scholarly journals 1H, 15N, 13C and 13CO assignments and secondary structure determination of basic fibroblast growth factor using 3D heteronuclear NMR spectroscopy

1995 ◽  
Vol 6 (3) ◽  
Author(s):  
FranklinJ. Moy ◽  
AndrewP. Seddon ◽  
ErnestB. Campbell ◽  
Peter B�hlen ◽  
Robert Powers
Keyword(s):  
Nmr Spectroscopy ◽  
Growth Factor ◽  
Secondary Structure ◽  
Fibroblast Growth Factor ◽  
Structure Determination ◽  
Heteronuclear Nmr ◽  
Fibroblast Growth ◽  
Heteronuclear Nmr Spectroscopy ◽  
3D Heteronuclear Nmr

scholarly journals Secondary structure determination of 15 N-labelled human Long-[Arg-3]-insulin-like growth factor 1 by multidimensional NMR spectroscopy

FEBS Letters ◽  
1997 ◽  
Vol 420 (1) ◽  
pp. 97-102 ◽  
Author(s):  
Leanne G Laajoki ◽  
Eugene Le Breton ◽  
Gary K Shooter ◽  
John C Wallace ◽  
Geoffrey L Francis ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Growth Factor ◽  
Secondary Structure ◽  
Structure Determination ◽  
Multidimensional Nmr ◽  
Insulin Like Growth Factor ◽  
Multidimensional Nmr Spectroscopy

Correlation of wing-leg identity in ectopic FGF-induced chimeric limbs with the differential expression of chick Tbx5 and Tbx4

Development ◽  
1998 ◽  
Vol 125 (1) ◽  
pp. 51-60 ◽  
Author(s):  
H. Ohuchi ◽  
J. Takeuchi ◽  
H. Yoshioka ◽  
Y. Ishimaru ◽  
K. Ogura ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Expression Patterns ◽  
Ectopic Expression ◽  
Limb Bud ◽  
Chick Embryos ◽  
Fibroblast Growth ◽  
Limb Buds ◽  
Specific Position

It has been reported that members of the fibroblast growth factor (FGF) family can induce additional limb formation in the flank of chick embryos. The phenotype of the ectopic limb depends on the somite level at which it forms: limbs in the anterior flank resemble wings, whereas those in the posterior flank resemble legs. Ectopic limbs located in the mid-flank appear chimeric, possessing characteristics of both wings and legs; feather buds are present in the anterior halves with scales and claws in the posterior halves. To study the mechanisms underlying the chimerism of these additional limbs, we cloned chick Tbx5 and Tbx4 to use as forelimb and hindlimb markers and examined their expression patterns in FGF-induced limb buds. We found that Tbx5 and Tbx4 were differentially expressed in the anterior and posterior halves of additional limb buds in the mid-flank, respectively, consistent with the chimeric patterns of the integument. A boundary of Tbx5/Tbx4 exists in all ectopic limbs, indicating that the additional limbs are essentially chimeric, although the degree of chimerism is dependent on the position. The boundary of Tbx5/Tbx4 expression is not fixed at a specific position within the interlimb region, but dependent upon where FGF was applied. Since the ectopic expression patterns of Tbx5/Tbx4 in the additional limbs are closely correlated with the patterns of their chimeric phenotypes, it is likely that Tbx5 and Tbx4 expression in the limb bud is involved in determination of the forelimb and hindlimb identities, respectively, in vertebrates.

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