Analysis of molecular markers associated with powdery mildew resistance genes in peach (Prunus persica (L.) Batsch)xPrunus davidiana hybrids

1996 ◽  
Vol 93-93 (5-6) ◽  
pp. 909-919 ◽  
Author(s):  
E. Dirlewanger ◽  
T. Pascal ◽  
C. Zuger ◽  
J. Kervella
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Resistance Genes ◽  
Prunus Persica ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance

scholarly journals Identification of apple scab and powdery mildew resistance genes in Czech apple (Malus × domestica) genetic resources by PCR molecular markers

2011 ◽  
Vol 47 (No. 4) ◽  
pp. 156-165 ◽  
Author(s):  
J. Patzak ◽  
F. Paprštein ◽  
A. Henychová
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Genetic Resources ◽  
Resistance Genes ◽  
Powdery Mildew Resistance ◽  
Venturia Inaequalis ◽  
Apple Scab ◽  
Scab Resistance ◽  
Mildew Resistance ◽  
Apple Cultivars

The presence of genes for resistance to scab (Venturia inaequalis) and powdery mildew (Podosphaera leucotricha) was studied using molecular markers in a sample of 279 apple cultivars from the Czech collection of apple genetic resources. The sample comprised 37 cultivars supposed to have the Vf gene for scab resistance, 97 reference world cultivars and 145 old and local cultivars. Six PCR molecular markers for the scab resistance genes Vf, Vm, Vbj, Vr and Vh and three PCR molecular markers for the powdery mildew resistance genes Pl-w, Pl-1 and Pl-d were used. The marker for the major scab resistance gene Vf was detected in all cultivars supposed to have Vf, except in Romus 1, and in the three small-fruited cultivars Malus Evereste, Golden Gem and Hilleri. The markers of the Vr and Vh scab resistance genes were detected in 22 cultivars in combination with the marker for Vf, in 56 reference world cultivars and in 82 old and local apple cultivars. PCR molecular markers for one or two of the powdery mildew resistance genes were detected in the small-fruited cultivars Malus Evereste, Golden Gem, prof. Sprengeri and Hilleri; and in the larger fruited cultivars Hagloe Crab, Borovinka and Tita Zetei. We did not find markers for the scab resistance genes Vm and Vbj in any of the studied cultivars. They are absent also in the remaining part of the Czech collection of apple genetic resources. PCR molecular markers are useful tools for the identification of resistance genes within apple germplasm collections and can be used to increase the number of sources for disease resistance in breeding programmes.

scholarly journals Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii

2019 ◽  
Author(s):  
Zhenjie Dong ◽  
Xiubin Tian ◽  
Chao Ma ◽  
Qing Xia ◽  
Beilin Wang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Binding Site ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Powdery Mildew Resistance ◽  
Nucleotide Binding ◽  
Leucine Rich Repeat ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance

Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is one of many severe diseases that threaten bread wheat (Triticum aestivum L.) yield and quality worldwide. The discovery and deployment of powdery mildew resistance genes (Pm) can prevent this disease epidemic in wheat. In a previous study, we transferred the powdery mildew resistance gene Pm57 from Aegilops searsii into common wheat and cytogenetically mapped the gene in a chromosome region with the fraction length (FL) 0.75-0.87, which represents 12% of 2Ss#1 segment on the long arm of chromosome 2Ss#1. In this study, we performed RNA-Seq on three infected and mock-infected wheat-Ae. searsii 2Ss#1 introgression lines with Bgt-isolates inoculation at 0, 12, 24, and 48 hours after inoculation. Then we designed 79 molecular markers based on transcriptome sequences and physically mapped them to Ae. searsii chromosome 2Ss#1- in seven intervals. We used these markers to identify 46 wheat-Ae. searsii 2Ss#1 recombinants induced by ph1b, a deletion mutant of pairing homoelogous (Ph) genes. Analysis of the 46 ph1b-induced 2Ss#1L recombinants with different Bgt-responses using 28 2Ss#1L-specific molecular markers in the interval FL0.72-0.87 where Pm57 is located, and the flanking intervals, we physically mapped Pm57 gene on the long arm of 2Ss#1 in a 5.13 Mb genomic region, which was flanked by markers X67593 (773.72 Mb) and X62492 (778.85 Mb). By comparative synteny analysis of the corresponding region on chromosome 2B in Chinese spring (T. aestivum L.) with other model species we identified ten genes that are putative plant defense-related (R) genes which includes six coiled-coil nucleotide-binding site-leucine-rich repeat (CNL), three nucleotide-binding site-leucine-rich repeat (NL) and a leucine-rich receptor-like repeat (RLP) encoding proteins. This study will lay a foundation for further cloning of Pm57, and benefit the understanding of interactions between resistance genes of wheat and powdery mildew pathogens.

