Histochemical study of the localization of cholinesterases in the central nervous system of Sepia officinalis

1974 ◽  
Vol 148 (4) ◽  
Author(s):  
M.P. Chichery ◽  
R. Chichery
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Histochemical Study ◽  
Sepia Officinalis ◽  
The Central Nervous System

Peptidergic regulation of chromatophore function in the European cuttlefish Sepia officinalis

1996 ◽  
Vol 199 (5) ◽  
pp. 1177-1187 ◽  
Author(s):  
P Loi ◽  
R Saunders ◽  
D Young ◽  
N Tublitz
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Neuromuscular Control ◽  
Sepia Officinalis ◽  
Close Apposition ◽  
Immunoblot Assay ◽  
The Central Nervous System ◽  
The Brain ◽  
Excitatory Transmitter

Color patterning in cephalopod molluscs involves activation of a peripheral chromatophore system that is under neuromuscular control. The complex behavior of individual chromatophores is mediated by a specific set of muscles, the chromatophore muscles, that receive direct innervation from the central nervous system. To date, glutamate is the only excitatory transmitter that has been proposed to act at the chromatophore neuromuscular junction of cephalopods. We present data demonstrating that the chromatophore muscles in the European cuttlefish Sepia officinalis are also regulated by the FMRFamide family of neuropeptides. Using an in vitro chromatophore bioassay, it has been determined that several FMRFamide-related peptides (FaRPs) are potent excitors of the chromatophore muscles, causing chromatophore expansion. Immunocytochemical analyses of the central nervous system using an FMRFamide antibody revealed the presence of FMRFamide-like immunoreactive cell bodies in the posterior chromatophore lobes, the region of the brain containing the chromatophore motoneurons of the fin and mantle. FMRFamide-like immunoreactivity was also seen in the periphery, in the nerves around the chromatophores and in close apposition to the muscles in the chromatophore layer of the fin. HPLC analysis of the fin dermis isolated four bioactive peaks that were FMRFamide-immunoreactive when tested on an immunoblot assay. Two of these peaks co-eluted with known FaRPs, FMRFamide and ALSGDAFLRFamide, a decapeptide isolated from squid. Taken together, these data suggest that the FaRPs are likely to be endogenous excitors of the chromatophore muscles in cephalopods.

Nitric oxide synthase expression in the central nervous system of Sepia officinalis: an in situ hybridization study

2007 ◽  
Vol 26 (6) ◽  
pp. 1599-1610 ◽  
Author(s):  
Carlo Di Cristo ◽  
Gabriella Fiore ◽  
Vladimir Scheinker ◽  
Grigori Enikolopov ◽  
Marco D'Ischia ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Central Nervous System ◽  
Nervous System ◽  
In Situ Hybridization ◽  
Nitric Oxide Synthase ◽  
Sepia Officinalis ◽  
Hybridization Study ◽  
The Central Nervous System ◽  
Oxide Synthase
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