Cytochemical localization of adenylate cyclase activity in the rat anterior pituitary

1981 ◽  
Vol 218 (1) ◽  
Author(s):  
Masato Komuro ◽  
Toshiro Shioda
Keyword(s):  
Adenylate Cyclase ◽  
Anterior Pituitary ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Cytochemical Localization

Adenosine and its analogue ( − )-N6-R-phenyl-isopropyladenosine modulate anterior pituitary adenylate cyclase activity and prolactin secretion in the rat

1990 ◽  
Vol 5 (1) ◽  
pp. 69-76 ◽  
Author(s):  
G. Schettini ◽  
E. Landolfi ◽  
O. Meucci ◽  
T. Florio ◽  
M. Grimaldi ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Anterior Pituitary ◽  
Primary Cultures ◽  
Prolactin Secretion ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Pituitary Cells ◽  
Prolactin Release ◽  
Low Concentrations ◽  
High Concentrations

ABSTRACT The effect of adenosine and its analogue ( − )-N6-R-phenylisopropyladenosine (PIA) on both anterior pituitary adenylate cyclase activity and prolactin secretion was examined in the rat. Adenosine inhibited basal adenylate cyclase activity in a dose-dependent manner and also reduced the stimulation of the enzyme by vasoactive intestinal peptide (VIP). Likewise, in primary cultures of anterior pituitary cells, adenosine decreased prolactin secretion in both basal and VIP-stimulated conditions. In perifusion experiments, adenosine also inhibited prolactin release in both basal and TRH-stimulated conditions. PIA produced a biphasic pattern of response of basal adenylate cyclase activity, being inhibitory at low and stimulatory at high concentrations. In VIP-stimulated conditions, low concentrations of PIA inhibited both adenylate cyclase activity and prolactin release from primary cultures of pituitary cells, while no additive stimulatory effect was seen at high concentrations. Similarly, low concentrations of PIA reduced both basal and TRH-stimulated prolactin release from perifused pituitaries, while increasing PIA concentrations restored prolactin release. These data show that adenosine affects basal and stimulated prolactin secretion from anterior pituitary cells. Adenosine receptors seem to be coupled to the adenylate cyclase system in the anterior pituitary gland, suggesting a possible relationship between the effect of adenosine on adenylate cyclase activity and prolactin secretion.

Somatostatin inhibition of anterior pituitary adenylate cyclase activity: different sensitivity between male and female rats

1988 ◽  
Vol 439 (1-2) ◽  
pp. 322-329 ◽  
Author(s):  
Gennaro Schettini ◽  
Tullio Florio ◽  
Olimpia Meucci ◽  
Elisa Landolfi ◽  
Gaetano Lombardi ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Anterior Pituitary ◽  
Female Rats ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Male And Female ◽  
Male And Female Rats ◽  
Pituitary Adenylate Cyclase
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