experimental Inoculation ◽  
Polymerase Chain ◽  
Specific Band

Skin scabs and scrapings from 82 dermatitis cases in dairy cattle were subjected to detailed bacteriological, mycological, parasitological and molecular studies. Microscopical examination of Giemsa or Gram’s stained smears of scab material from the lesions revealed characteristic gram positive septate branching filaments with typical tram track appearance suggestive of Dermatophilus congolensis in 72 samples (91.5%). Culture of scab materials in sheep blood agar under anaerobic condition yielded typical beta haemolytic colonies of D. congolensis in 75 samples, which were further confirmed by colony morphology, staining characters and biochemical reactions. Molecular confirmation of the isolates was carried out using polymerase chain reaction with primers based on 16S rRNA which yielded specific band of 500bp. The pathogenicity of the isolates was also proved by experimental inoculation into rabbits.

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