Preparation of sealed tonoplast and plasma-membrane vesicles from Catharanthus roseus (L.) G. Don. cells by free-flow electrophoresis

Planta ◽  
1991 ◽  
Vol 184 (4) ◽  
Author(s):  
Herv� Canut ◽  
Sylvie Baudracco ◽  
Mireille Caban� ◽  
Alain-Michel Boudet ◽  
G�rard Marigo
Keyword(s):  
Plasma Membrane ◽  
Catharanthus Roseus ◽  
Membrane Vesicles ◽  
Free Flow ◽  
Plasma Membrane Vesicles

scholarly journals Plasma Membrane Vesicles of Opposite Sidedness from Soybean Hypocotyls by Preparative Free-Flow Electrophoresis

1988 ◽  
Vol 86 (2) ◽  
pp. 631-637 ◽  
Author(s):  
Hervé Canut ◽  
Andrew Brightman ◽  
Alain M. Boudet ◽  
D. James Morré
Keyword(s):  
Plasma Membrane ◽  
Membrane Vesicles ◽  
Free Flow ◽  
Plasma Membrane Vesicles

scholarly journals Free-Flow Electrophoresis of Plasma Membrane Vesicles Enriched by Two-Phase Partitioning Enhances the Quality of the Proteome from Arabidopsis Seedlings

2016 ◽  
Vol 15 (3) ◽  
pp. 900-913 ◽  
Author(s):  
Roberto de Michele ◽  
Heather E. McFarlane ◽  
Harriet T. Parsons ◽  
Miranda J. Meents ◽  
Jeemeng Lao ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Vesicles ◽  
Free Flow ◽  
Plasma Membrane Vesicles ◽  
Two Phase ◽  
Phase Partitioning

scholarly journals Calcium ion transport across plasma membranes isolated from rat kidney cortex

1979 ◽  
Vol 178 (3) ◽  
pp. 549-557 ◽  
Author(s):  
P Gmaj ◽  
H Murer ◽  
R Kinne
Keyword(s):  
Plasma Membrane ◽  
Plasma Membranes ◽  
Rat Kidney ◽  
Membrane Vesicles ◽  
Free Flow ◽  
Kidney Cortex ◽  
Plasma Membrane Vesicles ◽  
Filtration Method ◽  
Rat Kidney Cortex ◽  
Calcium Ion Transport

Basal-lateral-plasma-membrane vesicles and brush-border-membrane vesicles were isolated from rat kidney cortex by differential centrifugation followed by free-flow-electrophoresis. Ca2+ uptake into these vesicles was investigated by a rapid filtration method. Both membranes show a considerable binding of Ca2+ to the vesicle interior, making the analysis of passive fluxes in uptake experiments difficult. Only the basal-lateral-plasma-membrane vesicles exhibit an ATP-dependent pump activity which can be distinguished from the activity in mitochondrial and endoplasmic reticulum by virtue of the different distribution during free-flow electrophoresis and its lack of sensitivity to oligomycin. The basal-lateral plasma membranes contain in addition a Na+/Ca2+-exchange system which mediates a probably rheogenic counter-transport of Ca2+ and Na+ across the basal cell border. The latter system is probably involved in the secondary active Na+-dependent and ouabain-inhibitable Ca2+ reabsorption in the proximal tubule, the ATP-driven system is probably more important for the maintenance of a low concentration of intracellular Ca2+.

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