scholarly journals Introgression of the Powdery Mildew Resistance Genes Pm60 and Pm60b from Triticum urartu to Common Wheat Using Durum as a ‘Bridge’

Pathogens ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 25
Author(s):  
Qiang Zhang ◽  
Yinghui Li ◽  
Yiwen Li ◽  
Tzion Fahima ◽  
Qian-Hua Shen ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Common Wheat ◽  
Resistance Genes ◽  
Powdery Mildew Resistance ◽  
Resistance Breeding ◽  
Introgression Lines ◽  
Synthetic Hexaploid Wheat ◽  
Triticum Urartu ◽  
Mildew Resistance

Powdery mildew, caused by the fungus Blumeria graminis f. sp. tritici (Bgt), has limited wheat yields in many major wheat-production areas across the world. Introducing resistance genes from wild relatives into cultivated wheat can enrich the genetic resources for disease resistance breeding. The powdery mildew resistance gene Pm60 was first identified in diploid wild wheat Triticum urartu (T. urartu). In this study, we used durum as a ‘bridge’ approach to transfer Pm60 and Pm60b into hexaploid common wheat. Synthetic hexaploid wheat (SHW, AABBAuAu), developed by crossing T. urartu (AuAu) with durum (AABB), was used for crossing and backcrossing with common wheat. The Pm60 alleles were tracked by molecular markers and the resistance to powdery mildew. From BC1F1 backcross populations, eight recombinant types were identified based on five Pm60-flanking markers, which indicated different sizes of the introgressed chromosome segments from T. urartu. Moreover, we have selected two resistance-harboring introgression lines with high self-fertility, which could be easily used in wheat breeding system. Our results showed that the durum was an excellent ‘bridge’ for introducing the target gene from diploid T. urartu into the hexaploid cultivated wheat. Moreover, these introgression lines could be deployed in wheat resistance breeding programs, together with the assistance of the molecular markers for Pm60 alleles.

scholarly journals Fine mapping and identification of candidate genes for the peach powdery mildew resistance gene Vr3

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Neus Marimon ◽  
Jordi Luque ◽  
Pere Arús ◽  
Iban Eduardo
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Candidate Genes ◽  
Resistance Gene ◽  
Prunus Persica ◽  
Powdery Mildew Resistance ◽  
Disruptive Effect ◽  
Powdery Mildew Resistance Gene ◽  
Positional Candidate ◽  
Mildew Resistance

Abstract Powdery mildew is one of the major diseases of peach (Prunus persica), caused by the ascomycete Podosphaera pannosa. Currently, it is controlled through calendar-based fungicide treatments starting at petal fall, but an alternative is to develop resistant peach varieties. Previous studies mapped a resistance gene (Vr3) in interspecific populations between almond (‘Texas’) and peach (‘Earlygold’). To obtain molecular markers highly linked to Vr3 and to reduce the number of candidate genes, we fine-mapped Vr3 to a genomic region of 270 kb with 27 annotated genes. To find evidence supporting one of these positional candidate genes as being responsible of Vr3, we analyzed the polymorphisms of the resequences of both parents and used near-isogenic lines (NILs) for expression analysis of the positional candidate genes in symptomatic or asymptomatic leaves. Genes differentially expressed between resistant and susceptible individuals were annotated as a Disease Resistance Protein RGA2 (Prupe2G111700) or an Eceriferum 1 protein involved in epicuticular wax biosynthesis (Prupe2G112800). Only Prupe2G111700 contained a variant predicted to have a disruptive effect on the encoded protein, and was overexpressed in both heterozygous and homozygous individuals containing the Vr3 almond allele, compared with susceptible individuals. This information was also useful to identify and validate molecular markers tightly linked and flanking Vr3. In addition, the NILs used in this work will facilitate the introgression of this gene into peach elite materials, alone or pyramided with other known resistance genes such as peach powdery mildew resistance gene Vr2.

Identification and SSR Mapping of Two Powdery Mildew Resistance Genes in Wild Emmer (Triticum dicoccoides) Accessions IW3 and IW10

2009 ◽  
Vol 35 (5) ◽  
pp. 761-767 ◽  
Author(s):  
Gen-Qiao LI ◽  
Ti-Lin FANG ◽  
Hong-Tao ZHANG ◽  
Chao-Jie XIE ◽  
Zuo-Min YANG ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Genes ◽  
Powdery Mildew Resistance ◽  
Triticum Dicoccoides ◽  
Wild Emmer ◽  
Mildew Resistance
Sign in / Sign up

Export Citation Format

Share Document