Genotype, plant, bud size and media factors affecting anther culture of cauliflowers (Brassica oleracea var. botrytis)

1990 ◽  
Vol 79 (1) ◽  
pp. 33-38 ◽  
Author(s):  
C. Phippen ◽  
D. J. Ockendon
Keyword(s):  
Brassica Oleracea ◽  
Anther Culture ◽  
Factors Affecting ◽  
Bud Size

scholarly journals Association between floral bud size and developmental stage in soybean microspores

2003 ◽  
Vol 46 (4) ◽  
pp. 515-520 ◽  
Author(s):  
Mozart da Silva Lauxen ◽  
Eliane Kaltchuk- Santos ◽  
Ching -yeh Hu ◽  
Sidia Maria Callegari- Jacques ◽  
Maria Helena Bodanese-Zanettini
Keyword(s):  
Developmental Stage ◽  
Anther Culture ◽  
Developmental Stages ◽  
Size Group ◽  
Soybean Cultivars ◽  
Advanced Stages ◽  
Bud Size ◽  
Microspore Stage ◽  
Floral Bud

This study was carried out to establish the association between floral bud size and the corresponding microspore developmental stages for Brazilian soybean cultivars. Microspore developmental stage distributions were examined in young buds from cv Década, IAS5 and RS7. The data indicated that for a given bud-size group, the microspores of different cultivars were at different developmental stages, with cv RS7 and Década distributed at the youngest and cv IAS5 at the most advanced stages. Microspore stages distribution were also compared among the ten anthers of the same bud of the above cultivars. The ten anthers from a given bud were clearly distributed at different developmental stages. Caution should be exercised when adopting the standard anther culture practice of using the microspore stage of one anther to represent the entire bud.

EMBRYOGENESIS AND HAPLOID PLANT PRODUCTION FROM ANTHER CULTURE OF CABBAGE (Brassica oleracea var. capitata L.)

1985 ◽  
Vol 65 (4) ◽  
pp. 1033-1037 ◽  
Author(s):  
M. S. CHIANG ◽  
S. FRECHETTE ◽  
C. G. KUO ◽  
CALVIN CHONG ◽  
S. J. DELAFIELD
Keyword(s):  
Tissue Culture ◽  
Brassica Oleracea ◽  
Anther Culture ◽  
Hydrolysis Product ◽  
Breeding Population ◽  
Plant Production ◽  
Haploid Plant ◽  
Single Plant ◽  
Embryo Yield

Thirteen out of nineteen single plant cabbage (Brassica oleracea) selections from a clubroot-resistant breeding population with known variation in glucosinolate composition yielded embryos from cultured anthers. The highest embryo yield was obtained with a line that contained the lowest content of total glucosinolates and which was free of the glucosinolate hydrolysis product, goitrin. Of 191 regenerants examined from five selections, 23 haploids, 105 diploids, 162 tetraploids, and 1 aneuploid were identified.Key words: Tissue culture, glucosinolates, clubroot, cabbage, embryo, haploid

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