Membrane binding sites for the human blood group H-type 2 determinant, an inducer of laminarinase activity in protoplasts of Rubus fruticosus L.

Planta ◽  
1992 ◽  
Vol 188 (4) ◽  
Author(s):  
Y. Li�nart ◽  
R. Dubois-Dauphin ◽  
C. Gautier ◽  
M. Khitri ◽  
H. Driguez
Keyword(s):  
Blood Group ◽  
Human Blood ◽  
Binding Sites ◽  
Membrane Binding ◽  
Human Blood Group ◽  
Rubus Fruticosus ◽  
Blood Group H ◽  
Membrane Binding Sites

Heterogeneity of carbohydrate fragments isolated from human blood group H and Lea active glycoproteins by base-borohydride degradation

Biochemistry ◽  
1973 ◽  
Vol 12 (10) ◽  
pp. 1955-1961 ◽  
Author(s):  
Luciana Rovis ◽  
Byron Anderson ◽  
Elvin A. Kabat ◽  
Flavio Gruezo ◽  
Jerry Liao
Keyword(s):  
Blood Group ◽  
Human Blood ◽  
Human Blood Group ◽  
Blood Group H

Molecular recognition XI. The synthesis of extensively deoxygenated derivatives of the H-type 2 human blood group determinant and their binding by an anti-H-type 2 monoclonal antibody and the lectin 1 of Ulexeuropaeus

1992 ◽  
Vol 70 (1) ◽  
pp. 254-271 ◽  
Author(s):  
Ulrike Spohr ◽  
Eugenia Paszkiewicz-Hnatiw ◽  
Naohiko Morishima ◽  
Raymond U. Lemieux
Keyword(s):  
Monoclonal Antibody ◽  
Molecular Recognition ◽  
Blood Group ◽  
Human Blood ◽  
Water Molecules ◽  
Hydroxyl Groups ◽  
Human Blood Group ◽  
Derivatives Of ◽  
Hydrogen Bonded

The relative potencies of a wide variety of deoxygenated derivatives of the methyl glycoside of α-L-Fuc-(1 → 2)-β-D-Gal-(1 → 4)- β-D-GlcNAc (the H-type 2 human blood group related trisaccharide) for the inhibition of the binding of an artificial H-type 2 antigen by the lectin I of Ulexeuropaeus confirmed the previous evidence that the key and productive interaction involves only the three hydroxyl groups of the α-L-fucose unit, the hydroxyl at the 3-position of the β-D-galactose residue, and the nonpolar groups in their immediate environment. Except for the acetamido group and the hydroxymethyl of the β-D-Gal unit, which stay in the aqueous phase, on complex formation the remaining three hydroxyl groups appear to come to reside at or near the periphery of the combining site since their replacement by hydrogen causes relatively small changes (< ± 1 kcal/mol) in the stability of the complex (ΔG0). Relatively much larger but compensating changes occur for the enthalpy and entropy terms, and these may arise primarily from the differences in the water structure about the periphery of the combining site and the oligosaccharide both prior to and after complexation. It is proposed that steric constraints lead to an ordered state of the water molecules hydrogen-bonded to the polar groups within the cleft formed by the key region of the amphiphilic combining site. Their release to form less ordered clusters of more strongly hydrogen-bonded water molecules in bulk solution would contribute importantly to the driving force for complexation. It is demonstrated that the surface used for the binding of H-type 2-OMe by a monoclonal anti-H antibody is virtually identical to that used by the Ulex lectin. Keywords: molecular recognition, H-type 2 blood group determinant and deoxygenated derivatives, lectin I of Ulexeuropaeus, anti-H-type 2 monoclonal antibody, enthalpy–entropy compensation.

