Conjugative gene transfer in marine cyanobacteria: Synechococcus sp., Synechocystis sp. and Pseudanabaena sp.

1992 ◽  
Vol 37 (3) ◽  
pp. 369-373 ◽  
Author(s):  
Koji Sode ◽  
Masahiro Tatara ◽  
Haruko Takeyama ◽  
J.Grant Burgess ◽  
Tadashi Matsunaga
Keyword(s):  
Gene Transfer ◽  
Marine Cyanobacteria ◽  
Synechococcus Sp ◽  
Conjugative Gene Transfer ◽  
Synechocystis Sp

The effect of various wavelengths of light from light-emitting diodes on the antioxidant system of marine cyanobacteria, Synechococcus sp.

2013 ◽  
Vol 9 (3) ◽  
pp. 295-302 ◽  
Author(s):  
Cheol Young Choi ◽  
Na Na Kim ◽  
Hyun Suk Shin ◽  
Heum Gi Park ◽  
Sang-Gyu Cheon ◽  
...  
Keyword(s):  
Antioxidant System ◽  
Light Emitting Diodes ◽  
Marine Cyanobacteria ◽  
Light Emitting ◽  
Synechococcus Sp

scholarly journals Genetic Identification of a High-Affinity Ni Transporter and the Transcriptional Response to Ni Deprivation in Synechococcus sp. Strain WH8102

2012 ◽  
Vol 78 (22) ◽  
pp. 7822-7832 ◽  
Author(s):  
C. L. Dupont ◽  
D. A. Johnson ◽  
K. Phillippy ◽  
I. T. Paulsen ◽  
B. Brahamsha ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Gene Knockout ◽  
Transcriptional Response ◽  
Genetic Identification ◽  
Marine Cyanobacteria ◽  
Wild Type ◽  
Intracellular Protein ◽  
Content Type ◽  
Impaired Ability ◽  
Synechococcus Sp

ABSTRACTOne biological need for Ni in marine cyanobacteria stems from the utilization of the Ni metalloenzyme urease for the assimilation of urea as a nitrogen source. In many of the same cyanobacteria, includingSynechococcussp. strain WH8102, an additional and obligate nutrient requirement for Ni results from usage of a Ni superoxide dismutase (Ni-SOD), which is encoded bysodN. To better understand the effects of Ni deprivation on WH8102, parallel microarray-based analysis of gene expression and gene knockout experiments were conducted. The global transcriptional response to Ni deprivation depends upon the nitrogen source provided for growth; fewer than 1% of differentially expressed genes for Ni deprivation on ammonium or urea were concordantly expressed. Surprisingly, genes for putative Ni transporters, including one colocalized on the genome withsodN,sodT, were not induced despite an increase in Ni transport. Knockouts of the putative Ni transporter genesodTappeared to be lethal in WH8102, so the genes forsodTandsodNin WH8102 were interrupted with the gene for Fe-SOD,sodB, and its promoter fromSynechococcussp. strain WH7803. ThesodT::sodBexconjugants were unable to grow at low Ni concentrations, confirming that SodT is a Ni transporter. ThesodN::sodBexconjugants displayed higher growth rates at low Ni concentrations than did the wild type, presumably due to a relaxed competition between urease and Ni-SOD for Ni. BothsodT::sodBandsodN::sodBlines exhibited an impaired ability to grow at low Fe concentrations. We propose a posttranslational allosteric SodT regulation involving the binding of Ni to a histidine-rich intracellular protein loop.

Recipe for standard BG-11 media v1

2021 ◽  
Author(s):  
Anna Behle ◽  
Alice Pawlowski
Keyword(s):  
Citric Acid ◽  
Final Concentration ◽  
Ammonium Citrate ◽  
Marine Cyanobacteria ◽  
Pure Cultures ◽  
Synechocystis Sp ◽  
Pcc 6803

Stanier RY, Deruelles J, Rippka R, Herdman M, Waterbury JB: Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 1979, 111:1–61. Recipes for standard and alternative BG11 for culturing freshwater cyanobacteria, such as Synechocystis sp. PCC 6803, as described. Media is usually not suitable for marine cyanobacteria. Final Concentration of Medium. CaCl2*2 H2O 0.036 g/L Citric acid 0.006 g/L NaNO3 1.4958 g/L MgSO4* 7 H2O 0.0749 g/L 0.25M Na2EDTA (pH 8) 0.0056 mL/L Na2CO3 20 µg/ml Fe(III) Ammonium citrate 6 µg/ml K2HPO4 * 3H2O 30 µg/ml TES Buffer (pH 8) 10 mM H3BO3 2.86 mg/L MnCl2 * 4 H2O 1.81 mg/L ZnSO4 * 7 H2O 0.222 mg/L Na2MoO4 * 2 H2O 0.390 mg/L Co(NO3)2 *6 H2O 0.049 mg/L (CuSO4 * 5 H2O 0.079 mg/L if required)

scholarly journals Comparative study of the different mechanisms for zinc ion stress sensing in two cyanobacterial strains, Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803

BIOPHYSICS ◽  
2012 ◽  
Vol 8 (0) ◽  
pp. 103-109 ◽  
Author(s):  
Eugene Hayato Morita ◽  
Satsuki Kawamoto ◽  
Shunnosuke Abe ◽  
Yoshitaka Nishiyama ◽  
Takahisa Ikegami ◽  
...  
Keyword(s):  
Comparative Study ◽  
Zinc Ion ◽  
Synechococcus Sp ◽  
Stress Sensing ◽  
Pcc 7942 ◽  
Synechocystis Sp ◽  
Pcc 6803

Kinetics of conjugative gene transfer on surfaces in granular porous media

2010 ◽  
Vol 112 (1-4) ◽  
pp. 91-102 ◽  
Author(s):  
A. Massoudieh ◽  
C. Crain ◽  
E. Lambertini ◽  
K.E. Nelson ◽  
T. Barkouki ◽  
...  
Keyword(s):  
Porous Media ◽  
Gene Transfer ◽  
Granular Porous Media ◽  
Conjugative Gene Transfer ◽  
Kinetics Of

scholarly journals Chemical reactivity of Synechococcus sp. PCC 7002 and Synechocystis sp. PCC 6803 hemoglobins: covalent heme attachment and bishistidine coordination

2011 ◽  
Vol 16 (4) ◽  
pp. 539-552 ◽  
Author(s):  
Henry J. Nothnagel ◽  
Matthew R. Preimesberger ◽  
Matthew P. Pond ◽  
Benjamin Y. Winer ◽  
Emily M. Adney ◽  
...  
Keyword(s):  
Chemical Reactivity ◽  
Synechococcus Sp ◽  
Covalent Heme Attachment ◽  
Synechocystis Sp ◽  
Pcc 6803
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