Regeneration of intergeneric somatic hybrid plants between Lycopersicon esculentum and Solanum muricatum

1991 ◽  
Vol 81 (4) ◽  
pp. 509-513 ◽  
Author(s):  
K. Sakomoto ◽  
T. Taguchi
Keyword(s):  
Lycopersicon Esculentum ◽  
Somatic Hybrid ◽  
Solanum Muricatum ◽  
Hybrid Plants ◽  
Intergeneric Somatic Hybrid

Somatic hybrid plants between Lycopersicon esculentum and Solanum lycopersicoides

1986 ◽  
Vol 71 (5) ◽  
pp. 691-697 ◽  
Author(s):  
L. W. Handley ◽  
R. L. Nickels ◽  
M. W. Cameron ◽  
P. P. Moore ◽  
K. C. Sink
Keyword(s):  
Lycopersicon Esculentum ◽  
Somatic Hybrid ◽  
Solanum Lycopersicoides ◽  
Hybrid Plants

Asymmetric Somatic Hybridization: Developing a Gene Transfer System for Solanaceous Vegetable Crops

1996 ◽  
Author(s):  
Ken Sink ◽  
Shamay Izhar ◽  
Abraham Nachmias
Keyword(s):  
Lycopersicon Esculentum ◽  
Somatic Hybrid ◽  
Blot Hybridization ◽  
Rflp Analysis ◽  
Kanamycin Resistance ◽  
Nptii Gene ◽  
Vegetable Crops ◽  
Dot Blot ◽  
Hybrid Plants ◽  
Gamma Irradiated

Highly asymmetric somatic hybrid plants were obtained by PEG/DMSO fusion of gamma irradiated (100, 250, 7500 and 1000 Gy) protoplasts of a (KmR-) interspecific hybrid Lycopersicon esculentum x L. pennellii (EP) with protoplasts of eggplant (E). Somatic hybrid calli were selected based on kanamycin resistance and verified by PCR of the NptII gene, RAPD's and Southern's using potato rDNA pTHG2 probes. Flow cytometry indicated all hybrid calli that did not regenerate shoots were 5-9n. Three asymmetric plants regenerated only from callus close to 4n and such calli oly occurred when EP received 100 Gy. The asymmetric plants had eggplant morphology and regenerated from one hybrid callus with 6.29 average size tomato chromosomes. Limited amounts of EP DNA were found in the three somatic hybrid plants H18-1 to -3 by dot-blot hybridization with probe pTHG2, to be equivalent to 6.23, 5.41, and 5.95 % EP, respectively. RFLP analysis of Lycopersicon esculentum and L. pennellii specific chromosomes revealed that only fragments of 8 to 10 out of the 24 EP chromosomes are present in the asymmetric plants. Transgenic plants 2-3, 2-4 and 10-3 were found resistant to verticillium; suggesting successful transfer of the Ve complex from S. torvum to eggplant.

Somatic Hybrid Plants of Lycopersicon esculentum Mill.and Lycopersicon peruvianum Mill.

1986 ◽  
Vol 125 (3-4) ◽  
pp. 225-234 ◽  
Author(s):  
A. Kinsara ◽  
S.N. Patnaik ◽  
E.C. Cocking ◽  
J.B. Power
Keyword(s):  
Lycopersicon Esculentum ◽  
Somatic Hybrid ◽  
Lycopersicon Peruvianum ◽  
Hybrid Plants
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