Characterization of a sunflower (Helianthus annuus L.) mutant, deficient in carotenoid synthesis and abscisic-acid content, induced by in-vitro tissue culture

1993 ◽  
Vol 87 (1-2) ◽  
pp. 65-69 ◽  
Author(s):  
M. Fambrini ◽  
C. Pugliesi ◽  
P. Vernieri ◽  
G. Giuliano ◽  
S. Baroncelli
Keyword(s):  
Tissue Culture ◽  
Abscisic Acid ◽  
Helianthus Annuus ◽  
Acid Content ◽  
In Vitro Tissue Culture ◽  
Helianthus Annuus L ◽  
Carotenoid Synthesis ◽  
Abscisic Acid Content

scholarly journals Characterization of maize lines differing in leaf abscisic acid content in the field. 1 abscisic acid physiology

1995 ◽  
Vol 75 (1) ◽  
pp. 67-73 ◽  
Author(s):  
S PEKIC ◽  
R STIKIC ◽  
L TOMLJANOVIC ◽  
V ANDJELKOVIC ◽  
M IVANOVIC ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Acid Content ◽  
Abscisic Acid Content

scholarly journals Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture

2021 ◽  
Vol 28 (1) ◽  
Author(s):  
Renata Orłowska
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Taguchi Method ◽  
Culture Conditions ◽  
Dna Demethylation ◽  
Silver Ion ◽  
Donor Plant ◽  
In Vitro Tissue Culture ◽  
Induced Variation

Abstract Background Somatic embryogenesis is a phenomenon carried out in an environment that generates abiotic stress. Thus, regenerants may differ from the source of explants at the morphological, genetic, and epigenetic levels. The DNA changes may be the outcome of induction media ingredients (i.e., copper and silver ions) and their concentrations and time of in vitro cultures. Results This study optimised the level of copper and silver ion concentration in culture media parallel with the induction medium longevity step towards obtaining barley regenerants via somatic embryogenesis with a minimum or maximum level of tissue culture-induced differences between the donor plant and its regenerants. The optimisation process is based on tissue culture-induced variation evaluated via the metAFLP approach for regenerants derived under varying in vitro tissue culture conditions and exploited by the Taguchi method. In the optimisation and verification experiments, various copper and silver ion concentrations and the different number of days differentiated the tested trials concerning the tissue culture-induced variation level, DNA demethylation, and de novo methylation, including symmetric (CG, CHG) and asymmetric (CHH) DNA sequence contexts. Verification of optimised conditions towards obtaining regenerants with minimum and maximum variability compared to donor plants proved useful. The main changes that discriminate optimised conditions belonged to DNA demethylation events with particular stress on CHG context. Conclusions The combination of tissue culture-induced variation evaluated for eight experimental trials and implementation of the Taguchi method allowed the optimisation of the in vitro tissue culture conditions towards the minimum and maximum differences between a source of tissue explants (donor plant) and its regenerants from somatic embryos. The tissue culture-induced variation characteristic is mostly affected by demethylation with preferences towards CHG sequence context.

In vitro tissue culture in breeding programs of leguminous pulses: use and current status

2016 ◽  
Vol 127 (3) ◽  
pp. 543-559 ◽  
Author(s):  
Ileana Gatti ◽  
Fernanda Guindón ◽  
Carolina Bermejo ◽  
Andrea Espósito ◽  
Enrique Cointry
Keyword(s):  
Tissue Culture ◽  
Current Status ◽  
Breeding Programs ◽  
In Vitro Tissue Culture
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