Stereoscopic scanning electron microscopy of the red pulp of dog spleen with special reference to the terminal structure of the cordal capillaries

1977 ◽  
Vol 182 (4) ◽  
Author(s):  
Teruo Suzuki ◽  
Masakuni Furusato ◽  
Satoshi Takasaki ◽  
Susumu Shimizu ◽  
Yoshiaki Hataba
2020 ◽  
Vol 83 (9) ◽  
pp. 1066-1078 ◽  
Author(s):  
Tahira Alyas ◽  
Shabnum Shaheen ◽  
Uswah Amber ◽  
Nidaa Harun ◽  
Sana Khalid ◽  
...  

Author(s):  
S. P. Hopkin ◽  
J. A. Nott

Aspects of the digestive cycle of Carcinus maenas have been studied by transmission and scanning electron microscopy, X-ray microanalysis and light microscopy. Movements of ingested material have been followed by feeding the crabs with Mytilus edulis (L.) labelled with colloidal particles of gold or thorium oxide.


Author(s):  
Teruo Suzuki ◽  
Susumu Shimizu ◽  
Yoshiaki Hataba ◽  
Yuji Kirino

Introduction In spite of the efforts of many researchers, three dimensional fine structure of the capillary terminals in the red pulp of the spleen has not been successively demonstrated up to date. By stereoscopic scanning electron microscopy of a perfusion-fixed and freeze-fractured dog spleen, we have recently demonstrated that the terminals of the cordal capillaries are obviously open in the cordal labyrinth.Material and Methods In the human spleen, however, applications of the same method only was not necessarily successful to obtain unquestionable informations on their three dimensional structure, since it is very difficult to flush out blood cells thoroughly from the cordal labyrinth at the time of the perfusion fixation. In the present study, a synthetical observation of the capillary terminals in the red pulp of the normal human spleen was undertaken by three ways of; 1) transmission electron microscopy of ultrathin sections (TEM), 2) scanning electron microscopy of freeze-fractured tissue (FF-SEM), and 3) scanning electron microscopy of serial sections from which the embedding medium was removed (SS-SEM).


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