Expression of F?f-3 in relation to hindbrain segmentation, otic pit position and pharyngeal arch morphology in normal and retinoic acid-exposed mouse embryos

1996 ◽  
Vol 194 (1) ◽  
Author(s):  
Radma Mahmood ◽  
IvorJ. Mason ◽  
GillianM. Morriss-Kay
Keyword(s):  
Retinoic Acid ◽  
Mouse Embryos ◽  
Pharyngeal Arch

The Synthetic Retinoid AGN 193109 but Not Retinoic Acid Elevates CYP1A1 Levels in Mouse Embryos and Hepa-1c1c7 Cells

2001 ◽  
Vol 174 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Dianne Robert Soprano ◽  
Carlo J. Gambone ◽  
Sabina N. Sheikh ◽  
Jerome L. Gabriel ◽  
Roshantha A.S. Chandraratna ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Mouse Embryos ◽  
Synthetic Retinoid

Otx2andGbx2are required for refinement and not induction of mid-hindbrain gene expression

Development ◽  
2001 ◽  
Vol 128 (24) ◽  
pp. 4979-4991 ◽  
Author(s):  
James Y. H. Li ◽  
Alexandra L. Joyner
Keyword(s):  
Gene Expression ◽  
Retinoic Acid ◽  
Mouse Embryos ◽  
Anterior Region ◽  
Spatial Domains ◽  
Cerebellum Development

Otx2 and Gbx2 are among the earliest genes expressed in the neuroectoderm, dividing it into anterior and posterior domains with a common border that marks the mid-hindbrain junction. Otx2 is required for development of the forebrain and midbrain, and Gbx2 for the anterior hindbrain. Furthermore, opposing interactions between Otx2 and Gbx2 play an important role in positioning the mid-hindbrain boundary, where an organizer forms that regulates midbrain and cerebellum development. We show that the expression domains of Otx2 and Gbx2 are initially established independently of each other at the early headfold stage, and then their expression rapidly becomes interdependent by the late headfold stage. As we demonstrate that the repression of Otx2 by retinoic acid is dependent on an induction of Gbx2 in the anterior brain, molecules other than retinoic acid must regulate the initial expression of Otx2 in vivo. In contrast to previous suggestions that an interaction between Otx2- and Gbx2-expressing cells may be essential for induction of mid-hindbrain organizer factors such as Fgf8, we find that Fgf8 and other essential mid-hindbrain genes are induced in a correct temporal manner in mouse embryos deficient for both Otx2 and Gbx2. However, expression of these genes is abnormally co-localized in a broad anterior region of the neuroectoderm. Finally, we find that by removing Otx2 function, development of rhombomere 3 is rescued in Gbx2–/– embryos, showing that Gbx2 plays a permissive, not instructive, role in rhombomere 3 development. Our results provide new insights into induction and maintenance of the mid-hindbrain genetic cascade by showing that a mid-hindbrain competence region is initially established independent of the division of the neuroectoderm into an anterior Otx2-positive domain and posterior Gbx2-positive domain. Furthermore, Otx2 and Gbx2 are required to suppress hindbrain and midbrain development, respectively, and thus allow establishment of the normal spatial domains of Fgf8 and other genes.

Fgf8is required for pharyngeal arch and cardiovascular development in the mouse

Development ◽  
2002 ◽  
Vol 129 (19) ◽  
pp. 4613-4625 ◽  
Author(s):  
Radwan Abu-Issa ◽  
Graham Smyth ◽  
Ida Smoak ◽  
Ken-ichi Yamamura ◽  
Erik N. Meyers
Keyword(s):  
Craniofacial Development ◽  
Mouse Embryos ◽  
Pharyngeal Arch ◽  
Compound Heterozygous ◽  
Cardiovascular Development ◽  
Early Analysis ◽  
Pharyngeal Arches ◽  
The Face ◽  
Cardiovascular Defects ◽  
Additional Role

We present here an analysis of cardiovascular and pharyngeal arch development in mouse embryos hypomorphic for Fgf8. Previously, we have described the generation of Fgf8 compound heterozygous (Fgf8neo/–) embryos. Although early analysis demonstrated that some of these embryos have abnormal left-right (LR) axis specification and cardiac looping reversals, the number and type of cardiac defects present at term suggested an additional role for Fgf8 in cardiovascular development. Most Fgf8neo/– mutant embryos survive to term with abnormal cardiovascular patterning, including outflow tract, arch artery and intracardiac defects. In addition, these mutants have hypoplastic pharyngeal arches, small or absent thymus and abnormal craniofacial development. Neural crest cells (NCCs) populate the pharyngeal arches and contribute to many structures of the face, neck and cardiovascular system, suggesting that Fgf8 may be required for NCC development. Fgf8 is expressed within the developing pharyngeal arch ectoderm and endoderm during NCC migration through the arches. Analysis of NCC development in Fgf8neo/– mutant embryos demonstrates that NCCs are specified and migrate, but undergo cell death in areas both adjacent and distal to where Fgf8 is normally expressed. This study defines the cardiovascular defects present in Fgf8 mutants and supports a role for Fgf8 in development of all the pharyngeal arches and in NCC survival.

