Insertional mutagenesis in Arabidopsis thaliana: isolation of a T-DNA-linked mutation that alters leaf morphology

1991 ◽  
Vol 81 (2) ◽  
pp. 277-284 ◽  
Author(s):  
M. Van Lijsebettens ◽  
R. Vanderhaeghen ◽  
M. Van Montagu
Keyword(s):  
Arabidopsis Thaliana ◽  
Leaf Morphology ◽  
Insertional Mutagenesis

scholarly journals Mutants in Arabidopsis thaliana Altered in Epicuticular Wax and Leaf Morphology

1996 ◽  
Vol 110 (2) ◽  
pp. 377-385 ◽  
Author(s):  
M. A. Jenks ◽  
A. M. Rashotte ◽  
H. A. Tuttle ◽  
K. A. Feldmann
Keyword(s):  
Arabidopsis Thaliana ◽  
Leaf Morphology ◽  
Epicuticular Wax

Evaluating Population Genomic Candidate Genes Underlying Flowering Time in Arabidopsis thaliana Using T-DNA Insertion Lines

2019 ◽  
Vol 110 (4) ◽  
pp. 445-454 ◽  
Author(s):  
Veronica K Chong ◽  
John R Stinchcombe
Keyword(s):  
Arabidopsis Thaliana ◽  
Flowering Time ◽  
Candidate Genes ◽  
Insertional Mutagenesis ◽  
Knock Out ◽  
Population Genomic ◽  
Outlier Loci ◽  
Genomic Studies ◽  
Dna Insertion ◽  
Genomic Scans

Abstract Population genomic scans have emerged as a powerful tool to detect regions of the genome that are potential targets of selection. Despite the success of genomic scans in identifying novel lists of loci potentially underlying adaptation, few studies proceed to validate the function of these candidate genes. In this study, we used transfer-DNA (T-DNA) insertion lines to evaluate the effects of 27 candidate genes on flowering time in North American accessions of Arabidopsis thaliana. We compared the flowering time of T-DNA insertion lines that knock out the function of a candidate gene obtained from population genomic studies to a wild type under long- and short-day conditions. We also did the same for a collection of randomly chosen genes that had not been identified as candidates. We validated the well-known effect of long-day conditions in accelerating flowering time and found that gene disruption caused by insertional mutagenesis tends to delay flowering. Surprisingly, we found that knockouts in random genes were just as likely to produce significant phenotypic effects as knockouts in candidate genes. T-DNA insertions at a handful of candidate genes that had previously been identified as outlier loci showed significant delays in flowering time under both long and short days, suggesting that they are promising candidates for future investigation.

Insertional mutagenesis in Arabidopsis thaliana

Plant Science ◽  
1991 ◽  
Vol 80 (1-2) ◽  
pp. 27-37 ◽  
Author(s):  
M. Van Lijsebettens ◽  
B. den Boer ◽  
J.-P. Hernalsteens ◽  
M. Van Montagu
Keyword(s):  
Arabidopsis Thaliana ◽  
Insertional Mutagenesis

Expression of lavender AGAMOUS-like and SEPALLATA3-like genes promote early flowering and alter leaf morphology in Arabidopsis thaliana

Planta ◽  
2021 ◽  
Vol 254 (3) ◽  
Author(s):  
Ayelign M. Adal ◽  
Elinor Binson ◽  
Lisa Remedios ◽  
Soheil S. Mahmoud
Keyword(s):  
Arabidopsis Thaliana ◽  
Leaf Morphology ◽  
Early Flowering

scholarly journals Female gametophytic mutants of Arabidopsis thaliana identified in a gene trap insertional mutagenesis screen

2011 ◽  
Vol 55 (1) ◽  
pp. 73-84 ◽  
Author(s):  
Vladimir B. Brukhin ◽  
Miloslawa Jaciubek ◽  
Arturo Bolanos Carpio ◽  
Vera Kuzmina ◽  
Ueli Grossniklaus
Keyword(s):  
Arabidopsis Thaliana ◽  
Insertional Mutagenesis ◽  
Gene Trap ◽  
Mutagenesis Screen ◽  
Insertional Mutagenesis Screen

The role of phytochrome C in gravitropism and phototropism in Arabidopsis thaliana

2008 ◽  
Vol 35 (4) ◽  
pp. 298 ◽  
Author(s):  
Prem Kumar ◽  
Crystal E. Montgomery ◽  
John Z. Kiss
Keyword(s):  
Arabidopsis Thaliana ◽  
Blue Light ◽  
Leaf Morphology ◽  
Red Light ◽  
Phytochrome A ◽  
Inflorescence Stems ◽  
Root Gravitropism ◽  
Positive Effect ◽  
Rosette Leaf

The phytochrome (phy) photoreceptors, which consist of a small gene family PHYA-E in dicot plants, play important roles in regulating many light-induced responses in plants. Although the best characterised phytochromes are phytochrome A (phyA) and phytochrome (phyB), the functions of phyD and phyE have been increasingly studied. Phytochrome C (phy C) has been the most poorly understood member of the photoreceptor family, since isolation of phyC mutants only has been accomplished within the last few years. Recent reports show that phyC functions in hypocotyl elongation, rosette leaf morphology, and timing of flowering. In the present study, we show that phyC plays a role in tropisms in seedlings and inflorescence stems of light-grown Arabidopsis thaliana (L.) Heynh. (Wassilewskija ecotype). Phytochrome C has a positive effect on gravitropism in hypocotyls and stems, but it has a limited role in root gravitropism. In contrast, phyC attenuates the positive phototropic response to blue light in hypocotyls and the red-light-based positive phototropism in roots. Phytochrome D (phy D) also mediates gravitropism in hypocotyls and inflorescence stems and attenuates positive phototropism in response to blue in hypocotyls and stems. Thus, phyC can be added to the list of the other four phytochromes, which play various roles in both gravitropism and phototropism in plant organs. This report also supports the growing body of evidence demonstrating cross talk between phytochromes and blue-light photoreceptors.

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