In vivo generation of lymphokine activated killer cell activity by ABPP and interleukin-2 and their antitumor effects against immunogenic and nonimmunogenic tumors in murine tumor models

1988 ◽  
Vol 26 (1) ◽  
Author(s):  
AlexanderM.M. Eggermont ◽  
PaulH. Sugarbaker ◽  
RichardL. Marquet ◽  
Johannes Jeekel
Keyword(s):  
Killer Cell ◽  
Interleukin 2 ◽  
Cell Activity ◽  
Tumor Models ◽  
Antitumor Effects ◽  
Lymphokine Activated Killer Cell ◽  
Murine Tumor ◽  
Killer Cell Activity

Generation of lymphokine-activated killer cell activity by low-dose recombinant interleukin-2 and tumor cells

1990 ◽  
Vol 128 (2) ◽  
pp. 516-527 ◽  
Author(s):  
Saburo Yamamoto ◽  
Dave S.B. Hoon ◽  
Peter Chandler ◽  
Ingrid Schmid ◽  
Reiko F. Irie
Keyword(s):  
Tumor Cells ◽  
Low Dose ◽  
Killer Cell ◽  
Interleukin 2 ◽  
Cell Activity ◽  
Lymphokine Activated Killer Cell ◽  
Killer Cell Activity ◽  
Recombinant Interleukin 2

scholarly journals Poxvirus-Induced Immunostimulating Effects on Porcine Leukocytes

2000 ◽  
Vol 74 (17) ◽  
pp. 7943-7951 ◽  
Author(s):  
Vicky Fachinger ◽  
Tobias Schlapp ◽  
Walter Strube ◽  
Norbert Schmeer ◽  
Armin Saalmüller
Keyword(s):  
Natural Killer Cell Activity ◽  
Killer Cell ◽  
Interleukin 2 ◽  
Cell Activity ◽  
Basic Mechanism ◽  
Killer Cell Activity ◽  
Contrast Enhanced ◽  
Measured Flow

ABSTRACT The prophylactic application of inactivated parapox ovis viruses (Baypamun; Bayer AG, Leverkusen, Germany) has been shown to reduce efficiently the outbreak of stress-mediated diseases in different species. However, little is known about the basic mechanism behind this observed stimulatory property. We therefore tested eight inactivated poxvirus strains belonging to three different genera (Orthopoxvirus, Avipoxvirus, andParapoxvirus) for their capacity to activate cells of the porcine innate and specific immune systems in vitro. The results indicated that poxviruses failed to induce increased phagocytosis, oxidative burst, or natural killer cell activity in swine. In contrast, enhanced release of interleukin-2, alpha interferon, and gamma interferon, as well as strong proliferation, could be measured. Flow cytometric analyses and cell sorting experiments identified T-helper cells as the main target responding to inactivated poxviruses: the activated cells had a CD4high CD25+ major histocompatibility complex type II-positive phenotype and were the major source of secreted cytokines. Together, the results demonstrated that all tested poxviruses possessed immunostimulating capacity. These in vitro poxvirus-induced effects may be responsible at least in part for the in vivo immunostimulating capacity of inactivated poxviruses.

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