Analysis of late-blight disease resistance and freezing tolerance in transgenic potato plants expressing sense and antisense genes for an osmotin-like protein

Planta ◽  
1996 ◽  
Vol 198 (1) ◽  
Author(s):  
Baolong Zhu ◽  
TonyH.H. Chen ◽  
PaulH. Li
1995 ◽  
Vol 7 (9) ◽  
pp. 1357 ◽  
Author(s):  
Gusui Wu ◽  
Barry J. Shortt ◽  
Ellen B. Lawrence ◽  
Elaine B. Levine ◽  
Karen C. Fitzsimmons ◽  
...  

Biology ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 952
Author(s):  
Arinaitwe Abel Byarugaba ◽  
Gerald Baguma ◽  
Douglas Mutebi Jjemba ◽  
Aharinta Kenneth Faith ◽  
Arthur Wasukira ◽  
...  

Transgenic potato event Vic.172, expressing three naturally occurring resistance genes (R genes) conferring complete protection against late blight disease, was evaluated for resistance to late blight, phenotypic characterization, and agronomic performance in field conditions at three locations during three seasons in Uganda. These trials were conducted by comparison to the variety Victoria from which Vic.172 derives, using identical fungicide treatment, except when evaluating disease resistance. During all seasons, the transgenic event Vic.172 was confirmed to have complete resistance to late blight disease, whereas Victoria plants were completely dead by 60–80 days after planting. Tubers from Vic.172 were completely resistant to LB after artificial inoculation. The phenotypic characterization included observations of the characteristics and development of the stems, leaves, flowers, and tubers. Differences in phenotypic parameters between Vic.172 and Victoria were not statistically significant across locations and seasons. The agronomic performance observations covered sprouting, emergence, vigor, foliage growth, and yield. Differences in agronomic performance were not statistically significant except for marketable yield in one location under high productivity conditions. However, yield variation across locations and seasons was not statistically significant, but was influenced by the environment. Hence, the results of the comparative assessment of the phenotype and agronomic performance revealed that transgenic event Vic.172 did not present biologically significant differences in comparison to the variety Victoria it derives from.


2016 ◽  
Vol 16 (2) ◽  
pp. 51
Author(s):  
Edy Listanto ◽  
Eny Ida Riyanti ◽  
Tri Joko Santoso ◽  
Toto Hadiarto ◽  
A. Dinar Ambarwati

Development of potato cultivars with high levels of broad spectrum resistance is a key long-term management strategy against late blight disease caused by Phytophthora infestans. Six progeny lines of hybridization between transgenic potato Katahdin SP951 with non-transgenic Granola and Atlantic were selected based on agronomical characteristics and resistance to late blight disease. The study aimed to analyze the number of insertions and stability of inserted RB gene in the transgenic potato lines. The research was carried out through plant DNA extraction, southern blot analysis and polymerase chain reaction (PCR). Southern blot analysis was used to detect the number of inserts integrated into potato genome, while PCR analysis was used to detect stability of RB gene from generation to generation. The results showed that the progenies obtained from hybridization between Atlantic and transgenic Katahdin SP951 (lines No. 20 and 27) and between Granola and transgenic Katahdin SP951 (line No. 69) contained one copy number of RB gene, according to the probing of nptII. The result is similar to that of inserted RB gene found in the parental transgenic Katahdin SP951. The presence of RB gene in four different generations (G0, G1, G2 and G3) showed stable integration of the gene into the plant genome. The single copy number of RB gene will repress the occurrence of silencing gene expression. The stability analysis of RB gene can determine that the gene is still present in plant genome after several generations.


2016 ◽  
Vol 16 (2) ◽  
pp. 51 ◽  
Author(s):  
Edy Listanto ◽  
Eny Ida Riyanti ◽  
Tri Joko Santoso ◽  
Toto Hadiarto ◽  
A. Dinar Ambarwati

Development of potato cultivars with high levels of broad spectrum resistance is a key long-term management strategy against late blight disease caused by Phytophthora infestans. Six progeny lines of hybridization between transgenic potato Katahdin SP951 with non-transgenic Granola and Atlantic were selected based on agronomical characteristics and resistance to late blight disease. The study aimed to analyze the number of insertions and stability of inserted RB gene in the transgenic potato lines. The research was carried out through plant DNA extraction, southern blot analysis and polymerase chain reaction (PCR). Southern blot analysis was used to detect the number of inserts integrated into potato genome, while PCR analysis was used to detect stability of RB gene from generation to generation. The results showed that the progenies obtained from hybridization between Atlantic and transgenic Katahdin SP951 (lines No. 20 and 27) and between Granola and transgenic Katahdin SP951 (line No. 69) contained one copy number of RB gene, according to the probing of nptII. The result is similar to that of inserted RB gene found in the parental transgenic Katahdin SP951. The presence of RB gene in four different generations (G0, G1, G2 and G3) showed stable integration of the gene into the plant genome. The single copy number of RB gene will repress the occurrence of silencing gene expression. The stability analysis of RB gene can determine that the gene is still present in plant genome after several generations.


1995 ◽  
Vol 7 (9) ◽  
pp. 1357-1368 ◽  
Author(s):  
G Wu ◽  
B J Shortt ◽  
E B Lawrence ◽  
E B Levine ◽  
K C Fitzsimmons ◽  
...  

2020 ◽  
Vol 47 (2) ◽  
pp. 157-163
Author(s):  
Hyeong Cheol Park ◽  
Hyun Jin Chun ◽  
Min Chul Kim ◽  
Sin Woo Lee ◽  
Woo Sik Chung

2021 ◽  
Vol 74 (1) ◽  
pp. 181-187
Author(s):  
Mehi Lal ◽  
Sorabh Chaudhary ◽  
Sanjay Rawal ◽  
Sanjeev Sharma ◽  
Manoj Kumar ◽  
...  

Planta ◽  
1999 ◽  
Vol 210 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Sophia Biemelt ◽  
Mohammad Reza Hajirezaei ◽  
Michael Melzer ◽  
Gerd Albrecht ◽  
Uwe Sonnewald

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