Structure of retinular cells in a Drosophila melanogaster visual mutant, rdgA, at early stages of degeneration

Emiko Matsumoto; Kazushige Hirosawa; Kiyoshi Takagawa; Yoshiki Hotta

doi:10.1007/bf00214371

Caffeine effects on AdoR mRNA expression in Drosophila melanogaster

Open Life Sciences ◽

10.1515/biol-2016-0034 ◽

2016 ◽

Vol 11 (1) ◽

pp. 244-249

Author(s):

Jacek Francikowski ◽

Bartosz Baran ◽

Anna Płachetka-Bożek ◽

Michał Krzyżowski ◽

Maria Augustyniak

Keyword(s):

Drosophila Melanogaster ◽

Mrna Expression ◽

Adenosine Receptors ◽

Fruit Fly ◽

Adult Survival ◽

Cellular Targets ◽

Early Stages ◽

Wide Range ◽

Adult Survival Rate ◽

Mrna Expression Levels

AbstractIn this study, we aimed to evaluate whether exposure to caffeine in the early stages of development affect AdoR mRNA expression levels in the fruit fly (Drosophila melanogaster) and how this will relate to the developmental success of flies. Adenosine receptors are seen as the most important biochemical targets of caffeine. Simultaneously adenosine signaling orchestrates the development and growth of insects. We demonstrate that AdoR mRNA expression in D. melanogaster is persistent from early stages till imago. Strong alterations in AdoR expression were observed in larvae that had been treated with caffeine. However, after the imaginal molt, the differences in AdoR expression between the insects from all of the test groups evened out despite a wide range of developmental success in the groups. Taken together, these results suggest that caffeine affects the expression of its cellular targets even from the early stages of fruit fly development and thus there is a significantly lower larvae-to-adult survival rate. Moreover, we also proved that the expression of AdoR undergoes a peculiar reset during the maturation of D. melanogaster despite the conditions in which larvae developed.

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BEST: A Novel Computational Approach for Comparing Gene Expression Patterns From Early Stages of Drosophila melanogaster Development

Genetics ◽

10.1093/genetics/162.4.2037 ◽

2002 ◽

Vol 162 (4) ◽

pp. 2037-2047

Author(s):

Sudhir Kumar ◽

Karthik Jayaraman ◽

Sethuraman Panchanathan ◽

Rajalakshmi Gurunathan ◽

Ana Marti-Subirana ◽

...

Keyword(s):

Gene Expression ◽

Developmental Biology ◽

Drosophila Melanogaster ◽

Expression Pattern ◽

Early Stage ◽

Expression Patterns ◽

Gene Expression Pattern ◽

Computational Approach ◽

Gene Expression Patterns ◽

Early Stages

Abstract Embryonic gene expression patterns are an indispensable part of modern developmental biology. Currently, investigators must visually inspect numerous images containing embryonic expression patterns to identify spatially similar patterns for inferring potential genetic interactions. The lack of a computational approach to identify pattern similarities is an impediment to advancement in developmental biology research because of the rapidly increasing amount of available embryonic gene expression data. Therefore, we have developed computational approaches to automate the comparison of gene expression patterns contained in images of early stage Drosophila melanogaster embryos (prior to the beginning of germ-band elongation); similarities and differences in gene expression patterns in these early stages have extensive developmental effects. Here we describe a basic expression search tool (BEST) to retrieve best matching expression patterns for a given query expression pattern and a computational device for gene interaction inference using gene expression pattern images and information on the associated genotypes and probes. Analysis of a prototype collection of Drosophila gene expression pattern images is presented to demonstrate the utility of these methods in identifying biologically meaningful matches and inferring gene interactions by direct image content analysis. In particular, the use of BEST searches for gene expression patterns is akin to that of BLAST searches for finding similar sequences. These computational developmental biology methodologies are likely to make the great wealth of embryonic gene expression pattern data easily accessible and to accelerate the discovery of developmental networks.

