The adoptive immunotherapy of cancer using lymphokine activated killer cells and recombinant interleukin-2

1986 ◽  
Vol 9 (1) ◽  
Author(s):  
StephenE. Ettinghausen ◽  
StevenA. Rosenberg
Keyword(s):  
Adoptive Immunotherapy ◽  
Interleukin 2 ◽  
Lymphokine Activated Killer Cells ◽  
Killer Cells ◽  
Recombinant Interleukin 2 ◽  
Immunotherapy Of Cancer

Interstitial Nephritis in a Patient Receiving Adoptive Immunotherapy with Recombinant Interleukin-2 and Lymphokine-Activated Killer Cells

1991 ◽  
Vol 11 (6) ◽  
pp. 489-492 ◽  
Author(s):  
Donald A. Feinfeld ◽  
Vivette D’Agati ◽  
Janice P. Dutcher ◽  
Steven B. Werfel ◽  
Robert I. Lynn ◽  
...  
Keyword(s):  
Interstitial Nephritis ◽  
Adoptive Immunotherapy ◽  
Interleukin 2 ◽  
Lymphokine Activated Killer Cells ◽  
Killer Cells ◽  
Recombinant Interleukin 2

A new Procedure for Large Scale Production and Freezing of Lymphokine Activated Killer (LAK) Cells to be used in Adoptive Immunotherapy of Cancer

1988 ◽  
Vol 74 (5) ◽  
pp. 523-530 ◽  
Author(s):  
Carlo Gambacorti-Passerini ◽  
Marina Radrizzani ◽  
Licia Rivoltini ◽  
Edoardo Marchesi ◽  
Fernando Ravagnani ◽  
...  
Keyword(s):  
Adoptive Immunotherapy ◽  
Large Scale ◽  
Interleukin 2 ◽  
Peripheral Blood Lymphocytes ◽  
Cell Factory ◽  
Scale Production ◽  
Lymphokine Activated Killer Cells ◽  
Large Scale Production ◽  
Recombinant Interleukin 2 ◽  
Immunotherapy Of Cancer

A new procedure for activation of peripheral blood lymphocytes (PBL) with recombinant interleukin 2 (rIL2) is described. PBL obtained by leukapheresis were subjected to NH4Cl (ACK) treatment to clear erythrocyte contamination; Ficoll separation was not performed. PBL were subsequently seeded in 10-floor multitrays (Cell Factory™, CF), gasified and incubated at 37 °C for 3-4 days in a humidified 5% CO2 atmosphere. This procedure achieved an activation (evaluated as cytotoxicity and proliferation) comparable with that obtained by culturing PBL in small flasks. Optimal activation of PBL was achieved in CF even in the presence of granulocyte contamination of up to 40%. It was also possible to freeze, thaw and recover most of the frozen cells and their cytotoxic activity. With this procedure therefore large quantities of lymphokine activated killer cells (LAK) can be easily produced to be used in adoptive immunotherapy trials.

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