Somatic hybrids between Solanum etuberosum and diploid, tuber bearing Solanum clones

1994 ◽  
Vol 89 (6) ◽  
pp. 775-782 ◽  
Author(s):  
R. G. Novy ◽  
J. P. Helgeson
Keyword(s):  
Somatic Hybrids ◽  
Solanum Etuberosum

Cytological characterization of potato - Solanum etuberosum somatic hybrids and their backcross progenies by genomic in situ hybridization

Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 987-992 ◽  
Author(s):  
F Dong ◽  
R G Novy ◽  
J P Helgeson ◽  
J Jiang
Keyword(s):  
In Situ Hybridization ◽  
Dna Marker ◽  
Somatic Hybrids ◽  
Molecular Cytogenetics ◽  
Genomic In Situ Hybridization ◽  
Preferential Pairing ◽  
Potato Germplasm ◽  
Solanum Etuberosum

Four somatic hybrids derived from a diploid wild species Solanum etuberosum and a diploid tuber-bearing Solanum clone 463-4, together with five BC1 and three BC2 plants, were analyzed by genomic in situ hybridization (GISH). None of the four somatic hybrids had the expected chromosome constitutions, i.e., 24 chromosomes from each fusion parent. Either one chromosome from S. etuberosum or one from the potato parent 463-4 was lost in the hybrids. Three BC1 plants had exactly one set of S. etuberosum chromosomes. The other two BC1 plants either had one extra or one fewer S. etuberosum chromosome, possibly because their somatic hybrid parents had an extra or had lost one S. etuberosum chromosome. The presence of one set, or close to one set, of S. etuberosum chromosomes in all BC1 plants suggests a preferential pairing and segregation of the S. etuberosum chromosomes in the somatic hybrids. Two of the three BC2 plants had 52 chromosomes, deviating significantly from the expected chromosome number of 48. These results suggest poor pairing between S. etuberosum and S. tuberosum chromosomes in the BC1 plants. The present study demonstrates the importance of combining GISH and DNA marker analysis for a thorough characterization of potato germplasm containing chromosomes from different species.Key words: potato germplasm, Solanum etuberosum, molecular cytogenetics.

Analysis of plastome and chondriome genome types in potato somatic hybrids from Solanum tuberosum × Solanum etuberosum

Genome ◽  
2014 ◽  
Vol 57 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Jagesh K. Tiwari ◽  
Poonam Chandel ◽  
Bir Pal Singh ◽  
Vinay Bhardwaj
Keyword(s):  
Solanum Tuberosum ◽  
Somatic Hybrids ◽  
Gene Pools ◽  
Organelle Genomes ◽  
Cpdna Markers ◽  
Genomic Regions ◽  
Solanum Etuberosum ◽  
Unique Potential ◽  
Conserved Genomic Regions ◽  
Mtdna Markers

Cytoplasm types of the potato somatic hybrids from Solanum tuberosum × Solanum etuberosum were analysed using chloroplast (cp) and mitochondrial (mt) organelle genomes-specific markers. Of the 29 markers (15 cpDNA and 14 mtDNA) amplified in the 26 genotypes, 5 cpDNA (H3, NTCP4, NTCP8, NTCP9, and ALC1/ALC3) and 13 mtDNA markers showed polymorphism. The cluster analysis based on the mtDNA markers detected higher diversity compared with the cpDNA markers. Presence of new mtDNA fragments of the markers, namely, T11-2, Nsm1, pumD, Nsm3, and Nsm4, were observed, while monomorphic loci revealed highly conserved genomic regions in the somatic hybrids. The study revealed that the somatic hybrids had diverse cytoplasm types consisting predominantly of T-, W-, and C-, with a few A- and S-type cp genomes; and α-, β-, and γ-type mt genomes. Somatic hybridization has unique potential to widen the cytoplasm types of the cultivated gene pools from wild species through introgression by breeding methods.

Genetical Studies on Progenies of Somatic Hybrids between Brassica napus and Descurainia Sophia

2009 ◽  
Vol 35 (6) ◽  
pp. 1044-1050
Author(s):  
Ru-Ying XIN ◽  
Rong-Zhan GUAN ◽  
Li-Jun ZHANG ◽  
Shu-Hui JIANG ◽  
Hong-Sheng ZHANG ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Somatic Hybrids ◽  
Descurainia Sophia

scholarly journals Genetic composition of interspecific potato somatic hybrids and autofused 4x plants evaluated by DArT and cytoplasmic DNA markers

2016 ◽  
Vol 35 (6) ◽  
pp. 1345-1358 ◽  
Author(s):  
Paulina Smyda-Dajmund ◽  
Jadwiga Śliwka ◽  
Iwona Wasilewicz-Flis ◽  
Henryka Jakuczun ◽  
Ewa Zimnoch-Guzowska
Keyword(s):  
Dna Markers ◽  
Somatic Hybrids ◽  
Genetic Composition ◽  
Cytoplasmic Dna

Biased chloroplast and mitochondrial transmission in somatic hybrids of Allium ampeloprasum L. and Allium cepa L.

Plant Science ◽  
1998 ◽  
Vol 131 (2) ◽  
pp. 219-228 ◽  
Author(s):  
J Buiteveld ◽  
W Kassies ◽  
R Geels ◽  
M.M van Lookeren Campagne ◽  
E Jacobsen ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Somatic Hybrids ◽  
Allium Ampeloprasum ◽  
Allium Cepa L ◽  
Mitochondrial Transmission

scholarly journals Drought and Saline Stress Tolerance Induced in Somatic Hybrids of Solanum chacoense and Potato Cultivars by Using Mismatch Repair Deficiency

Agriculture ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 696
Author(s):  
Imola Molnár ◽  
Lavinia Cozma ◽  
Tünde-Éva Dénes ◽  
Imre Vass ◽  
István-Zoltán Vass ◽  
...  
Keyword(s):  
Climate Change ◽  
Mismatch Repair ◽  
Crop Production ◽  
Somatic Hybrids ◽  
Potato Cultivars ◽  
Solanum Chacoense ◽  
Saline Stress ◽  
Severe Drought ◽  
Mismatch Repair Deficiency ◽  
Repair Deficiency

Global climate change, especially when involving drought and salinity, poses a major challenge to sustainable crop production, causing severe yield losses. The environmental conditions are expected to further aggravate crop production in the future as a result of continuous greenhouse gas emissions, causing further temperature rise and leading to increased evapotranspiration, severe drought, soil salinity, as well as insect and disease threats. These suboptimal growth conditions have negative impact on plant growth, survival, and crop yield. Potato is well known as a crop extremely susceptible to drought, which is primarily attributed to its shallow root system. With potato being the fourth major food crop, increasing potato productivity is thus important for food security and for feeding global population. To maintain a sustainable potato production, it is necessary to develop stress tolerant potato cultivars that cope with the already ongoing climate change. The aim of our study is to analyze the response of potato somatic hybrids to drought and salt stress under in vitro conditions; the somatic hybrids studied are the wild relative Solanum chacoense (+) Solanum tuberosum, with or without mismatch repair deficiency (MMR). Upon this selection of drought and salt tolerant genotypes, somatic hybrids and their parents were phenotyped on a semi-automated platform, and lines tolerant to medium water scarcity (20% compared to 60% soil water capacity) were identified. Although none of the parental species were tolerant to drought, some of the MMR-deficient somatic hybrids showed tolerance to drought and salt as a new trait.

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