Isolation and partial characterization of the Asp-B10, Tyr-B25-des-(B26-B30)-proinsulin analog from inclusion bodies in Escherichia coli

1996 ◽  
Vol 10 (10) ◽  
Author(s):  
A.M.A. Nascimento ◽  
H.R.T. Souza ◽  
M.S. Xavier ◽  
J.E. Thiemann ◽  
L. Vilela ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Inclusion Bodies ◽  
Partial Characterization

scholarly journals Characterization of a Dye-Decolorizing Peroxidase from Irpex lacteus Expressed in Escherichia coli: An Enzyme with Wide Substrate Specificity Able to Transform Lignosulfonates

2021 ◽  
Vol 7 (5) ◽  
pp. 325
Author(s):  
Laura Isabel de de Eugenio ◽  
Rosa Peces-Pérez ◽  
Dolores Linde ◽  
Alicia Prieto ◽  
Jorge Barriuso ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Inclusion Bodies ◽  
Veratryl Alcohol ◽  
Dye Decolorization ◽  
Irpex Lacteus ◽  
Type D ◽  
Lignin Peroxidases

A dye-decolorizing peroxidase (DyP) from Irpex lacteus was cloned and heterologously expressed as inclusion bodies in Escherichia coli. The protein was purified in one chromatographic step after its in vitro activation. It was active on ABTS, 2,6-dimethoxyphenol (DMP), and anthraquinoid and azo dyes as reported for other fungal DyPs, but it was also able to oxidize Mn2+ (as manganese peroxidases and versatile peroxidases) and veratryl alcohol (VA) (as lignin peroxidases and versatile peroxidases). This corroborated that I. lacteus DyPs are the only enzymes able to oxidize high redox potential dyes, VA and Mn+2. Phylogenetic analysis grouped this enzyme with other type D-DyPs from basidiomycetes. In addition to its interest for dye decolorization, the results of the transformation of softwood and hardwood lignosulfonates suggest a putative biological role of this enzyme in the degradation of phenolic lignin.

Purification and partial characterization of coxsakievirus B3 2A protease expressed in Escherichia coli

2008 ◽  
Vol 43 (3) ◽  
pp. 238-244 ◽  
Author(s):  
Nader Maghsoudi ◽  
Fariba Khodagholi ◽  
Mahnaz Sadjadi ◽  
Mehdi Zeinodini ◽  
Marjan Sabbaghian
Keyword(s):  
Escherichia Coli ◽  
Partial Characterization ◽  
2A Protease

Isolation and partial characterization of Escherichia coli valine transfer RNA with uridine and uridine-derived residues replaced by 5-fluorouridine

1974 ◽  
Vol 88 (2) ◽  
pp. 301-312 ◽  
Author(s):  
Jack Horowitz ◽  
Ou Ching-Nan ◽  
Mohammad Ishaq ◽  
James Ofengand ◽  
Joan Bierbaum
Keyword(s):  
Escherichia Coli ◽  
Transfer Rna ◽  
Partial Characterization
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