Preimplantation mouse embryos express Mhc class I genes before the first cleavage division

1993 ◽  
Vol 38 (1) ◽  
pp. 35-40 ◽  
Author(s):  
MatthewT. Sprinks ◽  
MartinH. Sellens ◽  
GillianB. Dealtry ◽  
Nelson Fernandez
Keyword(s):  
Mhc Class I ◽  
Mouse Embryos ◽  
Class I ◽  
Cleavage Division ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse

scholarly journals Uptake and Incorporation of Glucose especially into the Glycogen Pools of Preimplantation Mouse Embryos during Culture in vitro

1982 ◽  
Vol 35 (2) ◽  
pp. 195 ◽  
Author(s):  
I L Pike ◽  
RG Wales
Keyword(s):  
Glucose Metabolism ◽  
Mouse Embryos ◽  
Cleavage Division ◽  
The Third ◽  
Preimplantation Mouse Embryos ◽  
Initial Cleavage ◽  
Preimplantation Mouse ◽  
Concentration Changes ◽  
Lactate And Pyruvate

Rate of [14C]glucose uptake by mouse embryos during in vitro culture in media containing optimal concentrations of lactate and pyruvate increased throughout preimplantation development. A reduction in the glucose concentration of the medium from 5�56 to 0�28 mM resulted in a two- to fivefold decrease in glucose metabolism, suggesting near-saturation of the membrane carrier at the lower concentration. Changes in the level of lactate and pyruvate in the medium had little effect on glucose metabolism after the third cleavage division. However, further evidence of interaction between energy substrates during the initial cleavage was obtained.

Human Adenovirus Uptake by Preirnplantation Mouse Embryos

1972 ◽  
Vol 30 ◽  
pp. 268-269
Author(s):  
D. G. Chase ◽  
W. Winters ◽  
L. Piko
Keyword(s):  
Hela Cells ◽  
Human Adenovirus ◽  
Mouse Embryos ◽  
Equilibrium Density ◽  
Cell Stage ◽  
Virus Attachment ◽  
Preimplantation Mouse Embryos ◽  
Embryo Culture Medium ◽  
Preimplantation Mouse ◽  
Mouse Cells

Although the outlines of human adenovirus entry and uncoating in HeLa cells has been clarified in recent electron microscope studies, several details remain unclear or controversial. Furthermore, morphological features of early interactions of human adenovirus with non-permissive mouse cells have not been extensively documented. In the course of studies on the effects of human adenoviruses type 5 (AD-5) and type 12 on cultured preimplantation mouse embryos we have examined virus attachment, entry and uncoating. Here we present the ultrastructural findings for AD-5.AD-5 was grown in HeLa cells and purified by successive velocity gradient and equilibrium density gradient centrifugations in CsCl. After dialysis against PBS, virus was sedimented and resuspended in embryo culture medium. Embryos were placed in culture at the 2-cell stage in Brinster's medium.

Localization of Intracisternal a Particle Antigens in Neoplastic Cells and Preimplantation Mouse Embryos

1980 ◽  
Vol 38 ◽  
pp. 696-697
Author(s):  
Thomas T.F. Huang ◽  
Patricia G. Calarco
Keyword(s):  
Endoplasmic Reticulum ◽  
Normal Development ◽  
Mouse Embryos ◽  
Neoplastic Cells ◽  
Large Numbers ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse ◽  
Intracisternal A Particle ◽  
Normal Feature

The stage specific appearance of a retravirus, termed the Intracisternal A particle (IAP) is a normal feature of early preimplantation development. To date, all feral and laboratory strains of Mus musculus and even Asian species such as Mus cervicolor and Mus pahari express the particles during the 2-8 cell stages. IAP form by budding into the endoplasmic reticulum and appear singly or as groups of donut-shaped particles within the cisternae (fig. 1). IAP are also produced in large numbers in several neoplastic cells such as certain plasmacytomas and rhabdomyosarcomas. The role of IAP, either in normal development or in neoplastic behavior, is unknown.

Effect of donor bovine serum fractionation on preimplantation mouse embryos in culture

1994 ◽  
Vol 41 (1) ◽  
pp. 257 ◽  
Author(s):  
C.N. Millham ◽  
M.B. Tornesi ◽  
A.T. Palasz ◽  
J. Archer
Keyword(s):  
Bovine Serum ◽  
Mouse Embryos ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse ◽  
Serum Fractionation
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