The CO2 permeability of the plasma membrane of Chlamydomonas reinhardtii: mass-spectrometric 18O-exchange measurements from 13C18O2 in suspensions of carbonic anhydrase-loaded plasma-membrane vesicles

Planta ◽  
1996 ◽  
Vol 200 (3) ◽  
Author(s):  
Dieter S�ltemeyer ◽  
Karl-Albert Rinast
Keyword(s):  
Plasma Membrane ◽  
Carbonic Anhydrase ◽  
Chlamydomonas Reinhardtii ◽  
Membrane Vesicles ◽  
Mass Spectrometric ◽  
Plasma Membrane Vesicles

Involvement of H+-ATPase and carbonic anhydrase in inorganic carbon uptake for endosymbiont photosynthesis

2000 ◽  
Vol 278 (4) ◽  
pp. R870-R881 ◽  
Author(s):  
Paola Furla ◽  
Denis Allemand ◽  
Maria-Novella Orsenigo
Keyword(s):  
Plasma Membrane ◽  
Carbonic Anhydrase ◽  
Inorganic Carbon ◽  
Carbonic Acid ◽  
Membrane Vesicles ◽  
Allyl Isothiocyanate ◽  
Membrane Diffusion ◽  
Plasma Membrane Vesicles ◽  
Animal Cells ◽  
Inorganic Carbon Transport

Symbiotic cnidarians absorb inorganic carbon from seawater to supply intracellular dinoflagellates with CO2 for their photosynthesis. To determine the mechanism of inorganic carbon transport by animal cells, we used plasma membrane vesicles prepared from ectodermal cells isolated from tentacles of the sea anemone, Anemonia viridis. H14 [Formula: see text] uptake in the presence of an outward NaCl gradient or inward H+ gradient, showed no evidence for a Cl−- or H+- driven [Formula: see text] transport. H14 [Formula: see text] and36Cl− uptakes were stimulated by a positive inside-membrane diffusion potential, suggesting the presence of [Formula: see text] and Cl−conductances. A carbonic anhydrase (CA) activity was measured on plasma membrane (4%) and in the cytoplasm of the ectodermal cells (96%) and was sensitive to acetazolamide (IC50 = 20 nM) and ethoxyzolamide (IC50 = 2.5 nM). A strong DIDS-sensitive H+-ATPase activity was observed (IC50 = 14 μM). This activity was also highly sensitive to vanadate and allyl isothiocyanate, two inhibitors of P-type H+-ATPases. Present data suggest that [Formula: see text]absorption by ectodermal cells is carried out by H+secretion by H+-ATPase, resulting in the formation of carbonic acid in the surrounding seawater, which is quickly dehydrated into CO2 by a membrane-bound CA. CO2 then diffuses passively into the cell where it is hydrated in[Formula: see text] by a cytosolic CA.

Calcium efflux of plasma membrane vesicles exposed to ELF magnetic fields-test of a nuclear magnetic resonance interaction model

2012 ◽  
Vol 33 (7) ◽  
pp. 535-542 ◽  
Author(s):  
Wenjun J. Sun ◽  
Mehri Kaviani Mogadam ◽  
Marianne Sommarin ◽  
Henrietta Nittby ◽  
Leif G. Salford ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Plasma Membrane ◽  
Magnetic Resonance ◽  
Magnetic Fields ◽  
Interaction Model ◽  
Membrane Vesicles ◽  
Resonance Interaction ◽  
Calcium Efflux ◽  
Plasma Membrane Vesicles ◽  
Nuclear Magnetic

Pore-Spanning Plasma Membranes Derived from Giant Plasma Membrane Vesicles

2021 ◽  
Author(s):  
Nikolas K. Teiwes ◽  
Ingo Mey ◽  
Phila C. Baumann ◽  
Lena Strieker ◽  
Ulla Unkelbach ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Plasma Membranes ◽  
Membrane Vesicles ◽  
Plasma Membrane Vesicles
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