Control of peptide release from cells of the intermediate lobe of the rat pituitary

1984 ◽  
Vol 236 (3) ◽  
Author(s):  
Guido Stoll ◽  
Rainer Martin ◽  
Karl-H. Voigt
Keyword(s):  
Intermediate Lobe ◽  
Peptide Release ◽  
Rat Pituitary

The D-2 Dopamine Receptor in the Intermediate Lobe of the Rat Pituitary Gland

1983 ◽  
pp. 33-72 ◽  
Author(s):  
J. W. KEBABIAN ◽  
M. BEAULIEU ◽  
T. E. COTE ◽  
R. L. ESKAY ◽  
E. A. FREY ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Dopamine Receptor ◽  
Intermediate Lobe ◽  
Rat Pituitary

scholarly journals Immunocytochemical demonstration of tissue-type plasminogen activator in endocrine cells of the rat pituitary gland.

1985 ◽  
Vol 101 (1) ◽  
pp. 305-311 ◽  
Author(s):  
P Kristensen ◽  
L S Nielsen ◽  
J Grøndahl-Hansen ◽  
P B Andresen ◽  
L I Larsson ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Pituitary Gland ◽  
Endocrine Cells ◽  
Large Population ◽  
Cell Types ◽  
Anterior Lobe ◽  
Tissue Type ◽  
Intermediate Lobe ◽  
Posterior Lobe ◽  
Rat Pituitary

We immunocytochemically stained rat pituitary glands using antibodies against plasminogen activators of the tissue type (t-PA) and the urokinase type (u-PA). A large population of endocrine cells in the anterior lobe of the gland displayed intense cytoplasmic immunoreactivity with anti-t-PA. In some areas of the intermediate lobe we found a weak staining, and we observed weakly staining granular structures in the posterior lobe. Controls included absorption of the antibodies with highly purified t-PA. In addition, SDS PAGE followed by immunoblotting of pituitary gland extracts revealed only one band with an electrophoretic mobility similar to that of t-PA when stained with anti-t-PA IgG. No u-PA immunoreactivity was detected in the rat pituitary gland. Sequential staining experiments using antibodies against growth hormone and t-PA demonstrated that the t-PA-immunoreactive cells constitute a large subpopulation of the growth hormone-containing cells. These findings represent the first direct evidence for the presence of t-PA in cell types other than endothelial cells in the intact normal organism. In this article we discuss the implications of the results for a possible role of t-PA in the posttranslational processing of prohormones.

Inhibition by prostaglandin E2of the release of vasopressin and β-endorphin from rat pituitary neurointermediate lobe or medial basal hypothalamus in vitro

1985 ◽  
Vol 106 (2) ◽  
pp. 189-195 ◽  
Author(s):  
W. Knepel ◽  
D. Nutto ◽  
M. Vlaskovska ◽  
Ch. Kittel
Keyword(s):  
Direct Action ◽  
Effective Concentration ◽  
Neurosecretory System ◽  
Intermediate Lobe ◽  
Medial Basal Hypothalamus ◽  
Neurointermediate Lobe ◽  
Rat Pituitary ◽  
Prostaglandin F ◽  
Spontaneous Secretion

ABSTRACT The present study was performed to examine the effect of the cyclo-oxygenase inhibitor, indomethacin, and that of various prostaglandins on the release of vasopressin and β-endorphin-like immunoreactivity (β-EI) from the rat neurointermediate lobe of the hypophysis, which was superfused in vitro. Indomethacin (2·8 and 28 μmol/l) changed neither basal secretion of vasopressin nor that evoked by electrical stimulation, whereas the resting release of β-EI was enhanced by indomethacin (28 μmol/l). Prostaglandin (PG) E2 did not influence resting release of vasopressin but markedly inhibited (by about 50%) electrically induced release of vasopressin (least effective concentration: 300 nmol/l) as well as spontaneous secretion of β-EI (least effective concentration: 100 nmol/l) in the presence of indomethacin (28 μmol/l). Prostaglandin F2α (5 μmol/l) also inhibited the evoked release of vasopressin, whereas PGD2 (5 μmol/l) did not. Prostaglandin F2α (5 μmol/l), D2 and I2 (1·5 μmol/l each) produced no effects on β-EI release. As observed in the neurohypophysis, PGE2 inhibited the electrically induced release of vasopressin from the medial basal hypothalamus in vitro. We conclude that prostaglandins (especially PGE2) can inhibit (1) the stimulated release of vasopressin when acting on vasopressin-containing nerve terminals of either neurosecretory system (neurohypophysis, median eminence region), and (2) the secretion of β-EI and, as can be inferred, α-MSH, by a direct action on intermediate lobe cells. J. Endocr. (1985) 106, 189–195

Bromocriptine-Induced Changes in the Biochemistry, Physiology, and Histology of the Intermediate Lobe of the Rat Pituitary Gland

Endocrinology ◽  
1984 ◽  
Vol 114 (5) ◽  
pp. 1871-1884 ◽  
Author(s):  
M. BEAULIEU ◽  
M. E. GOLDMAN ◽  
K. MIYAZAKI ◽  
E. A. FREY ◽  
R. L. ESKAY ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Intermediate Lobe ◽  
Rat Pituitary ◽  
Induced Changes

Dopamine D2 receptor stimulation alters G-protein expression in rat pituitary intermediate lobe melanotropes

Endocrine ◽  
1997 ◽  
Vol 6 (3) ◽  
pp. 325-333 ◽  
Author(s):  
Scott A. Sands ◽  
Daniel S. Dickerson ◽  
Stephen J. Morris ◽  
Bibie M. Chronwall
Keyword(s):  
Protein Expression ◽  
G Protein ◽  
Dopamine D2 Receptor ◽  
D2 Receptor ◽  
Intermediate Lobe ◽  
Receptor Stimulation ◽  
Dopamine D2 ◽  
Rat Pituitary ◽  
Pituitary Intermediate Lobe
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