Intracellular carbonic anhydrase activities in Dunaliella tertiolecta (Butcher) and Chlamydomonas reinhardtii (Dangeard) in relation to inorganic carbon concentration during growth: further evidence for the existence of two distinct carbonic anhydrases associated with the chloroplasts

Planta ◽  
1996 ◽  
Vol 199 (2) ◽  
Author(s):  
Gabi Amoroso ◽  
Claudia Weber ◽  
Dieter S�ltemeyer ◽  
Heinrich Fock
Keyword(s):  
Carbonic Anhydrase ◽  
Chlamydomonas Reinhardtii ◽  
Carbon Concentration ◽  
Inorganic Carbon ◽  
Dunaliella Tertiolecta ◽  
Carbonic Anhydrases ◽  
Inorganic Carbon Concentration

Carbonic anhydrase in eukaryotic algae: characterization, regulation, and possible function during photosynthesis

1998 ◽  
Vol 76 (6) ◽  
pp. 962-972 ◽  
Author(s):  
Dieter Sültemeyer
Keyword(s):  
Carbonic Anhydrase ◽  
Carbon Concentration ◽  
Inorganic Carbon ◽  
Molecular Level ◽  
Carbonic Anhydrases ◽  
Ph Values ◽  
Genes Encoding ◽  
Inorganic Carbon Concentration ◽  
Macro Algae ◽  
Made In

Carbonic anhydrase (CA) speeds up the equilibrium between CO2 and HCO3- at physiological pH values and has been detected in almost every species of the animal and plant kingdoms. Among eucaryotic micro- and macro-algae the enzyme is widely distributed and plays an important role in photosynthetic CO2 fixation. In some cases, different forms of carbonic anhydrases located extracellularly and intracellularly have been found to occur in the same cell. The expression of the genes encoding these CA isoforms are under the control of the inorganic carbon concentration in the medium, as the activities increase with decreasing the inorganic carbon content. Considerable progress has been made in recent years in isolating and characterizing the various forms of carbonic anhydrases on a biochemical and molecular level. Most of the data have been collected for microalgae like Chlamydomonas reinhardtii (Dangeard), while the situation in macroalgae is still descriptive. Therefore, this review summarizes the recent development with an emphasis on microalgae carbonic anhydrases.Key words: carbonic anhydrase, CO2 concentrating mechanism, macroalgae, microalgae, photosynthesis.

Isolation of chloroplast envelopes from Dunaliella tertiolecta

1998 ◽  
Vol 76 (6) ◽  
pp. 1146-1152
Author(s):  
Arun Goyal ◽  
Jens Thielmann ◽  
N E Tolbert
Keyword(s):  
Membrane Proteins ◽  
Carbon Concentration ◽  
Inorganic Carbon ◽  
Dissolved Inorganic Carbon ◽  
Dunaliella Tertiolecta ◽  
Integral Membrane Proteins ◽  
High Yield ◽  
Induced Proteins ◽  
Inner Envelope

Techniques have been developed to obtain Dunaliella chloroplasts and their outer and inner envelopes in high yield. With purified envelopes, we have identified two integral membrane proteins that are specifically induced by low CO2. These two proteins are located in the inner envelope, which is the proposed site for the active dissolved inorganic carbon transporter in unicellular green algae.Key words: algae, carbon concentration mechanism, chloroplast envelopes, dissolved inorganic carbon, Dunaliella, low CO2 induced proteins.

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