Immunohistochemical study of atrial natriuretic polypeptides in the embryonic, fetal and neonatal rat heart

1987 ◽  
Vol 248 (3) ◽  
pp. 627-633 ◽  
Author(s):  
Hirotaka Toshimori ◽  
Kiyotaka Toshimori ◽  
Chikayoshi Ōura ◽  
Hisayuki Matsuo
Keyword(s):  
Rat Heart ◽  
Immunohistochemical Study ◽  
Neonatal Rat ◽  
Neonatal Rat Heart ◽  
Atrial Natriuretic

Changes in expression of a functional Gi protein in cultured rat heart cells

1988 ◽  
Vol 255 (1) ◽  
pp. C51-C59 ◽  
Author(s):  
I. S. Allen ◽  
S. T. Gaa ◽  
T. B. Rogers
Keyword(s):  
Adenylate Cyclase ◽  
Pertussis Toxin ◽  
Rat Heart ◽  
Neonatal Rat ◽  
Heart Cells ◽  
Dependent Manner ◽  
Camp Accumulation ◽  
Ang Ii ◽  
Rat Heart Myocytes ◽  
Neonatal Rat Heart

The muscarinic cholinergic agonist, carbachol, and pertussis toxin were used to examine the functional status of the guanine nucleotide-binding protein that inhibits adenylate cyclase (Gi) in cultured neonatal rat heart myocytes. The isoproterenol stimulation of adenylate cyclase activity in myocyte membranes and adenosine 3',5'-cyclic monophosphate (cAMP) accumulation in intact cells (4 days in culture) were insensitive to carbachol (0.1 mM). However, in cells cultured for 11 days, carbachol (0.1 mM) inhibited isoproterenol-stimulated cAMP accumulation by 30%. Angiotensin II (ANG II) was also found to inhibit isoproterenol-stimulated cAMP accumulation in day 11 cells in a dose-dependent manner. Pertussis toxin treatment reversed the inhibitory effects of both ANG II and carbachol, suggesting a role for Gi in the process. Carbachol binding to membranes from day 4 cells was relatively insensitive to guanine nucleotides when compared with binding to membranes from day 11 or adult cells. Furthermore, pertussis toxin-mediated 32P incorporation into a 39- to 41-kDa substrate in day 11 membranes was increased 3.2-fold over that measured in day 4 membranes. These findings support the view that, although Gi is expressed, it is nonfunctional in 4-day-old cultured neonatal rat heart myocytes and acquisition of functional Gi is dependent on culture conditions. Furthermore, the ANG II receptor can couple to Gi in heart.

Chronotropic effect of histamine on cultured neonatal rat heart cells

1979 ◽  
Vol 58 (2) ◽  
pp. 117-123 ◽  
Author(s):  
Klara Csete ◽  
Marie-Claude Auclair ◽  
Paul Lechat
Keyword(s):  
Rat Heart ◽  
Neonatal Rat ◽  
Heart Cells ◽  
Chronotropic Effect ◽  
Neonatal Rat Heart ◽  
Rat Heart Cells

scholarly journals Angiotensin Converting Enzyme Inhibition Decreases Cell Turnover in the Neonatal Rat Heart

2002 ◽  
Vol 52 (3) ◽  
pp. 325-332 ◽  
Author(s):  
Jeong Hoon Choi ◽  
Kee Hwan Yoo ◽  
Hae Won Cheon ◽  
Kyung Burm Kim ◽  
Young Sook Hong ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Enzyme Inhibition ◽  
Rat Heart ◽  
Neonatal Rat ◽  
Angiotensin Converting Enzyme Inhibition ◽  
Converting Enzyme ◽  
Cell Turnover ◽  
Converting Enzyme Inhibition ◽  
Neonatal Rat Heart
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