Temperature-conditional nuclear mutation of Chlamydomonas reinhardtii decreases the CO2/O2 specificity of chloroplast ribulosebisphosphate carboxylase/oxygenase

Planta ◽  
1994 ◽  
Vol 193 (3) ◽  
Author(s):  
Cecilia Gotor ◽  
Seokjoo Hong ◽  
RobertJ. Spreitzer
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Nuclear Mutation ◽  
Ribulosebisphosphate Carboxylase

scholarly journals Stress induces the assembly of RNA granules in the chloroplast of Chlamydomonas reinhardtii

2008 ◽  
Vol 182 (4) ◽  
pp. 641-646 ◽  
Author(s):  
James Uniacke ◽  
William Zerges
Keyword(s):  
Oxidative Stress ◽  
Chlamydomonas Reinhardtii ◽  
Large Subunit ◽  
Ribosomal Subunit ◽  
Stress Granules ◽  
Mrna Localization ◽  
Messenger Rnas ◽  
Rna Granules ◽  
Cytoplasmic Rna ◽  
Ribulosebisphosphate Carboxylase

Eukaryotic cells under stress repress translation and localize these messenger RNAs (mRNAs) to cytoplasmic RNA granules. We show that specific stress stimuli induce the assembly of RNA granules in an organelle with bacterial ancestry, the chloroplast of Chlamydomonas reinhardtii. These chloroplast stress granules (cpSGs) form during oxidative stress and disassemble during recovery from stress. Like mammalian stress granules, cpSGs contain poly(A)-binding protein and the small, but not the large, ribosomal subunit. In addition, mRNAs are in continuous flux between polysomes and cpSGs during stress. Localization of cpSGs within the pyrenoid reveals that this chloroplast compartment functions in this stress response. The large subunit of ribulosebisphosphate carboxylase/oxygenase also assembles into cpSGs and is known to bind mRNAs during oxidative stress, raising the possibility that it plays a role in cpSG assembly. This discovery within such an organelle suggests that mRNA localization to granules during stress is a more general phenomenon than currently realized.

scholarly journals Mating Type Linked Mutations Which Disrupt the Uniparental Transmission of Chloroplast Genes in Chlamydomonas

Genetics ◽  
1987 ◽  
Vol 115 (4) ◽  
pp. 677-684
Author(s):  
Nicholas W Gillham ◽  
John E Boynton ◽  
Anita M Johnson ◽  
Bryce D Burkhart
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Mating Type ◽  
Mitochondrial Genomes ◽  
Chloroplast Gene ◽  
Chloroplast Genes ◽  
Nuclear Mutation ◽  
High Transmission ◽  
Chloroplast Genomes ◽  
Nuclear Mutations ◽  
As If

ABSTRACT In Chlamydomonas reinhardtii, chloroplast genomes are normally transmitted by the mating type plus (mt  +) parent and mitochondrial genomes by the mating type minus (mt  -) parent. In this paper we describe three new nuclear mutations, designated mat-3-1 to -3, which are tightly linked to the mt  + allele and permit high transmission of chloroplast genomes from the mt  - parent, but have no effect on transmission of mitochondrial genomes. We also show that mat-1, reported by others to be a nuclear mutation linked to mt  - which promotes transmission of chloroplast genomes by the mt  - parent, is probably a vegetative diploid since it contains both mt  + and mt  - alleles. Vegetative diploids behave as if they are mt  - with respect to mating, but posses a level of chloroplast gene transmission intermediate between that of haploid mt  - and mt  + stocks.

A nuclear mutation affects the synthesis of the chloroplast psbA gene production Chlamydomonas reinhardtii

1992 ◽  
Vol 22 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Jacqueline Girard-Bascou ◽  
Yves Pierre ◽  
Dominique Drapier
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Psba Gene ◽  
Nuclear Mutation
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