Suppression of erythroid cell differentiation in mouse embryos exposed to retinoic acid in utero

1989 ◽  
Vol 223 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Yoshiko Yasuda ◽  
Hiroyoshi Konishi ◽  
Takuya Matsuo ◽  
Takashi Tanimura
Keyword(s):  
Retinoic Acid ◽  
Cell Differentiation ◽  
In Utero ◽  
Mouse Embryos ◽  
Erythroid Cell ◽  
Erythroid Cell Differentiation

scholarly journals Nuclear Detection of Cellular Retinoic Acid Binding Proteins I and II with New Antibodies

1998 ◽  
Vol 46 (10) ◽  
pp. 1103-1111 ◽  
Author(s):  
Marie-Pierre Gaub ◽  
Yves Lutz ◽  
Norbert B. Ghyselinck ◽  
Isabelle Scheuer ◽  
Véronique Pfister ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Binding Proteins ◽  
Polyclonal Antibodies ◽  
Amino Acid Sequences ◽  
Mouse Embryos ◽  
Low Molecular Weight ◽  
E Coli ◽  
Nuclear Receptor Family ◽  
Retinoic Acid Binding Proteins ◽  
Receptor Family

Apart from the retinoic acid nuclear receptor family, there are two low molecular weight (15 kD) cellular retinoic acid binding proteins, named CRABPI and II. Mouse monoclonal and rabbit polyclonal antibodies were raised against these proteins by using as antigens either synthetic peptides corresponding to amino acid sequences unique to CRABPI or CRABPII, or purified CRABP proteins expressed in E. coli. Antibodies specific for mouse and/or human CRABPI and CRABPII were obtained and characterized by immunocytochemistry and immunoblotting. They allowed the detection not only of CRABPI but also of CRABPII in both nuclear and cytosolic extracts from transfected COS-1 cells, mouse embryos, and various cell lines.

scholarly journals GATA3 is essential for separating patterning domains during facial morphogenesis

Development ◽  
2021 ◽  
Author(s):  
Makoto Abe ◽  
Timothy C. Cox ◽  
Anthony B. Firulli ◽  
Stanley M. Kanai ◽  
Jacob Dalhka ◽  
...  
Keyword(s):  
Gene Regulatory Networks ◽  
Regulatory Networks ◽  
Mouse Embryos ◽  
Pharyngeal Arch ◽  
Bony Fusion ◽  
Craniofacial Microsomia ◽  
Crucial Component ◽  
Gene Regulatory ◽  
Facial Development ◽  
Gata Family

Neural crest cells (NCCs) within the mandibular and maxillary prominences of the first pharyngeal arch are initially competent to respond to signals from either region. However, mechanisms that are only partially understood establish developmental tissue boundaries to ensure spatially correct patterning. In the Hinge and Caps model of facial development, signals from both ventral prominences (the caps) pattern the adjacent tissues while the intervening region, referred to as the maxillomandibular junction (the hinge), maintains separation of the mandibular and maxillary domains. One cap signal is GATA3, a member of the GATA family of zinc-finger transcription factors with a distinct expression pattern in the ventral-most part of the mandibular and maxillary portions of the first arch. Here we show that disruption of Gata3 in mouse embryos leads to craniofacial microsomia and syngnathia (bony fusion of the upper and lower jaws) that results from changes in BMP4 and FGF8 gene regulatory networks within NCCs near the maxillomandibular junction. GATA3 is thus a crucial component in establishing the network of factors that functionally separate the upper and lower jaws during development.

scholarly journals THE EXPRESSION OF CELLULAR RETINOIC ACID BINDING PROTEIN (CRABP) IN DEVELOPING HEART AND SKELETAL MUSCLES OF MOUSE EMBRYOS

1991 ◽  
Vol 12 (2) ◽  
pp. 105-112 ◽  
Author(s):  
KOU ICHIHASHI ◽  
MARIKO MOMOI ◽  
TAKANORI YAMAGATA ◽  
MASAYOSHI YANAGISAWA ◽  
TAKASHI MOMOI
Keyword(s):  
Retinoic Acid ◽  
Skeletal Muscles ◽  
Binding Protein ◽  
Mouse Embryos ◽  
Acid Binding Protein ◽  
Developing Heart
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