Characterization of Thinopyrum bessarabicum chromosome segments in wheat using random amplified polymorphic DNAs (RAPDs) and genomic in situ hybridization

1993 ◽  
Vol 86 (8) ◽  
pp. 895-900 ◽  
Author(s):  
I. P. King ◽  
K. A. Purdie ◽  
H. N. Rezanoor ◽  
R. M. D. Koebner ◽  
T. E. Miller ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Genomic In Situ Hybridization ◽  
Thinopyrum Bessarabicum ◽  
Chromosome Segments

Characterization of wheat-Thinopyrum partial amphiploids by meiotic analysis and genomic in situ hybridization

Genome ◽  
2000 ◽  
Vol 43 (4) ◽  
pp. 712-719 ◽  
Author(s):  
George Fedak ◽  
Qin Chen ◽  
Robert L. Conner ◽  
André Laroche ◽  
René Petroski ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Genomic In Situ Hybridization ◽  
Meiotic Analysis ◽  
Partial Amphiploids

Cytological characterization of potato - Solanum etuberosum somatic hybrids and their backcross progenies by genomic in situ hybridization

Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 987-992 ◽  
Author(s):  
F Dong ◽  
R G Novy ◽  
J P Helgeson ◽  
J Jiang
Keyword(s):  
In Situ Hybridization ◽  
Dna Marker ◽  
Somatic Hybrids ◽  
Molecular Cytogenetics ◽  
Genomic In Situ Hybridization ◽  
Preferential Pairing ◽  
Potato Germplasm ◽  
Solanum Etuberosum

Four somatic hybrids derived from a diploid wild species Solanum etuberosum and a diploid tuber-bearing Solanum clone 463-4, together with five BC1 and three BC2 plants, were analyzed by genomic in situ hybridization (GISH). None of the four somatic hybrids had the expected chromosome constitutions, i.e., 24 chromosomes from each fusion parent. Either one chromosome from S. etuberosum or one from the potato parent 463-4 was lost in the hybrids. Three BC1 plants had exactly one set of S. etuberosum chromosomes. The other two BC1 plants either had one extra or one fewer S. etuberosum chromosome, possibly because their somatic hybrid parents had an extra or had lost one S. etuberosum chromosome. The presence of one set, or close to one set, of S. etuberosum chromosomes in all BC1 plants suggests a preferential pairing and segregation of the S. etuberosum chromosomes in the somatic hybrids. Two of the three BC2 plants had 52 chromosomes, deviating significantly from the expected chromosome number of 48. These results suggest poor pairing between S. etuberosum and S. tuberosum chromosomes in the BC1 plants. The present study demonstrates the importance of combining GISH and DNA marker analysis for a thorough characterization of potato germplasm containing chromosomes from different species.Key words: potato germplasm, Solanum etuberosum, molecular cytogenetics.

Genomic in situ hybridization to identify alien chromosomes and chromosome segments in wheat

1992 ◽  
Vol 84-84 (7-8) ◽  
pp. 778-786 ◽  
Author(s):  
T. Schwarzacher ◽  
K. Anamthawat-Jónsson ◽  
G. E. Harrison ◽  
A. K. M. R. Islam ◽  
J. Z. Jia ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Genomic In Situ Hybridization ◽  
Chromosome Segments

Genome analysis of Thinopyrum intermedium and Thinopyrum ponticum using genomic in situ hybridization

Genome ◽  
1998 ◽  
Vol 41 (4) ◽  
pp. 580-586 ◽  
Author(s):  
Qin Chen ◽  
R L Conner ◽  
A Laroche ◽  
J B Thomas
Keyword(s):  
In Situ Hybridization ◽  
Genomic Analysis ◽  
Dna Probes ◽  
Genomic In Situ Hybridization ◽  
Thinopyrum Intermedium ◽  
Thinopyrum Ponticum ◽  
Close Relationship ◽  
Cytogenetic Analyses ◽  
Thinopyrum Bessarabicum

Genomic in situ hybridization (GISH) using genomic DNA probes from Thinopyrum elongatum (Host) D.R. Dewey (genome E, 2n = 14), Thinopyrum bessarabicum (Savul. & Rayss) Á. Löve (genome J, 2n = 14), and Pseudoroegneria strigosa (M. Bieb.) Á. Löve (genome S, 2n = 14), was used to examine the genomic constitution of Thinopyrum intermedium (Host) Barkworth & D.R. Dewey (2n = 6x = 42) and Thinopyrum ponticum (Podp.) Barkworth & D.R. Dewey (2n = 10x = 70). Evidence from GISH indicated that hexaploid Th. intermedium contained the J, Js, and S genomes, in which the J genome was related to the E genome of Th. elongatum and the J genome of Th. bessarabicum. The S genome was homologous to the S genome of Ps. strigosa, while the Js genome referred to modified J- or E-type chromosomes distinguished by the presence of S genome specific sequences close to the centromere. Decaploid Th. ponticum had only the two basic genomes J and Js. The Js genome present in Th. intermedium and Th. ponticum was homologous with E or J genomes, but was quite distinct at centromeric regions, which can strongly hybridize with the S genome DNA probe. Based on GISH results, the genomic formula of Th. intermedium was redesignated JJsS and that of Th. ponticum was redesignated JJJJsJs. The finding of a close relationship among S, J, and Js genomes provides valuable markers for molecular cytogenetic analyses using S genome DNA probes to monitor the transfer of useful traits from Th. intermedium and Th. ponticum to wheat.Key words: genomic in situ hybridization, GISH, Thinopyrum intermedium, Thinopyrum ponticum, genomic analysis, Js genome.

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