Study of microspore-culture responsiveness in oilseed rape (Brassica napus L.) by comparative mapping of a F2 population and two microspore-derived populations

1995 ◽  
Vol 91-91 (6-7) ◽  
pp. 841-847 ◽  
Author(s):  
S. Cloutier ◽  
M. Cappadocia ◽  
B. S. Landry
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Comparative Mapping ◽  
Microspore Culture ◽  
Brassica Napus L ◽  
F2 Population

scholarly journals Utilisation of doubled haploids in winter oilseed rape (Brassica napus L.) breeding

2012 ◽  
Vol 38 (No. 1) ◽  
pp. 50-54 ◽  
Author(s):  
V. Kučera ◽  
M. Vyvadilová ◽  
M. Klíma
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Doubled Haploids ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
The Czech Republic ◽  
Yield Parameters ◽  
The World

A survey of development and prospects of the utilisation of doubled haploid techniques in rapeseed breeding in the world and in the Czech Republic is presented. The first utilisation of spontaneously occurred haploids from Brassica napus inbreeding programmes is described. The development of techniques of anther and later microspore culture is outlined. Special emphasis is given to the practical use of doubled haploids for the improvement of the effectiveness of breeding new productive cultivars. Some partial results of evaluation of yield parameters and resistance to important diseases in the obtained doubled haploid lines of winter oilseed rape are shown. The literary review and present results indicate, that the doubled haploid technique can be effectively used for the development of homozygous oilseed rape lines as an alternative to conventional methods.

Homoeologous loci control the accumulation of seed glucosinolates in oilseed rape (Brassica napus)

Genome ◽  
2003 ◽  
Vol 46 (3) ◽  
pp. 454-460 ◽  
Author(s):  
P M Howell ◽  
A G Sharpe ◽  
D J Lydiate
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Phenotypic Variation ◽  
Comparative Mapping ◽  
Genetic Maps ◽  
Glucosinolate Content ◽  
Marker Assisted Breeding ◽  
Brassica Napus L ◽  
Backcross Populations ◽  
Seed Glucosinolates

The genetic control of seed glucosinolate content in oilseed rape was investigated using two intervarietal backcross populations. Four QTLs segregating in the population derived from a Brassica napus L.'Victor' × Brassica napus L.'Tapidor' cross, together accounting for 76% of the phenotypic variation, were mapped. Three of these loci also appeared to control the accumulation of seed glucosinolates in a Brassica napus L. 'Bienvenu' × 'Tapidor' cross, and accounted for 86% of the phenotypic variation. The three QTLs common to both populations mapped to homoeologous regions of the B. napus genome, suggesting that seed glucosinolate accumulation is controlled by duplicate genes. It was possible to extend the comparative analysis of QTLs controlling seed glucosinolate accumulation by aligning the published genetic maps generated by several research groups. This comparative mapping demonstrated that high-glucosinolate varieties often carry low-glucosinolate alleles at one or more of the loci controlling seed glucosinolate accumulation.Key words: backcross, comparative mapping, genetic linkage map, marker-assisted breeding, QTL.

In vitro selection of herbicide resistance in microspore-derived embryos of oilseed rape (Brassica napus L.)

2006 ◽  
Vol 3 (1) ◽  
pp. 33-38
Author(s):  
Xu Ling ◽  
Zhang Guo-Qing ◽  
Gu Hong-Hui ◽  
He Yun ◽  
Zhou Wei-Jun
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
In Vitro Selection ◽  
Chromosome Doubling ◽  
Microspore Culture ◽  
Brassica Napus L ◽  
Regenerated Plants ◽  
Short Time ◽  
Selection Of

AbstractUsing four oilseed rape (Brassica napus L.) F1 hybrids (7039, 7040, 282 and 5102) as donor plants for microspore culture, the experiment was conducted to select glyphosate- and haloxyfop-resistant embryos through application of these substances to the cultural media with microspore-derived embryos in vitro. Genotypes 7039 and 7040 were used to select glyphosate-resistant regenerated plants, and genotypes 282 and 5102 to select haloxyfop-resistant plants. The embryos at cotyledonary stage were grown on glyphosate- and haloxyfop-containing MS-2 medium for 2 weeks. The non-resistant embryos collapsed after a short time, while the resistant ones turned green and survived for 2 weeks. Transferred into the normal MS-2 medium for further plant regeneration, the regenerated plants from green embryos showed tolerance to 0.25% sprayed glyphosate, indicating the effectiveness and reliability of this in vitro selection method. When the regenerated plants selected from 0.02% haloxyfop were sprayed with 0.05% haloxyfop, most of them grew well; however, the survival rate of the regenerated plants from 0.01% haloxyfop-containing medium was lower. The present experiment indicates that the use of 0.02% haloxyfop in the selection of haloxyfop-resistant plants was more promising than that of 0.01%. The chromosome doubling efficiency of regenerated plants reached 34% and 52% after being treated with 170 mg/l colchicine for 20 and 30 h, respectively.

scholarly journals Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape (Brassica napus L.) against Fungal Pathogens

2020 ◽  
Vol 21 (22) ◽  
pp. 8740
Author(s):  
Daria Chlebek ◽  
Artur Pinski ◽  
Joanna Żur ◽  
Justyna Michalska ◽  
Katarzyna Hupert-Kocurek
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Oilseed Rape ◽  
Plant Growth Promotion ◽  
Fungal Pathogens ◽  
Growth Promotion ◽  
Genome Mining ◽  
Plant Colonization ◽  
Brassica Napus L

Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of Pseudomonas fluorescens BRZ63, a new endophyte of oilseed rape (Brassica napus L.) against Rhizoctonia solani W70, Colletotrichum dematium K, Sclerotinia sclerotiorum K2291, and Fusarium avenaceum. In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of Arabidopsis thaliana Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion.

Amino acid contents and transport in oilseed rape (Brassica napus L.) under different nitrogen conditions

Planta ◽  
2004 ◽  
Vol 221 (3) ◽  
pp. 328-338 ◽  
Author(s):  
Jens Tilsner ◽  
Nina Kassner ◽  
Christine Struck ◽  
Gertrud Lohaus
Keyword(s):  
Amino Acid ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Brassica Napus L ◽  
Amino Acid Contents ◽  
Nitrogen Conditions

Effects of abscisic acid and temperature on erucic acid accumulation in oilseed rape (Brassica napus L.)

1997 ◽  
Vol 150 (4) ◽  
pp. 414-419 ◽  
Author(s):  
Jeroen A. Wilmer ◽  
Johannes P.F.G. Helsper ◽  
Linus H.W. van der Plas
Keyword(s):  
Abscisic Acid ◽  
Brassica Napus ◽  
Erucic Acid ◽  
Oilseed Rape ◽  
Brassica Napus L ◽  
Acid Accumulation
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