Production of intertribal somatic hybrids between Brassica napus L. and Lesquerella fendleri (Gray) Wats

1996 ◽  
Vol 93 (8) ◽  
pp. 1242-1250 ◽  
Author(s):  
M. Skarzhinskaya ◽  
M. Landgren ◽  
K. Glimelius
Keyword(s):  
Brassica Napus ◽  
Somatic Hybrids ◽  
Brassica Napus L ◽  
Lesquerella Fendleri

scholarly journals Comparing the Resynthesis of Brassica napus L. by Interspecific Somatic and Sexual Hybridization. I. Producing and Identifying Hybrids

1994 ◽  
Vol 119 (4) ◽  
pp. 808-815 ◽  
Author(s):  
Richard H. Ozminkowski ◽  
Pablo Jourdan
Keyword(s):  
Polyethylene Glycol ◽  
Brassica Napus ◽  
Parental Species ◽  
Somatic Hybrids ◽  
Embryo Rescue ◽  
Leaf Tissue ◽  
Brassica Napus L ◽  
Intraspecific Hybridization ◽  
Sexual Hybridization ◽  
Total Dna

Brassica napus (genome aacc), a natural allotetraploid derived from hybridization between B. oleracea L. (genome cc) and B. rapa L. (genome aa), was synthesized by sexual and somatic interspecific hybridizations from the same parent plants to compare the two methods of combining genomes and assess the genetic consequences of bypassing the gametophytic phase before hybrid formation. Highly heterozygous species parents were first produced by intraspecific hybridization between two subspecies each of B. oleracea and B. rapa. Leaf tissue from young plants of both parental species served as a source of protoplasts for fusion; the same plants were later used for crosses. Seventy-two somatic hybrids were produced using a polyethylene glycol-mediated fusion protocol and 27 sexual hybrids were obtained by embryo rescue. Somatic hybrids were produced between one B. oleracea and two sibling B. rapa plants. Sexual hybrids were successfully produced with only one of the two B. rapa siblings. Hybrids were identified by morphology, isozyme patterns, and total DNA content. Although fertile allotetraploid somatic hybrids were obtained within 7 months after seeding parent lines, >1 year was required to produce fertile sexual hybrids.

Very long chain and hydroxylated fatty acids in offspring of somatic hybrids between Brassica napus and Lesquerella fendleri

1999 ◽  
Vol 99 (1-2) ◽  
pp. 108-114 ◽  
Author(s):  
M. Schröder-Pontoppidan ◽  
M. Skarzhinskaya ◽  
C. Dixelius ◽  
S. Stymne ◽  
K. Glimelius
Keyword(s):  
Fatty Acids ◽  
Brassica Napus ◽  
Somatic Hybrids ◽  
Lesquerella Fendleri ◽  
Hydroxylated Fatty Acids ◽  
Long Chain

Genome organization of Brassica napus and Lesquerella fendleri and analysis of their somatic hybrids using genomic in situ hybridization

Genome ◽  
1998 ◽  
Vol 41 (5) ◽  
pp. 691-701 ◽  
Author(s):  
Marina Skarzhinskaya ◽  
Jan Fahleson ◽  
Kristina Glimelius ◽  
Armand Mouras
Keyword(s):  
In Situ Hybridization ◽  
Brassica Napus ◽  
Somatic Hybrids ◽  
Chromosome Translocation ◽  
Genomic In Situ Hybridization ◽  
Lesquerella Fendleri ◽  
Hybrid Plants ◽  
Transformed Root Cultures ◽  
Asymmetric Fusion

Construction of Brassica napus (2n = 38) and Lesquerella fendleri (2n = 12) karyotypes revealed that B. napus contains 10 pairs of metacentric, 5 pairs of submetacentric, and 4 pairs of subtelocentric chromosomes, while L. fendleri contains 6 pairs of metacentric chromosomes that are, on average, 1.5 times longer than those of B. napus. By analysing the karyotypes and performing genomic in situ hybridization (GISH), the chromosome complements of somatic hybrids produced between Brassica napus (+) Lesquerella fendleri were studied. Protoplasts fused with no pretreatment, symmetric fusions, resulted in hybrids containing L. fendleri chromosomes in numbers varying from 2 chromosomes to 2 chromosome complements. In the asymmetric fusion experiments, in which L. fendleri protoplasts were irradiated before fusion, plants with from 38 to more than 76 chromosomes were obtained. In the hybrids with 38 chromosomes, the presence of L. fendleri chromosomes was not detected by GISH analysis, even though L. fendleri DNA was revealed by Southern blotting. Intra- and inter-genomic recombinations were observed in hybrids from both symmetric and asymmetric fusions. The rearrangements were more prevalent in plants resulting from asymmetric fusions. Modifications of the phenotype and reduced fertility of the hybrid plants were correlated with increased dosages of the L. fendleri genome. At least one genome complement of L. fendleri was required to express its morphological features.Key words: Agrobacterium rhizogenes transformed root cultures, Brassica napus, Lesquerella fendleri, somatic hybrids, chromosome translocation.

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