Formation of Hassall's corpuscles in vitro by the thymic epithelial cell line IT-26 R 21 of the rat

1982 ◽  
Vol 226 (3) ◽  
Author(s):  
T. Itoh ◽  
S. Kasahara ◽  
S. Aizu ◽  
K. Kato ◽  
M. Takeuchi ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Thymic Epithelial Cell ◽  
Epithelial Cell Line ◽  
Thymic Epithelial Cell Line

scholarly journals Fetal liver and bone marrow JORO 75+ lymphocyte progenitors are precursors of CD4+8- TCR/CD3- early thymocytes.

1994 ◽  
Vol 179 (2) ◽  
pp. 721-725 ◽  
Author(s):  
E Golunski ◽  
R Palacios
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
Epithelial Cell ◽  
Cell Line ◽  
Fetal Liver ◽  
Cell Receptor ◽  
Thymic Epithelial Cell ◽  
Epithelial Cell Line ◽  
Thymic Epithelial Cell Line

We show here that cell sorter purified JORO 75+ lymphocyte progenitors from fetal liver or bone marrow of adult mice give rise in vitro to CD4+8- T cell receptor (TCR)/CD3- early thymocytes and CD4+8- TCR/CD3+ thymocyte subsets after coculture with the EH6 subcapsular thymic epithelial cell line, recombinant interleukin 7 (rIL-7) and F (supernatants from the FLS4.1 fetal liver stromal cell line). We find that in cultures that had additionally received rIL-2, CD4-8+ TCR/CD3+ cells were also generated. The results strongly suggest that fetal liver and marrow JORO 75+ lymphocyte progenitors are precursors to the early CD4+8- TCR/CD3- intrathymic population previously identified in the adult mouse. The EH6 subcapsular thymic epithelial cell line should facilitate the study of the molecular events responsible for very early stages of T cell development including T lymphocyte-lineage commitment.

scholarly journals Overproduction and secretion of beta 2-microglobulin by a rat thymic epithelial cell line that expresses MHC class I heavy chain

1991 ◽  
Vol 98 (4) ◽  
pp. 559-565
Author(s):  
C. Dargemont ◽  
D. Dunon ◽  
J. Salamero ◽  
M.A. Deugnier ◽  
J. Davoust ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Mhc Class I ◽  
Heavy Chain ◽  
Class I ◽  
Thymic Epithelial Cell ◽  
Epithelial Cell Line ◽  
Beta 2 ◽  
Beta 2 Microglobulin ◽  
Thymic Epithelial Cell Line

Major histocompatibility complex (MHC) class I antigens are constituted of dimers consisting of a peripheral light chain, beta 2-microglobulin (beta 2m) and a transmembrane heavy chain whose cell surface expression depends on its assembly with beta 2m. In contrast, soluble beta 2m can be secreted in the absence of heavy chain expression. The presence of beta 2m in medium conditioned by a rat thymic epithelial cell line, IT45-R1 (IT45) prompted us to investigate whether beta 2m could be secreted by cells that express MHC class I antigens. IT45 cells produce three to five times more beta 2m in the culture supernatant than another rat thymic epithelial cell line, IT26-R21 (IT26). The IT45 cell line exported beta 2m through a constitutive pathway of secretion, as indicated by the kinetics of production and localization of intracellular beta 2m. Although cells from the IT45 cell line expressed a much higher amount of beta 2m as compared to IT26 and NBT II cells (a rat bladder epithelial cell line), all three of these cell lines expressed the same amount of membrane and intracellular MHC class I heavy chain. These data are thus consistent with a constitutive secretion of beta 2m dependent upon an overexpression of MHC class I light chain as compared to the heavy chain. The amount of beta 2m mRNA and the ratio of beta 2m versus MHC class I heavy chain transcripts were higher in IT45 than in IT26 cells, indicating that overexpression of beta 2m in IT45 cells could be due to an enhanced level of beta 2m mRNA.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Positive selection of T-lymphocytes induced by intrathymic injection of a thymic epithelial cell line

Nature ◽  
1992 ◽  
Vol 359 (6397) ◽  
pp. 729-732 ◽  
Author(s):  
Stanislav Vukmanović ◽  
Andres G. Grandea ◽  
Susan J. Faas ◽  
Barbara B. Knowles ◽  
Michael J. Bevan
Keyword(s):  
T Lymphocytes ◽  
Epithelial Cell ◽  
Positive Selection ◽  
Cell Line ◽  
Thymic Epithelial Cell ◽  
Epithelial Cell Line ◽  
Intrathymic Injection ◽  
Thymic Epithelial Cell Line ◽  
Selection Of

Analysis on the types of chemokines expressed by the murine thymic epithelial cell line MTEC1

2000 ◽  
Vol 45 (12) ◽  
pp. 1098-1101 ◽  
Author(s):  
Laiping Xie ◽  
Xiaoping Qian ◽  
Sunyou Gong ◽  
Weifeng Chen
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Thymic Epithelial Cell ◽  
Epithelial Cell Line ◽  
Thymic Epithelial Cell Line
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