scholarly journals DNA fingerprinting detects genetic variability in the pearl millet downy mildew pathogen (Sclerospora graminicola)

1995 ◽  
Vol 91-91 (6-7) ◽  
pp. 856-861 ◽  
Author(s):  
J. G. Sastry ◽  
W. Ramakrishna ◽  
S. Sivaramakrishnan ◽  
R. P. Thakur ◽  
V. S. Gupta ◽  
...  
2003 ◽  
Vol 41 (11/12) ◽  
pp. 361-374 ◽  
Author(s):  
R. Singru ◽  
S. Sivaramakrishnan ◽  
R. P. Thakur ◽  
V. S. Gupta ◽  
P. K. Ranjekar

2017 ◽  
Vol 16 ◽  
pp. 18-20 ◽  
Author(s):  
S. Chandra Nayaka ◽  
H. Shekar Shetty ◽  
C.Tara Satyavathi ◽  
Rattan S. Yadav ◽  
P.B.Kavi Kishor ◽  
...  

2021 ◽  
Vol 29 (2) ◽  
pp. 110-113
Author(s):  
Sapna Jaiswal ◽  
Rajni S. Sasode ◽  
R.K. Pandya ◽  
Pramod Kumar Gupta

2005 ◽  
Vol 32 (3) ◽  
pp. 267 ◽  
Author(s):  
N. P. Geetha ◽  
K. N. Amruthesh ◽  
R. G. Sharathchandra ◽  
H. S. Shetty

Phenylalanine ammonia lyase (PAL) activity was studied in pearl millet cultivars with different levels of resistance to the downy mildew disease caused by Sclerospora graminicola, an important oomycete pathogen. PAL activity was elevated in resistant host cultivar and decreased in susceptible cultivars following downy mildew pathogen infection. The enzyme activation varied between cultivars and was correlated with the degree of resistance to downy mildew disease. The induction of PAL as a response to pathogen inoculation was further corroborated by a time-course study in seedlings and cultured cells of pearl millet. The level of PAL activity was highest at 1.5 h in cultured cells and 4 h in seedlings of resistant host cultivar after inoculation with Sclerospora graminicola. Further studies on PAL activity in different tissues of seedlings showed highest enzyme activity in the young growing region of the root of the resistant host cultivars. The accumulation of wall-bound phenolics and lignin was higher in the resistant cultivar seedlings as evidenced by phloroglucinol–HCl staining and p-coumaric acid assay. The temporal changes in lignin concentration and the concentration of soluble phenolics were greater in root tissues of resistant cultivars than in those of susceptible cultivars. Treatment of resistant seedlings with a PAL inhibitor, α-aminooxy-β-phenylpropionic acid, resulted in the enhancement of the enzyme activity, whereas in the presence of 1 mm trans-cinnamic acid the pathogen-induced PAL was completely inhibited. Treatment of pearl millet seedlings with exogenously applied PAL inhibitors induced downy mildew disease susceptibility in the resistant pearl millet cultivar, consistent with direct involvement of PAL in downy mildew resistance. Results are discussed with respect to the presumed importance of host phenolic compounds and lignin accumulation and its relation to PAL activation as a response to the pathogen infection.


Plant Disease ◽  
1998 ◽  
Vol 82 (7) ◽  
pp. 791-793 ◽  
Author(s):  
S. D. Singh ◽  
B. S. Talukdar

Inheritance of downy mildew (Sclerospora graminicola) resistance in pearl millet (Pennisetum glaucum) was studied in a cross involving DMRP (downy mildew resistant Pennisetum) 292 and DMSP (downy mildew susceptible Pennisetum) 23. DMRP 292, derived from IP 18292, and DMSP 23, derived from Tift 23DB, were made homozygous for downy mildew resistance-susceptibility by three generations of single plant selection followinginoculations with homogenized sporangial population of S. graminicola from the ICRISAT Asia Center. Potted seedlings of parental lines, F1, F2, and BC progenies were evaluated for their reactions to downy mildew following inoculation. The results show that complete resistance to downy mildew in DMRP 292 is controlled by a single dominant gene. Because of the simple mode of inheritance, resistance utilization from DMRP 292 is simple and straightforward. We designate this gene as Rsg1.


2011 ◽  
Vol 34 (2) ◽  
pp. 779-791 ◽  
Author(s):  
Neerakkal Sujeeth ◽  
Ramachandra K. Kini ◽  
Sekhar Shailasree ◽  
Eelco Wallaart ◽  
Shekar H. Shetty ◽  
...  

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