Isolation of mitochondrial DNA sequences that distinguish male-sterility-inducing cytoplasms in Sorghum bicolor (L.) Moench

1995 ◽  
Vol 90 (7-8) ◽  
pp. 1180-1187 ◽  
Author(s):  
G.-W. Xu ◽  
Y.-X. Cui ◽  
K. F. Schertz ◽  
G. E. Hart
Genome ◽  
2000 ◽  
Vol 43 (6) ◽  
pp. 988-1002 ◽  
Author(s):  
Dinakar Bhattramakki ◽  
Jianmin Dong ◽  
Ashok K Chhabra ◽  
Gary E Hart

We report the development, testing, and use (for genetic mapping) of a large number of polymerase chain reaction (PCR) primer sets that amplify DNA simple sequence repeat (SSR) loci of Sorghum bicolor (L.) Moench. Most of the primer sets were developed from clones isolated from two sorghum bacterial artificial chromosome (BAC) libraries and three enriched sorghum genomic-DNA (gDNA) libraries. A few were developed from sorghum DNA sequences present in public databases. The libraries were probed with radiolabeled di- and trinucleotide oligomers, the BAC libraries with four and six oligomers, respectively, and the enriched gDNA libraries with four and three oligomers, respectively. Both types of libraries were markedly enriched for SSRs relative to a size-fractionated gDNA library studied earlier. However, only 2% of the sequenced clones obtained from the size-fractionated gDNA library lacked a SSR, whereas 13% and 17% of the sequenced clones obtained from the BAC and enriched gDNA libraries, respectively, lacked a SSR. Primer sets were produced for 313 SSR loci. Two-hundred sixty-six (85%) of the loci were amplified and 165 (53%) of the loci were found to be polymorphic in a population composed of 18 diverse sorghum lines. (AG/TC)n and (AC/TG)n repeats comprised 91% of the dinucleotide SSRs and 52% of all of the SSRs at the polymorphic loci, whereas four types of repeats comprised 66% of the trinucleotide SSRs at the loci. Primer sequences are reported for the 165 polymorphic loci and for eight monomorphic loci that have a high degree of homology to genes. Also reported are the genetic map locations of 113 novel SSR loci (including four SSR-containing gene loci) and a linkage map composed of 147 SSR loci and 323 RFLP (restriction fragment length polymorphism) loci. The number of SSR loci per linkage group ranges from 8 to 30. The SSR loci are distributed relatively evenly throughout approximately 75% of the 1406-cM linkage map, but segments of five linkage groups comprising about 25% of the map either lack or contain few SSR loci. Mapping of SSR loci isolated from BAC clones located to these segments is likely to be the most efficient method for placing SSR loci in the segments.Key words: DNA libraries, linkage mapping, Sorghum bicolor, SSRs.


Euphytica ◽  
2006 ◽  
Vol 154 (1-2) ◽  
pp. 153-164 ◽  
Author(s):  
Belum V. S. Reddy ◽  
S. Ramesh ◽  
P. Sanjana Reddy ◽  
B. Ramaiah

2021 ◽  
Author(s):  
Lokesh Kumar Verma Kumar Verma ◽  
B. D. Biradar ◽  
S. S. Patil ◽  
S. N. Chattannavar ◽  
V. S. Kubsad