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Control of Drosophila deoxyuridine triphosphatase. Existence of a developmentally expressed protein inhibitor

Biochemical Journal ◽

10.1042/bj2590593 ◽

1989 ◽

Vol 259 (2) ◽

pp. 593-596 ◽

Cited By ~ 16

Author(s):

M D Nation ◽

S N Guzder ◽

L E Giroir ◽

W A Deutsch

Keyword(s):

Drosophila Melanogaster ◽

Molecular Mass ◽

Sedimentation Coefficient ◽

The Other ◽

Proteinase K ◽

Protein Inhibitor ◽

Stages Of Development ◽

Early Stages ◽

A Subunit ◽

Rnase Treatment

An activity that inhibits deoxyuridine triphosphatase (dUTPase) has been partially purified from Drosophila melanogaster. The inhibitor has a sedimentation coefficient of 4.1 S and a subunit molecular mass of 61 kDa. Its expression is limited to early stages of development, similar to the pattern previously found for dUTPase. The inhibitor is unusually stable to heating and is insensitive to DNAse and RNAse treatment. On the other hand, inhibition is sensitive to digestion with proteinase K, indicating that a protein is required for activity. These results suggest that at least one form of regulation is exerted on Drosophila dUTPase that could allow a greater opportunity for the incorporation of uracil into DNA.

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Female sterile mutations on the second chromosome of Drosophila melanogaster. II. Mutations blocking oogenesis or altering egg morphology.

Genetics ◽

10.1093/genetics/129.4.1119 ◽

1991 ◽

Vol 129 (4) ◽

pp. 1119-1136 ◽

Cited By ~ 23

Author(s):

T Schüpbach ◽

E Wieschaus

Keyword(s):

Drosophila Melanogaster ◽

Embryonic Development ◽

Maternal Effect ◽

Early Stages ◽

Morphological Defects ◽

Egg Morphology ◽

Normal Embryonic Development

Abstract In mutagenesis screens for recessive female sterile mutations on the second chromosome of Drosophila melanogaster 528 lines were isolated which allow the homozygous females to survive but cause sterility. In 62 of these lines early stages of oogenesis are affected, and these females usually do not lay any eggs. In 333 lines oogenesis proceeds apparently normally to stage 8 of oogenesis, but morphological defects become often apparent during later stages of oogenesis, and are visible in the defective eggs produced by these females whereas 133 lay eggs that appear morphologically normal, but do not support normal embryonic development. Of the lines 341 have been genetically characterized and define a total of 140 loci on the second chromosome. Not all the loci are specific for oogenesis. From the numbers obtained we estimate that the second chromosome of Drosophila contains about 13 loci that are relatively specific for early oogenesis, 70 loci that are specifically required in mid to late oogenesis, and around 30 maternal-effect lethals.

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Decarboxylases for polyamine biosynthesis in Drosophila melanogaster larvae

Biochemical Journal ◽

10.1042/bj1540031 ◽

1976 ◽

Vol 154 (1) ◽

pp. 31-33 ◽

Cited By ~ 9

Author(s):

C V Byus ◽

E J Herbst

Keyword(s):

Drosophila Melanogaster ◽

Larval Development ◽

Ornithine Decarboxylase ◽

Enzyme Activities ◽

Polyamine Biosynthesis ◽

Adenosylmethionine Decarboxylase ◽

Larval Stages ◽

Early Stages ◽

Adenosyl Methionine ◽

Stimulation Of

Ornithine decarboxylase (L-ornithine carboxy-lase, EC 4.1.1.17) and S-adenosyl-methionine decarboxylase (S-adenosyl-L-methionine carboxy-lase, EC 4.1.1.50) were assayed in Drosophilia melanogaster larvae. The highest enzyme activities were detected in 24 and 48 h larvae, with diminishing activities in subsequent larval stages. Stimulation of S-adenosylmethionine decarboxylase by putrescine was demonstrable in late but not in early stages of larval development.

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