Fruit removal in soybean induces the formation of an insoluble form of ribulose-1,5-bisphosphate carboxylase/oxygenase in leaf extracts*

Planta ◽  
1991 ◽  
Vol 183 (2) ◽  
Author(s):  
StevenJ. Crafts-Brandner ◽  
MichaelE. Salvucci ◽  
DennisB. Egli
Keyword(s):  
Leaf Extracts ◽  
Fruit Removal ◽  
Bisphosphate Carboxylase ◽  
Insoluble Form

scholarly journals Measurement and Preservation of the in Vivo Activation of Ribulose 1,5-Bisphosphate Carboxylase in Leaf Extracts

1982 ◽  
Vol 69 (5) ◽  
pp. 1165-1168 ◽  
Author(s):  
John T. Perchorowicz ◽  
Deborah A. Raynes ◽  
Richard G. Jensen
Keyword(s):  
Leaf Extracts ◽  
Bisphosphate Carboxylase

Regulation of Rubisco activity during grain-fill in maize: possible role of Rubisco activase

1997 ◽  
Vol 128 (2) ◽  
pp. 155-161 ◽  
Author(s):  
E. MARTÍNEZ-BARAJAS ◽  
J. MOLINA-GALÁN ◽  
E. SÁNCHEZ de JIMÉNEZ
Keyword(s):  
Soluble Protein ◽  
Grain Filling ◽  
Rubisco Activase ◽  
Middle Part ◽  
Ribulose Bisphosphate Carboxylase ◽  
Rubisco Activity ◽  
Leaf Extracts ◽  
Bisphosphate Carboxylase ◽  
Grain Filling Period

Levels of ribulose bisphosphate carboxylase (Rubisco) and Rubisco activase were compared in leaves above the ear in two genetically related populations (Z0 and Z20) of maize (Zea mays L.). Z20 was obtained from Z0 after twenty agronomic selection cycles for grain yield improvement (c. 90% above Z0). Plants were cultivated in the highlands of Mexico and leaves were sampled weekly during the grain-filling period. Chlorophyll, soluble protein and Rubisco activity were measured. Chlorophyll and soluble protein content slowly decreased during this period, the former faster than the latter, with no significant differences between populations. During the first 40 days after anthesis, Rubisco activity was significantly greater in the high-yielding population (Z20), although Western blot analysis of Rubisco showed similar values for both populations within this period. However, the same analysis for Rubisco activase indicated a greater amount of this protein in the higher-yielding population (Z20) than the original one (Z0) during the early and middle part of the grain-filling period. The addition of Rubisco activase and an ATP-generating system to Z0 leaf extracts resulted in increased Rubisco activity. It was concluded that during grain-fill in maize, the level of Rubisco activase has a regulatory effect on Rubisco activity expression.

Three-dimensional distribution of ribulose-1, 5-bisphosphate carboxylase/oxygemase during the early development of proplastids in dark-grown Euglena cells transferred to an inorganic medium

1990 ◽  
Vol 48 (3) ◽  
pp. 906-907
Author(s):  
Tomoko Ehara ◽  
Shuji Sumida ◽  
Tetsuaki Osafune ◽  
Eiji Hase
Keyword(s):  
Cell Suspension ◽  
Euglena Gracilis ◽  
Carbon Materials ◽  
Three Dimensional ◽  
Peripheral Region ◽  
Plastid Development ◽  
Bisphosphate Carboxylase ◽  
Inorganic Medium ◽  
Ribulose 1 ◽  
Dimensional Distribution

As shown previously, Euglena cells grown in Hutner’s medium in the dark without agitation accumulate wax as well as paramylum, and contain proplastids showing no internal structure except for a single prothylakoid existing close to the envelope. When the cells are transferred to an inorganic medium containing ammonium salt and the cell suspension is aerated in the dark, the wax was oxidatively metabolized, providing carbon materials and energy 23 for some dark processes of plastid development. Under these conditions, pyrenoid-like structures (called “pro-pyrenoids”) are formed at the sites adjacent to the prolamel larbodies (PLB) localized in the peripheral region of the proplastid. The single prothylakoid becomes paired with a newly formed prothylakoid, and a part of the paired prothylakoids is extended, with foldings, in to the “propyrenoid”. In this study, we observed a concentration of RuBisCO in the “propyrenoid” of Euglena gracilis strain Z using immunoelectron microscopy.

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