Quantification of indole-3-acetic acid in untransformed and Agrobacterium rhizogenes-transformed pea roots using gas chromatography mass spectrometry

Planta ◽  
1993 ◽  
Vol 189 (1) ◽  
Author(s):  
Santiago Schaerer ◽  
Paul-Emile Pilet
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Acetic Acid ◽  
Agrobacterium Rhizogenes ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Pea Roots ◽  
Indole 3 Acetic Acid

scholarly journals Identification of New Metabolites of Bacterial Transformation of Indole by Gas Chromatography-Mass Spectrometry and High Performance Liquid Chromatography

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Pankaj Kumar Arora ◽  
Hanhong Bae
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
High Performance ◽  
Glyoxylic Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Indole 3 Acetic Acid

Arthrobactersp. SPG transformed indole completely in the presence of an additional carbon source. High performance liquid chromatography and gas chromatography-mass spectrometry detected indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde as biotransformation products. This is the first report of the formation of indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde from indole by any bacterium.

scholarly journals GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF AN ENDANGERED MEDICINAL PLANT, SARCOSTEMMA VIMINALE (L.) R.BR. FROM THAR DESERT, RAJASTHAN (INDIA)

2017 ◽  
Vol 10 (9) ◽  
pp. 210 ◽  
Author(s):  
Arora Sunita ◽  
Meena Sonam ◽  
Kumar Ganesh
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Acetic Acid ◽  
Medicinal Plants ◽  
Maximum Amount ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
World Population ◽  
Thar Desert ◽  
Chromatography Mass Spectrometry

  Objective: Sarcostemma viminale (L.) R. Br. is one of the important endangered medicinal plants belonging to the family Asclepiadaceae. The aim of the present investigation was to determine the possible bioactive phytochemicals from stem of S. viminale (L.) R. Br. using methanol, chloroform, and hexane as solvents.Methods: Plant material was collected from typical conditions of Indian Thar Desert in the month of July-September, 2016. This plant always grows in association with the congeneric plant, Euphorbia caducifolia. The phytochemical compounds were investigated using Perkin-Elmer gas chromatography-mass spectrometry, while the mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology library.Results: Maximum % area is found for Lup-20-(29)-en-3-yl acetate is present maximum amount (40.85%) with reaction time (RT)=43.787 minutes, followed by 4, 4, 6A, 6B, 8A, 11, 11, 14B-octamethyl-1, 4, 4A, 5, 6, 6A, 6B, 7, 8, 8A, 9, 10, 11, 12, 12A, 14, 14A, 14B-octadecahydro-2H-picen-3- one$$olean-12-en-3-one# (13.74%) with RT=44.420 minutes in the methanolic extract; acetic acid 4, 4, 6A, 8A, 11, 12, 14B-octamethyl-1, 2, 3, 4, 4A, 5, 6, 6A, 6B, 7, 8, 8A, 9, 10, 11, 12, 12A, 14, 14A, 14B-eicosahydro-picen-3-yl ester $$ urs-12-en-3-yl acetate is present maximum amount (44.98%) with RT=48.265 minutes, followed by. beta.-amyrin (18.51%) with RT=40.580 minutes in the chloroform extract; acetic acid 4, 4, 6A, 8A, 11, 12, 14B-octamethyl-1, 2, 3, 4, 4A, 5, 6, 6A, 6B, 7, 8, 8A, 9, 10, 11, 12, 12A, 14, 14A, 14B-eicosahydro-picen-3-yl ester $$ urs-12-en-3-yl acetate is present maximum amount (45.47%) with RT=48.514 minutes, followed by. beta.-amyrin (19.21%) with RT=40.555 minutes in the hexane extract of stem of S. viminale (L.) R. Br.Conclusion: Medicinal plants contain one or more substances that can be used for therapeutic purpose; they are used by the world population for their basic health needs. The importance of the study is to investigate the pinpoint biological activity of some of these compounds so that they can be used by pharma or some other drug designing industry to find a novel drug.

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