A new human brain cDNA molecule: assignment to chromosome 11q21-q23.1 and description of two polymorphisms studied by the polymerase chain reaction

1993 ◽  
Vol 91 (2) ◽  
Author(s):  
Suzie Lefebvre ◽  
Jean-Fran�ois Bureau ◽  
Fran�oise Muscatelli ◽  
Marie-Genevi�ve Mattei ◽  
Michel Brahic
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Brain ◽  
Chain Reaction ◽  
Cdna Molecule ◽  
Polymerase Chain

scholarly journals Quantification of apolipoprotein E receptors in human brain-derived cell lines by real-time polymerase chain reaction

2005 ◽  
Vol 26 (6) ◽  
pp. 813-823 ◽  
Author(s):  
Shanaka Thilakawardhana ◽  
David M. Everett ◽  
Paul R. Murdock ◽  
Colin Dingwall ◽  
James S. Owen
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Brain ◽  
Apolipoprotein E ◽  
Real Time ◽  
Cell Lines ◽  
Chain Reaction ◽  
Polymerase Chain

Localization of HIV-1 in human brain using polymerase chain reaction/in situ hybridization and immunocytochemistry

1996 ◽  
Vol 39 (6) ◽  
pp. 705-711 ◽  
Author(s):  
Kiyomi Takahashi ◽  
Steven L. Wesselingh ◽  
Diane E. Griffin ◽  
Justin C. McArthur ◽  
Richard T. Johnson ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Human Brain ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Hiv 1

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

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