Chloroplast and mitochondrial DNA composition of triploid and tetraploid somatic hybrids between Lycopersicon esculentum and Solanum tuberosum

1995 ◽  
Vol 90 (2) ◽  
pp. 285-293 ◽  
Author(s):  
A. M. A. Wolters ◽  
H. C. H. Schoenmakers ◽  
M. Koornneef
Keyword(s):  
Mitochondrial Dna ◽  
Solanum Tuberosum ◽  
Lycopersicon Esculentum ◽  
Somatic Hybrids

Chromosome pairing and potential for intergeneric recombination in some hypotetraploid somatic hybrids of Lycopersicon esculentum (+) Solanum tuberosum

Genome ◽  
1993 ◽  
Vol 36 (6) ◽  
pp. 1032-1041 ◽  
Author(s):  
J. H. de Jong ◽  
A. M. A. Wolters ◽  
J. M. Kok ◽  
H. Verhaar ◽  
J. van Eden
Keyword(s):  
Solanum Tuberosum ◽  
Lycopersicon Esculentum ◽  
Synaptonemal Complex ◽  
Chromosome Pairing ◽  
Somatic Hybrids ◽  
Cytogenetic Study ◽  
Unreduced Gametes ◽  
Root Tip ◽  
Prophase I ◽  
Metaphase I

Three somatic hybrids resulting from protoplast fusions of a diploid kanamycin-resistant line of tomato (Lycopersicon esculentum) and a dihaploid hygromycin-resistant transformant of a monohaploid potato (Solanum tuberosum) line were used for a cytogenetic study on chromosome pairing and meiotic recombination. Chromosome counts in root-tip meristem cells revealed two hypotetraploids with chromosome complements of 2n = 46 and one with 2n = 47. Electron microscope analyses of synaptonemal complex spreads of hypotonically burst protoplasts at mid prophase I showed abundant exchanges of pairing partners in multivalents involving as many as eight chromosomes. In the cells at late pachytene recombination nodules were found in multivalents on both sides of pairing partner exchanges, indicating recombination at both homologous and homoeologous sites. Light microscope observations of pollen mother cells at late diakinesis and metaphase I also revealed multivalents, though their occurrence in low frequencies betrays the reduction of multivalent number and complexity. Precocious separation of half bivalents at metaphase I and lagging of univalents at anaphase I were observed frequently. Bridges, which may result from an apparent inversion loop found in the synaptonemal complexes of a mid prophase I nucleus, were also quite common at anaphase I, though the expected accompanying fragments could be detected in only a few cells. Most striking were the high frequencies of first division restitution in preparations at metaphase II/anaphase II, giving rise to unreduced gametes. In spite of the expected high numbers of balanced haploid and diploid gametes, male fertility, as revealed by pollen staining, was found to be negligible.Key words: synaptonemal complex, recombination, chromosome pairing, somatic hybrid, Lycopersicon esculentum (+) Solanum tuberosum.

Mitochondrial DNA rearrangements in somatic hybrids of Solanum tuberosum and Solanum brevidens

1986 ◽  
Vol 72 (6) ◽  
pp. 787-793 ◽  
Author(s):  
R. J. Kemble ◽  
T. L. Barsby ◽  
R. S. C. Wong ◽  
J. F. Shepard
Keyword(s):  
Mitochondrial Dna ◽  
Solanum Tuberosum ◽  
Somatic Hybrids ◽  
Solanum Brevidens ◽  
Dna Rearrangements

Allotriploid somatic hybrids of diploid tomato (Lycopersicon esculentum Mill.) and monoploid potato (Solanum tuberosum L.)

1993 ◽  
Vol 87 (3) ◽  
pp. 328-336 ◽  
Author(s):  
H. C. H. Schoenmakers ◽  
A. M. A. Wolters ◽  
E. M. Nobel ◽  
C. M. J. de Klein ◽  
M. Koornneef
Keyword(s):  
Solanum Tuberosum ◽  
Lycopersicon Esculentum ◽  
Somatic Hybrids ◽  
Solanum Tuberosum L ◽  
Lycopersicon Esculentum Mill

scholarly journals Determinación de Razas Fisiológicas y Tipo de Apareamiento en Aislamientos de Phytophthora infestans (Mont) De Bary

2016 ◽  
Vol 9 (1) ◽  
pp. 156-170
Author(s):  
Juan Bautista López ◽  
María Elena Márquez ◽  
Sonia Jaramillo ◽  
José Luís Zapata
Keyword(s):  
Solanum Tuberosum ◽  
Lycopersicon Esculentum ◽  
Phytophthora Infestans ◽  
Solanum Phureja ◽  
Solanum Muricatum

El problema más limitante del cultivo de la papa en muchas regiones del mundo, lo constituye la gota o tizón tardío ocasionado por el hongo Phytophthora infestans. Esta investigación se realizó con el objetivo de determinar variaciones poblacionales de este patógeno, basadas en el tipo de apareamiento y en la presencia de razas fisiológicas en 19 aislamientos de P. infestans obtenidos en plantas de papa (Solanum tuberosum) variedades Diacol Capiro, ICA Puracé e ICA Cumanday; papa criolla (Solanum phureja), pepino (Solanum muricatum) y tomate (Lycopersicon esculentum) en diferentes zonas del Departamento de Antioquia (Colombia).Los resultados obtenidos a partir de un apareamiento patrón A1, con los aislamientos colectados en las diferentes zonas sugieren la existencia de una población de P. infestans con un solo tipo de apareamiento. La prueba de virulencia del hongo, realizada en folíolos de 11 diferenciales de papa mostró la presencia de 6 razas fisiológicas, de las cuales la más frecuente fue la 7446 (58%), con los factores de virulencia 1-2-3-4-7-10-11. Aislamientos monozoospóricos presentaron los mismos factores de virulencia que sus respectivos aislamientos recolectados en el campo, lo que sugiere homogeneidad de la población fungosa. La gran complejidad de las razas fisiológicas de P. infestans podría estar influenciada por diversos factores genéticos y medioambientales.Aceptado para publicación: mayo 1997.

Somatic hybrids between potato Solanum tuberosum and wild species Solanum verneï exhibit a recombination in the plastome

2005 ◽  
Vol 83 (1) ◽  
pp. 1-11 ◽  
Author(s):  
S. Trabelsi ◽  
R. Gargouri-Bouzid ◽  
F. Vedel ◽  
A. Nato ◽  
L. Lakhoua ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Wild Species ◽  
Somatic Hybrids ◽  
Solanum Vernei
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