Oxyntic cell differentiation during physiological cell renewal in abomasal mucosa of adult cattle

1996 ◽  
Vol 193 (3) ◽  
Author(s):  
Monika Kressin
Keyword(s):  
Cell Differentiation ◽  
Cell Renewal ◽  
Adult Cattle ◽  
Oxyntic Cell

scholarly journals Transcription profiling of cultured Acropora digitifera adult cells reveals the existence of ancestral genome regulatory modules underlying pluripotency and cell differentiation in cnidaria

2021 ◽  
Author(s):  
Alejandro Reyes-Bermudez ◽  
Michio Hidaka ◽  
andAlexander Mikheyev
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Gene Networks ◽  
Expression Profiles ◽  
Regulatory Gene ◽  
Stem Cell Markers ◽  
Cell Renewal ◽  
Ancestral Genome ◽  
Regulatory Modules ◽  
Acropora Digitifera

Abstract Due to their pluripotent nature and unlimited cell renewal, stem cells have been proposed as an ideal material for establishing long-term cnidarian cell cultures. However, the lack of unifying principles associated with "stemness" across the phylum complicates stem cells' identification and isolation. Here, we for the first time report gene expression profiles for cultured coral cells, focusing on regulatory gene networks underlying pluripotency and differentiation. Cultures were initiated from Acropora digitifera tip fragments, the fastest growing tissue in Acropora. Overall, in vitro transcription resembled early larvae, over-expressing orthologs of pre-metazoan and Hydra stem cell markers, and transcripts with roles in cell division, migration, and differentiation. Our results suggest the presence of pluripotent cell-types in cultures and indicate the existence of ancestral genome regulatory modules underlying pluripotency and cell differentiation in cnidaria. Cultured cells appear to be synthesizing protein, differentiating, and proliferating.

scholarly journals Generation of Differentiating and Long-Living Intestinal Organoids Reflecting the Cellular Diversity of Canine Intestine

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 822 ◽  
Author(s):  
Nina Kramer ◽  
Barbara Pratscher ◽  
Andre M. C. Meneses ◽  
Waltraud Tschulenk ◽  
Ingrid Walter ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Culture Conditions ◽  
Cell Renewal ◽  
Lineage Differentiation ◽  
Promising Tool ◽  
Intestinal Organoids ◽  
Induced Apoptosis ◽  
And Function

Functional intestinal disorders constitute major, potentially lethal health problems in humans. Consequently, research focuses on elucidating the underlying pathobiological mechanisms and establishing therapeutic strategies. In this context, intestinal organoids have emerged as a potent in vitro model as they faithfully recapitulate the structure and function of the intestinal segment they represent. Interestingly, human-like intestinal diseases also affect dogs, making canine intestinal organoids a promising tool for canine and comparative research. Therefore, we generated organoids from canine duodenum, jejunum and colon, and focused on simultaneous long-term expansion and cell differentiation to maximize applicability. Following their establishment, canine intestinal organoids were grown under various culture conditions and then analyzed with respect to cell viability/apoptosis and multi-lineage differentiation by transcription profiling, proliferation assay, cell staining, and transmission electron microscopy. Standard expansion medium supported long-term expansion of organoids irrespective of their origin, but inhibited cell differentiation. Conversely, transfer of organoids to differentiation medium promoted goblet cell and enteroendocrine cell development, but simultaneously induced apoptosis. Unimpeded stem cell renewal and concurrent differentiation was achieved by culturing organoids in the presence of tyrosine kinase ligands. Our findings unambiguously highlight the characteristic cellular diversity of canine duodenum, jejunum and colon as fundamental prerequisite for accurate in vitro modelling.

Cell renewal, cell differentiation and programmed cell death (apoptosis) in pilomatrixoma

1997 ◽  
Vol 137 (5) ◽  
pp. 714-720 ◽  
Author(s):  
A. FAYYAZI ◽  
A. SORURI ◽  
H.J. RADZUN ◽  
J.H. PETERS ◽  
H. BERGER
Keyword(s):  
Cell Death ◽  
Cell Differentiation ◽  
Programmed Cell Death ◽  
Cell Renewal

Cell renewal, cell differentiation and programmed cell death (apoptosis) in pilomatrixoma

1997 ◽  
Vol 137 (5) ◽  
pp. 714-720 ◽  
Author(s):  
A. FAYYAZI ◽  
A. SORURI ◽  
H.J. RADZUN ◽  
J.H. PETERS ◽  
H. BERGER
Keyword(s):  
Cell Death ◽  
Cell Differentiation ◽  
Programmed Cell Death ◽  
Cell Renewal

Electron microscope study of the secretory activity of epithelial cells in embryonic thymus

1990 ◽  
Vol 48 (3) ◽  
pp. 382-383
Author(s):  
H. Alasam
Keyword(s):  
Cell Differentiation ◽  
T Cell ◽  
Epithelial Cells ◽  
Electron Density ◽  
Secretory Activity ◽  
T Cell Differentiation ◽  
High Electron ◽  
Transmission Electron ◽  
Medullary Epithelial Cells ◽  
Thymic Factor

The possibility that intrathymic T-cell differentiation involves stem cell-lymphoid interactions in embryos led us to study the ultrastructure of epithelial cell in normal embryonic thymus. Studies in adult thymus showed that it produces several peptides that induce T-cell differentiation. Several of them have been chemically characterized, such as thymosin α 1, thymopoietin, thymic humoral factor or the serum thymic factor. It was suggested that most of these factors are secreted by populations of A and B-epithelial cells.Embryonic materials were obtained from inbred matings of Swiss Albino mice. Thymuses were disected from embryos 17 days old and prepared for transmission electron microscopy. Our studies showed that embryonic thymus at this stage contains undifferentiated and differentiated epithelial cells, large lymphoblasts, medium and few small lymphocytes (Fig. 5). No differences were found between cortical and medullary epithelial cells, in contrast to the findings of Van Vliet et al,. Epithelial cells were mostly of the A-type with low electron density in both cytoplasm and nucleus. However few B-type with high electron density were also found (Fig. 7).

Role of CD5 expression on TCRγδ T cell-differentiation and development of chronic intestinal inflammation

2001 ◽  
Vol 120 (5) ◽  
pp. A517-A517
Author(s):  
A MIZOGUCHI ◽  
E MIZOGUCHI ◽  
Y DEJONG ◽  
H TAKEDATSU ◽  
F PREFFER ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
T Cell ◽  
Intestinal Inflammation ◽  
T Cell Differentiation ◽  
Chronic Intestinal Inflammation

Cell renewal in human epidermis

1965 ◽  
Vol 92 (4) ◽  
pp. 462-468 ◽  
Author(s):  
W. L. Epstein
Keyword(s):  
Human Epidermis ◽  
Cell Renewal
Sign in / Sign up

Export Citation Format

Share Document