Production and cytogenetics of intergeneric hybrids between Brassica napus and Orychophragmus violaceus

1995 ◽  
Vol 91 (1) ◽  
pp. 131-136 ◽  
Author(s):  
Z. Li ◽  
H. L. Liu ◽  
P. Luo
Keyword(s):  
Brassica Napus ◽  
Intergeneric Hybrids ◽  
Orychophragmus Violaceus

Cytogenetic and molecular characterization of intergeneric hybrids between Brassica napus and Orychophragmus violaceus

Genome ◽  
2002 ◽  
Vol 45 (1) ◽  
pp. 110-115 ◽  
Author(s):  
B F Cheng ◽  
G Séguin-Swartz ◽  
D J Somers
Keyword(s):  
Brassica Napus ◽  
Morphological Traits ◽  
Embryo Rescue ◽  
Chromosome Elimination ◽  
Intergeneric Hybrids ◽  
Intergeneric Hybridization ◽  
Orychophragmus Violaceus ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Twenty-two intergeneric hybrids from a cross between Brassica napus (AACC, 2n = 38) cultivar Oro and the ornamental crucifer Orychophragmus violaceus (OO, 2n = 24) were produced without embryo rescue. The plants were classified into three groups based on morphological and cytological observations and RAPD banding patterns. Plants of Group I had morphological traits of both parents and 2n = 29 chromosomes. In these plants, 62.1% of the pollen mother cells (PMCs) had the pairing configuration 1 III + 9 II + 8 I; the remaining PMCs had 10 II + 9 I. The plants possessed 97.6–98.8% B. napus specific and 9.2–11.7% O. violaceus specific RAPD fragments. Plants of Group II exhibited novel morphological traits and possessed 2n = 35, 36, or 37 chromosomes. Plants of Group III were morphologically similar to B. napus and possessed 2n = 19, 37, 38, or 39 chromosomes. Plants of Group II and Group III had 94.1–99.4% B. napus specific RAPD fragments and no O. violaceus specific RAPD fragments. Chromosome fragments were observed in PMCs of most of the F1 plants in all groups. Based on the cytological results and RAPD analysis, it is suggested that genome doubling and chromosome elimination occurred in the intergeneric hybrids of B. napus × O. violaceus.Key words: Brassica, intergeneric hybridization, meiosis, RAPD, chromosome elimination.

Alteration of chromosome behavior and synchronization of parental chromosomes after successive generations in Brassica napus × Orychophragmus violaceus hybrids

Genome ◽  
2007 ◽  
Vol 50 (2) ◽  
pp. 226-233 ◽  
Author(s):  
Zhigang Zhao ◽  
Ni Ma ◽  
Zaiyun Li
Keyword(s):  
Brassica Napus ◽  
Meiotic Division ◽  
Morphological Characteristics ◽  
Intergeneric Hybrids ◽  
Orychophragmus Violaceus ◽  
Genome Separation ◽  
Cytogenetic Evidence ◽  
Successive Generations ◽  
Mother Cells

In an earlier study, the progenies of intergeneric hybrids Brassica napus (2n = 38) × Orychophragmus violaceus (2n = 24) were investigated in successive generations (F1–F4) for the cytological phenomenon of parental genome separation during mitotic and meiotic division. In the present study, inbred lines (F5–F8) derived from 1 such hybrid were characterized for morphology, chromosome pairing behaviour, and genome composition. One F5 plant (2n = 31) with slightly yellow petals and 12:19 and 15:16 segregation ratios in its pollen mother cells (PMCs) produced F6 plants with distinct morphological characteristics and wide variations in fertility and chromosome numbers (2n = 25–38). F7 and F8 lines with distinctive morphology and wide ranges in chromsome numbers were established. In PMCs of F7 plants from 4 F6 plants, 0–12 labelled chromosomes from O. violaceus, which predominantly appeared as bivalents, were identified by genomic in situ hybridization. They behaved synchronously with B. napus chromosomes during meiotic division. The results provide molecular cytogenetic evidence of the inclusion of O. violaceus chromosomes in the original hybrids and the cytology in the hybrids documented earlier. They also show that chromosome behaviour was altered and the parental chromosomes became synchronized after successive generations.

scholarly journals Comparison on the Nutrient Plunder Capacity of Orychophragmus violaceus and Brassica napus L. Based on Electrophysiological Information

Horticulturae ◽  
2021 ◽  
Vol 7 (8) ◽  
pp. 206
Author(s):  
Cheng Zhang ◽  
Yue Su ◽  
Yanyou Wu ◽  
Haitao Li ◽  
Ying Zhou ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Orychophragmus Violaceus ◽  
Nutrient Metabolism ◽  
Brassica Napus L ◽  
Adaptive Mechanisms ◽  
Capacitive Reactance ◽  
Time Determination ◽  
Almost All ◽  
First Time

The nutrient metabolism, growth and development of plants are strongly affected by its nutrient plunder, and plants have different adaptive mechanisms to low-nutrient environments. The electrophysiological activities involve almost all life processes of plants. In this study, the active transport flow of nutrient (NAF) and nutrient plunder capacity (NPC) of plants were defined based on leaf intrinsic impedance (IZ), capacitive reactance (IXc), inductive reactance (IXL) and capacitance (IC) to evaluate the nutrient plunder capacity of plants for the first time. The results indicate that Orychophragmus violaceus had higher (p < 0.01) NPC and IC and lower (p < 0.01) IR, IXc, IXL and IZ as compared to Brassica napus L., which supports a superior ion affinity and that it could be better adapted to low-nutrient environments. UAF and NPC of plants exhibited good correlations with crude protein, crude ash and water content, and precisely revealed the plunder capacity and adaptive strategies of plants to nutrients. The present work highlights that O. violaceus had superior NPC and ion affinity compared with B. napus, and provided a novel, rapid, reliable method based on the plant’s electrophysiological information for real-time determination of the nutrient plunder capacity of plants.

Production and characterization of intergeneric somatic hybrids between Brassica napus and Orychophragmus violaceus and their backcrossing progenies

2008 ◽  
Vol 27 (10) ◽  
pp. 1611-1621 ◽  
Author(s):  
Zhi-gang Zhao ◽  
Ting-ting Hu ◽  
Xian-Hong Ge ◽  
Xue-zhu Du ◽  
Li Ding ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Somatic Hybrids ◽  
Orychophragmus Violaceus
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