Abstract In sorghum, milo source of male sterility has been used for developing all the hybrids so far and it created the narrow cytoplasmic base. In addition, it also narrows down the nuclear diversity which may be the invitation for the outbreak of pests and diseases in the near future. To handle this hazard new diverse sources like A2, A3, A4, maldandi, VZM and G1 have been discovered. The availability of restorers on these sources is very difficult hence their utilization is also a challenging task. The present study was, therefore, carried out to test different sorghum lines for restoration behavior on two milo lines (104A and 401A) and one maldandi (M31-2A) with more emphasis on maldnadi source because of its added advantage of enhancing grain size and pest tolerance. In rabi-2017, 125 genotypes were tested for the restoration behavior on these sources, and five restorers viz., IS 19389, IS 995, IS 28389, IS 26046 (BRJ 67 − 8) and DSMR-8 restored fertility on 104A, two lines viz., IS 29335 and IS 26046 restored on 401A, while on M31-2A six lines viz., IS 19975, BRJ-67-4 (DSMR-4), BRJ 67 − 8 (DSMR-8), BRJ 67 − 16, BRJ 67 − 19, BRJ 67 − 21 restored the fertility. only two lines DSMR-8 and DSMR-4 exhibited stable, consistent and strong restoration (> 90% seed set) across the seasons (rabi-2017 and kharif-2017) and years (2017 and rabi-2018). All the used MS lines exhibited the restoration complexity in the following order − 104A < 401A < M31-2A.


2016 ◽  
Vol 6 ◽  
pp. 104
Author(s):  
Víctor Pecina ◽  
Enrique Navarro ◽  
Héctor Williams ◽  
Raúl Rodríguez

The limited genetic variability of sorghum and the use of only one type of malesterility system (Milo-Kafir, cytoplasm A1) for the production hybrid seed, make this crop susceptible to diseases, thus its importance to look for new male sterility sources, as the cytoplasm A2 reported in 1977. This systems was introduced to the elite lines of the sorghum program at the Rio Bravo Experiment Station (INIFAP-CIRNE), in Tamaulipas, Mexico. The objective of this assay was to: a) compare the agronomic traits of two male-sterility systems (A1 and A2 cytoplasms), and b) determine if there are differences of the fertility restoration in the isocytoplasmic hybrids. The experimental design was a 7 x 7 lattice with four replications. The results indicate that there are no differences among the two male-sterility systems (Al and A2 cytoplasms) in grain yield, plant height and panicIe length; whereas in days to blooming. the A2 cytoplasm was a day late than the Al cytoplasm. Different restoration responses were found in the hybrids, as the R LRB-63 line which partially restored fertility in the two types of cytoplasms, while CS-3541 showed a similar response with the LRB-l02A, LRB-104A and LRB- 1l0A lines in the A2 cytoplasm.


Author(s):  
Muhammad Ahmad Yahaya ◽  
Hussein Shimelis ◽  
Mark Laing ◽  
Isack Mathew

A new generation of chemical hybridization agents (CHAs) or gametocides has shown potential to induce male sterility in predominantly self-fertilizing crops, including sorghum (Sorghum bicolor [L.] Moench). There is a lack of information on the relative efficacy of the various available CHAs for large-scale application in plant breeding programs. Therefore, the objective of the present study was to compare the relative effectiveness of three selected CHAs to induce male sterility in sorghum under a controlled environment for hybridization. Foliar applications of three CHAs and a control (ethrel, trifluoromethanesulfonamide [TFMSA], ethyl 4-fluorooxanilate [E4FO] and distilled water [control]) were tested using three grain sorghum genotypes (ICS-1, ICS-2 and ICS-3) in two seasons. The 24 treatment combinations consisting of 4 levels of CHAs, 3 sorghum varieties and two seasons were laid out using a randomized complete block design with three replications. Data on pollen sterility, pollen diameter, plant height, and panicle height were collected and analyzed. Results showed that the CHAs had significant (p<0.05) differences for efficacy of inducing male sterility in sorghum. Ethrel at a dose of 1 gl-1 induced the highest pollen sterility (98% in both seasons) but was highly phytotoxic with at least 60% mortality in the test population in both seasons, making it unsuitable for practical application. TFMSA (2 mg per plant) and E4FO (1 gl-1) d induced 93% male sterility with minimal phytotoxic effects (20 to 30%). Application of either TFMSA at 2mg per plant after flag leaf emergence or 1gl-1 of E4FO at panicle initiation can be used to successfully induce male sterility in sorghum under greenhouse conditions


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