A highly convergent synthesis of an N-linked glycopeptide presenting the H-type 2 human blood group determinant

Tetrahedron ◽  
2006 ◽  
Vol 62 (20) ◽  
pp. 4954-4978 ◽  
Author(s):  
Zhi-Guang Wang ◽  
J. David Warren ◽  
Vadim Y. Dudkin ◽  
Xufang Zhang ◽  
Ulrich Iserloh ◽  
...  
Keyword(s):  
Blood Group ◽  
Human Blood ◽  
Convergent Synthesis ◽  
Human Blood Group

ChemInform Abstract: Toward Fully Synthetic Homogeneous Glycoproteins: A High Mannose Core Containing Glycopeptide Carrying Full H-Type 2 Human Blood Group Specificity.

ChemInform ◽  
2010 ◽  
Vol 32 (34) ◽  
pp. no-no
Author(s):  
Zhi-Guang Wang ◽  
Xufang Zhang ◽  
Michael Visser ◽  
David Live ◽  
Andrzej Zatorski ◽  
...  
Keyword(s):  
Blood Group ◽  
Human Blood ◽  
Human Blood Group ◽  
Group Specificity ◽  
High Mannose

scholarly journals The one-pot multienzyme (OPME) synthesis of human blood group H antigens and a human milk oligosaccharide (HMOS) with highly active Thermosynechococcus elongatus α1–2-fucosyltransferase

2016 ◽  
Vol 52 (20) ◽  
pp. 3899-3902 ◽  
Author(s):  
Chao Zhao ◽  
Yijing Wu ◽  
Hai Yu ◽  
Ishita M. Shah ◽  
Yanhong Li ◽  
...  
Keyword(s):  
Human Milk ◽  
Blood Group ◽  
Human Blood ◽  
One Pot ◽  
Human Blood Group ◽  
Milk Oligosaccharide ◽  
Highly Active ◽  
Human Milk Oligosaccharide ◽  
The One ◽  
Blood Group H

LNFP I and human blood H group antigens were synthesized efficientlyviaone-pot multienzyme (OPME) fucosylation with a bacterial α1–2-fucosyltransferase.

Molecular recognition XIII. The binding of the H-type 2 human blood group determinant by a winged bean (Psophocarpus tetragonolobus) acidic lectin

1994 ◽  
Vol 72 (1) ◽  
pp. 158-163 ◽  
Author(s):  
Raymond U. Lemieux ◽  
Ming-Hui Du ◽  
Ulrike Spohr ◽  
Shreeta Acharya ◽  
Avadhesha Surolia
Keyword(s):  
Blood Group ◽  
Human Blood ◽  
Acetyl Derivative ◽  
Bulk Water ◽  
Winged Bean ◽  
Hydroxyl Groups ◽  
Human Blood Group ◽  
Methyl Derivatives

The winged bean acidic lectin (WBA II) binds the H-type 2 human blood group related trisaccharide (α-L-Fuc-(1c → 2b)-β-D-Gal-(1b → 4a)-β-D-GlcNAc-OMe, 1). Interactions that importantly contribute to the specificity of the complex formation are provided by CH2-6b, OH-4b, OH-3b, and OH-2c of 1. On the basis of the relative activities of the monodeoxy and mono-O-methyl derivatives of 1, the hydroxyl groups at the 3a, 6a, and 4c positions become located at the periphery of the combining site, whereas the CH3O-1a, NHAc-2a, and CH3-5c groups are more remote from the protein surface and fully exposed to bulk water. Whereas the WBA II lectin marginally recognizes the H-type 1 related trisaccharide (α-L-Fuc-(1c → 2b)-β-D-Gal-(1b → 3a)-β-D-GlcNAc-OMe, 23), the de-N-acetyl derivative (24) is bound 3.4 times more strongly than 1. This and other results, which are related to changes in interaction between the ligand and the apron of the combining site, are attributed to changes in hydration that lead to enthalpy–entropy compensation. In certain cases, the interactions are found to stabilize the complex.

Conversion of the human blood group H antigen to A antigen in vitro

1991 ◽  
Vol 69 (2) ◽  
pp. 111-118 ◽  
Author(s):  
NAN YANG ◽  
BARRY BOETTCHER
Keyword(s):  
Blood Group ◽  
Human Blood ◽  
Human Blood Group ◽  
Blood Group H
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