Electron microscopic immunocytochemical demonstration of separate mesotocinergic and vasotocinergic nerve fibres in the pars intermedia of the amphibian hypophysis

1977 ◽  
Vol 178 (2) ◽  
pp. 175-181 ◽  
Author(s):  
A. Van Vossel ◽  
J. Van Vossel-Daeninck ◽  
K. Dierickx ◽  
F. Vandesande
Keyword(s):  
Pars Intermedia ◽  
Nerve Fibres ◽  
Electron Microscopic

scholarly journals The growth rate of sensory nerve fibres in the mammalian embryo

Development ◽  
1987 ◽  
Vol 100 (2) ◽  
pp. 307-311 ◽  
Author(s):  
A.M. Davies
Keyword(s):  
Sensory Nerve ◽  
Rate Of Change ◽  
Linear Functions ◽  
Nerve Fibres ◽  
Electron Microscopic ◽  
Mammalian Embryo ◽  
Maxillary Nerve ◽  
Fibre Number ◽  
Two Parameters ◽  
Electron Microscopic Method

I have used a novel quantitative electron microscopic method to determine the rate at which nerve fibres grow towards their targets during development. The rate of recruitment of nerve fibres to the maxillary nerve of the mouse embryo was determined by counting the number of axon profiles in the nerve sectioned close to its emergence from the trigeminal ganglion at closely staged intervals throughout its early development. The rate of change of fibre number with distance along this nerve was determined by counting the number of axon profiles at intervals along the nerve at stages during the period that fibres are growing to their targets. From these two parameters, both of which were linear functions during the midperiod of fibre recruitment to the nerve, it has been possible to calculate that embryonic sensory nerve fibres grow to their peripheral targets at the surprisingly slow rate of just over 20 micron h-1.

Detection of Noradrenaline-Immunoreactive Nerve Fibres in Rat Liver by Immunoelectron Microscopy

1997 ◽  
Vol 25 (6) ◽  
pp. 354-358 ◽  
Author(s):  
Y Fukuda ◽  
M Imoto ◽  
I Nakano ◽  
Y Katano ◽  
T Hayakawa
Keyword(s):  
Portal Vein ◽  
Rat Liver ◽  
Close Contact ◽  
Portal Tract ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Nerve Fibres ◽  
Electron Microscopic ◽  
Noradrenergic Innervation ◽  
Preliminary Study

Noradrenergic innervation of rat liver was studied immunohistochemically using antibody to noradrenaline at the electron-microscopic level. Noradrenaline-immunoreactive nerve fibres were located in the portal tract and some were in close contact with the portal vein and hepatic artery. Noradrenaline-immunoreactive fibres were found to contain many vesicles that were reactive to anti-noradrenaline antibody. This preliminary study suggests that the method for detecting noradrenaline-immunoreactive fibres using the antibody is useful for studies at the electron-microscopic level.

scholarly journals Immunocytochemical evidence for vasopressin receptors.

1978 ◽  
Vol 26 (7) ◽  
pp. 581-592 ◽  
Author(s):  
M Castel
Keyword(s):  
Solid Phase ◽  
Secretory Granules ◽  
Cell Types ◽  
Staining Intensity ◽  
Pars Intermedia ◽  
Electron Microscopic ◽  
Neural Lobe ◽  
Acth Cell ◽  
Receptor Sites ◽  
Vasopressin Receptors

An electron microscopic study was made of mouse pituitaries immunocytochemically stained with anti-lysine vasopressin (LVP) as the primary antiserum in the unlabeled antibody peroxidase-anti-peroxidase procedure. Vasopressin (VP) was identified in the neurosecretory granules of the neural lobe which stained with peroxidase anti-peroxidase molecules. Electron density was induced in secretory granules of the pars intermedia (PI), both in the melanocyte stimulated hormone and ACTH cell types, probably indicating VP molecules attached to binding (receptor) sites. Omission of anti-LVP abolished staining both in the neural lobe and the PL Anti-LVP absorbed with antigen, by admixing with LVP, abolished staining in the neural lobe but not in the PI; according to optical density measurements the PI showed a +/- 22% staining increase over controls. Staining intensity in the PI probably reflects occupancy of binding (receptor) sites for VP. Exposure of PI granules to LVP before the usual staining sequence resulted in +/- 48% increased staining. In water-deprived mice with high endogenous VP titers, staining was +/- 33% and +/- 40% more intense than in normal mice. Solid phase absorbed and eluted antibodies to LVP provided additional proof that staining in both neural lobe and PI could be attributed to anti-LVP. Results indicate that binding or receptor sites for VP are located on secretory granules in the PL Possible physiological significance is discussed.

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