Transposable elements as plant transformation vectors for long stretches of foreign DNA

1995 ◽  
Vol 91-91 (6-7) ◽  
pp. 899-906 ◽  
Author(s):  
E. G. Lebel ◽  
J. Masson ◽  
A. Bogucki ◽  
J. Paszkowski
Keyword(s):  
Transposable Elements ◽  
Plant Transformation ◽  
Foreign Dna ◽  
Transformation Vectors

scholarly journals Zinc Finger Nuclease and Homing Endonuclease-Mediated Assembly of Multigene Plant Transformation Vectors

2011 ◽  
Vol 158 (1) ◽  
pp. 132-144 ◽  
Author(s):  
Vardit Zeevi ◽  
Zhuobin Liang ◽  
Uri Arieli ◽  
Tzvi Tzfira
Keyword(s):  
Zinc Finger ◽  
Plant Transformation ◽  
Homing Endonuclease ◽  
Zinc Finger Nuclease ◽  
Transformation Vectors

Unexpected Stability of mariner Transgenes in Drosophila

Genetics ◽  
2002 ◽  
Vol 160 (2) ◽  
pp. 527-535 ◽  
Author(s):  
Elena R Lozovsky ◽  
Dmitry Nurminsky ◽  
Ernst A Wimmer ◽  
Daniel L Hartl
Keyword(s):  
Drosophila Melanogaster ◽  
Transposable Elements ◽  
Baseline Level ◽  
Inverted Repeats ◽  
Exogenous Dna ◽  
Eye Color ◽  
Transformation Vectors

Abstract A number of mariner transformation vectors based on the mauritiana subfamily of transposable elements were introduced into the genome of Drosophila melanogaster and examined for their ability to be mobilized by the mariner transposase. Simple insertion vectors were constructed from single mariner elements into which exogenous DNA ranging in size from 1.3 to 4.5 kb had been inserted; composite vectors were constructed with partial or complete duplications of mariner flanking the exogenous DNA. All of the simple insertion vectors showed levels of somatic and germline excision that were at least 100-fold lower than the baseline level of uninterrupted mariner elements. Although composite vectors with inverted duplications were unable to be mobilized at detectable frequencies, vectors with large direct duplications of mariner could be mobilized. A vector consisting of two virtually complete elements flanking exogenous DNA yielded a frequency of somatic eye-color mosaicism of ~10% and a frequency of germline excision of 0.04%. These values are far smaller than those observed for uninterrupted elements. The results imply that efficient mobilization of mariner in vivo requires the presence and proper spacing of sequences internal to the element as well as the inverted repeats.

Modified binary plant transformation vectors with the wild-type gene encoding NPTII

Gene ◽  
1992 ◽  
Vol 122 (2) ◽  
pp. 383-384 ◽  
Author(s):  
Raju S.S. Datia ◽  
Joe K. Hammerlindl ◽  
Barry Panchuk ◽  
Lawrence E. Pelcher ◽  
Wilf Keller
Keyword(s):  
Plant Transformation ◽  
Wild Type ◽  
Gene Encoding ◽  
Type Gene ◽  
Transformation Vectors

Modified binary plant transformation vectors with the wild-type gene encoding NPTII

Gene ◽  
1993 ◽  
Vol 129 (2) ◽  
pp. 311
Author(s):  
Raju S.S. Datia ◽  
Joe K. Hammeriindl ◽  
Barry Panchuk ◽  
Lawrence E. Pelcher ◽  
Wilf Keller
Keyword(s):  
Plant Transformation ◽  
Wild Type ◽  
Gene Encoding ◽  
Type Gene ◽  
Transformation Vectors

Yeast-based recombineering of DNA fragments into plant transformation vectors by one-step transformation

2007 ◽  
Vol 26 (12) ◽  
pp. 2111-2117 ◽  
Author(s):  
Yukio Nagano ◽  
Syoko Takao ◽  
Takahiro Kudo ◽  
Ei’ichi Iizasa ◽  
Toyoaki Anai
Keyword(s):  
Plant Transformation ◽  
Dna Fragments ◽  
One Step ◽  
Transformation Vectors
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