Ultrastructural radioautography of synthesis and migration of proteins and catecholamines in the rat adrenal medulla

Sarita Benchimol; Marc Cantin

doi:10.1007/bf00214683

Ultrastructural radioautography of the incorporation of tritiated leucine by the rat adrenal medulla in vivo

Cell and Tissue Research ◽

10.1007/bf00209033 ◽

1978 ◽

Vol 193 (2) ◽

Cited By ~ 2

Author(s):

Sarita Benchimol ◽

Marc Cantin

Keyword(s):

Adrenal Medulla ◽

Ultrastructural Radioautography

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Demonstration of Retinal Glycogen with Silver Methenamine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100066875 ◽

1971 ◽

Vol 29 ◽

pp. 564-565

Author(s):

A. W. Sedar ◽

G. H. Bresnick

Keyword(s):

Lactic Acid ◽

Chromic Acid ◽

Periodic Acid ◽

Müller Cell ◽

External Limiting Membrane ◽

Electron Microscope Level ◽

Reactive Process ◽

Inner Limiting Membrane ◽

Muller Cell ◽

And Migration

After experimetnal damage to the retina with a variety of procedures Müller cell hypertrophy and migration occurs. According to Kuwabara and others the reactive process in these injuries is evidenced by a marked increase in amount of glycogen in the Müller cells. These cells were considered originally supporting elements with fiber processes extending throughout the retina from inner limiting membrane to external limiting membrane, but are known now to have high lactic acid dehydrogenase activity and the ability to synthesize glycogen. Since the periodic acid-chromic acid-silver methenamine technique was shown to demonstrate glycogen at the electron microscope level, it was selected to react with glycogen in the fine processes of the Müller cell that ramify among the neural elements in various layers of the retina and demarcate these cells cytologically. The Rhesus monkey was chosen as an example of a well vascularized retina and the rabbit as an example of a avascular retina to explore the possibilities of the technique.

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Fine Structure of Carotid Body: Normal and Anoxic Cats

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100060763 ◽

1967 ◽

Vol 25 ◽

pp. 150-151

Author(s):

Fadhil Al-Lami ◽

R.G. Murray

Keyword(s):

Fine Structure ◽

Adrenal Medulla ◽

Carotid Body ◽

Uranyl Acetate ◽

Lead Citrate ◽

Glomus Cells ◽

Sustentacular Cells ◽

Carotid Bodies

Although the fine structure of the carotid body has been described in several recent reports, uncertainties remain, and the morphological effects of anoxia on the carotid body cells of the cat have never been reported. We have, therefore, studied the fine structure of the carotid body both in normal and severely anoxic cats, and to test the specificity of the effects, have compared them with the effects on adrenal medulla, kidney, and liver of the same animals. Carotid bodies of 50 normal and 15 severely anoxic cats (9% oxygen in nitrogen) were studied. Glutaraldehyde followed by OsO4 fixations, Epon 812 embedding, and uranyl acetate and lead citrate staining, were the technics employed.We have called the two types of glomus cells enclosed and enclosing cells. They correspond to those previously designated as chemoreceptor and sustentacular cells respectively (1). The enclosed cells forming the vast majority, are irregular in shape with many processes and occasional peripheral densities (Fig. 1).

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Structure and migration of planar defects in crystals observed in atomic scale

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100108568 ◽

1978 ◽

Vol 36 (1) ◽

pp. 284-285 ◽

Cited By ~ 1

Author(s):

H. Hashimoto ◽

Y. Sugimoto ◽

Y. Takai ◽

H. Endoh

Keyword(s):

Electron Microscope ◽

Rotation Angle ◽

Crystal Surface ◽

Electron Gun ◽

Atomic Scale ◽

Present Observation ◽

Twist Boundary ◽

Optical Magnification ◽

Zinc Crystal ◽

And Migration

As was demonstrated by the present authors that atomic structure of simple crystal can be photographed by the conventional 100 kV electron microscope adjusted at “aberration free focus (AFF)” condition. In order to operate the microscope at AFF condition effectively, highly stabilized electron beams with small energy spread and small beam divergence are necessary. In the present observation, a 120 kV electron microscope with LaB6 electron gun was used. The most of the images were taken with the direct electron optical magnification of 1.3 million times and then magnified photographically.1. Twist boundary of ZnSFig. 1 is the image of wurtzite single crystal with twist boundary grown on the surface of zinc crystal by the reaction of sulphur vapour of 1540 Torr at 500°C. Crystal surface is parallel to (00.1) plane and electron beam is incident along the axis normal to the crystal surface. In the twist boundary there is a dislocation net work between two perfect crystals with a certain rotation angle.

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Immunogold labeling of the calcium binding protein synexin in isolated adrenal chromaffin granules and chromaffin cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162326 ◽

1990 ◽

Vol 48 (3) ◽

pp. 952-953

Author(s):

Gemma A.J. Kuijpers ◽

Harvey B. Pollard

Keyword(s):

Adrenal Medulla ◽

Calcium Binding ◽

Chromaffin Cells ◽

Cell Activation ◽

Structural Information ◽

Dependent Manner ◽

Chromaffin Granules ◽

Immuno Electron Microscopy ◽

Ultrathin Sections ◽

Adrenal Chromaffin

Exocytotic fusion of granules in the adrenal medulla chromaffin cell is triggered by a rise in the concentration of cytosolic Ca2+ upon cell activation. The protein synexin, annexin VII, was originally found in the adrenal medulla and has been shown to cause aggregation and to support fusion of chromaffin granules in a Ca2+-dependent manner. We have previously suggested that synexin may there fore play a role in the exocytotic fusion process. In order to obtain more structural information on synexin, we performed immuno-electron microscopy on frozen ultrathin sections of both isolated chromaffin granules and chromaffin cells.Chromaffin granules were isolated from bovine adrenal medulla, and synexin was isolated from bovine lung. Granules were incubated in the presence or absence of synexin (24 μg per mg granule protein) and Ca2+ (1 mM), which induces maximal granule aggregation, in 0.3M sucrose-40m MMES buffer(pH 6.0). Granules were pelleted, washed twice in buffer without synexin and fixed with 2% glutaraldehyde- 2% para formaldehyde in 0.1 M phosphate buffer (GA/PFA) for 30 min. Chromaffin cells were isolated and cultured for 3-5 days, and washed and incubated in Krebs solution with or without 20 uM nicotine. Cells were fixed 90 sec after on set of stimulation with GA/PFA for 30 min. Fixed granule or cell pellets were washed, infiltrated with 2.3 M sucrose in PBS, mounted and frozen in liquid N2.

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